Production of polysaccharide slime by microbial mats in the hypersaline environment of a Western Australian solar saltfield

Roux, Jean-Maxime

doi:10.1007/bf01995826

Cited by 32 publications

(21 citation statements)

References 25 publications

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“…Nitrogen deficiency stimulates polysaccharide production, as it has been demonstrated in Merismopedia tenuisima (Konopka and Schnur, 1981), Synechococcus (Roux, 1996), Cyanothece sp. , Anabaena Singh and Das, 2011), Nostoc (Otero and Vincenzini, 2003;Otero and Vincenzini, 2004;Singh and Das, 2011) and Oscillatoria (Jindal, 2011).…”

Section: Discussionmentioning

confidence: 80%

COMPARATIVE GROWTH AND BIOCHEMICAL COMPOSITION OF FOUR STRAINS OF Nostoc AND Anabaena (CYANOBACTERIA, NOSTOCALES) IN RELATION TO SODIUM NITRATE

et al. 2016

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Nitrogen concentration is an essential parameter in cyanobacterial cultures to produce enriched biomass with biotechnological purposes. Growth and biochemical composition of Nostoc LAUN0015, Nostoc UAM206, Anabaena sp.1 and Anabaena sp.2 were compared at 0, 4.25, 8.5 and 17 mM NaNO 3 . Cultures under laboratory conditions were maintained for 30 days at a volume of 500 mL. Anabaena sp.1 yielded the highest value of dry mass of 0.26 ± 2.49 mg mL -1 at 8.5 mM NaNO 3 . For chlorophyll, phycocyanin and phycoerythrin, maximum values were achieved at 17 mM NaNO 3 with 18.09 ± 1.74, 102.90 ± 6.73 and 53.47 ± 2.40 μg mL -1 , respectively. Nostoc LAUN0015 produced its maximum value of protein 644.86 ± 19.77 μg mL -1 , and 890 mg mL -1 of carbohydrates in the absence of nitrogen. This comparative study shows that the most efficient strain for the production of protein, carbohydrates and lipids in diazotrophic conditions corresponded to Nostoc LAUN0015. However, Anabaena sp.1 and Anabaena sp.2 required high nitrogen concentrations to achieve higher values of metabolites, comparing with Nostoc strains. Nitrogen dependence for the production of pigments and high protein production in strains of Anabaena and in diazotrophic conditions for Nostoc was demonstrated. Nostoc can be cultured under nitrogen deficiency and Anabaena in sufficiency, for biomass production enriched with proteins and carbohydrates.

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Section: Discussionmentioning

confidence: 80%

COMPARATIVE GROWTH AND BIOCHEMICAL COMPOSITION OF FOUR STRAINS OF Nostoc AND Anabaena (CYANOBACTERIA, NOSTOCALES) IN RELATION TO SODIUM NITRATE

et al. 2016

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“…This quite thick (10-150 µm diameter) filamentous bacterium was surrounded by mucilage or extracellular polysaccharide (EPS). A pale brown layer (2-3 cm) was dominated by Deltaproteobacteria with subordinate Chloroflexi, Spirochaetes (Al-Thani et al, 2014), and the unicellular cyanobacterium Synechococcus; Synechococcus has been reported to thrive in hypersaline solar ponds (Roux, 1996;Madkour and Gaballah, 2012) and high temperature (60-65 o C) springs (Becraft et al, 2011). The underlying dark layers (>3 cm depth) comprise empty bacterial sheaths and deposits of orange/brown scytonemin, but more specific interpretation was not possible using only visual approaches.…”

Section: Microbiology and Palynologymentioning

confidence: 99%

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Słowakiewicz

Whitaker

Thomas

et al. 2016

Organic Geochemistry

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General rightsThis document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/pure/about/ebr-terms AbstractUnderstanding early organic matter alteration and preservation in marine carbonate-evaporite systems could improve understanding of carbon cycling and hydrocarbon source rock prediction in such environments. It is possible that organic-rich microbial mats are important contributors to preserved hydrocarbons, and to explore this we examined changes in lipid composition in such a mat from a mesohaline intertidal lagoon, eastern Qatar. The mat reaches > 5 cm thickness over a carbonate mud substrate, rich in seagrass, gastropods and other small bioclasts. Clear lamination, with distinct downward colour changes from green to pink to brown, reflects different microbial mat communities. The layers contain spheroids of probable dolomite, the precipitation of which was plausibly bacterially-mediated. Lipids [n-alkanes, fatty acids (FAs), hopanoids, isoprenoid hydrocarbons, dialkyl glycerol diethers (DAGEs), and isoprenoid (0 to 4) and branched (Ia to IIIa) GDGTs] reflect the diverse mat-building phototrophic, heterotrophic and chemoorganotrophic microorganisms, as well as some likely allochthonous material (i.e. steroids, n-alkanols, high molecular weight nalkanes). The lipids clearly document the change in microbial community, with phytene being the predominant hydrocarbon in the phototrophic surface layer. Oxygen and pH drop significantly 0.2 cm below the mat surface, coincident with the predominance of Deltaproteobacteria and increased concentrations of archaeal and bacterial glycerol dialkyl glycerol tetraether lipids andC 15 /C 16 and C 16 /C 17 dialkyl glycerol ether lipids in the deeper layers. Archaeol, likely of methanogen origin, is most abundant in the deepest layer. Allochthonous inputs occur throughout the mat, including abundant steroids, especially dinosterol and dinostanol, that are possibly related to periodic algal (dinoflagellate) blooms in the Arabian Gulf. Both n-alkanes and n-alkanols appear to be derived from seagrass. Organic matter contents decrease in the deepest mat; this suggests an overall low preservation potential of OM in intertidal mesohaline-hypersaline mats of the Arabian Gulf, which in turn suggests that these are not the major source of hydrocarbons in microbially-dominated carbonateevaporite systems.2

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“…These organisms also spread into the overlying water in some saltern systems. Massive slime formation can negatively affect the salt production process (De Medeiros Rocha & Camara 1993, Rahaman et al 1993, Roux 1996. Lack of inorganic nutrients (nitrogen, phosphorus) may induce the cells to dispose of the photosynthetically fixed carbon in the form of these extracellular polysaccharides (Roux 1996).…”

Section: Photosynthetic Processes In Benthic Gypsum Crustsmentioning

confidence: 99%

Saltern evaporation ponds as model systems for the study of primary production processes under hypersaline conditions

Oren¹

2009

Aquat. Microb. Ecol.

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Production of polysaccharide slime by microbial mats in the hypersaline environment of a Western Australian solar saltfield

Cited by 32 publications

References 25 publications

COMPARATIVE GROWTH AND BIOCHEMICAL COMPOSITION OF FOUR STRAINS OF Nostoc AND Anabaena (CYANOBACTERIA, NOSTOCALES) IN RELATION TO SODIUM NITRATE

COMPARATIVE GROWTH AND BIOCHEMICAL COMPOSITION OF FOUR STRAINS OF Nostoc AND Anabaena (CYANOBACTERIA, NOSTOCALES) IN RELATION TO SODIUM NITRATE

Biogeochemistry of intertidal microbial mats from Qatar: New insights from organic matter characterisation

Saltern evaporation ponds as model systems for the study of primary production processes under hypersaline conditions

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