Production of Polymers and Other Compounds of Industrial Importance by Cold-Adapted Yeasts

“…The structure and composition of EPSs seem to depend on the yeast strains and cultivation conditions used. Furthermore, the results of the physicochemical examination suggested that polymers synthesized by Antarctic yeasts can be used as emulsi ers, stabilizers, and thickeners within the food and cosmetic industry (Pavlova 2014).…”

Section: Introductionmentioning

confidence: 99%

Chemical characterization and biological effect of exopolysaccharides synthesized by Antarctic yeasts Cystobasidium ongulense AL101 and Leucosporidium yakuticum AL102 on murine innate immune cells

Rusinova‐Videva

Ognyanov²,

Georgiev³

et al. 2022

World J Microbiol Biotechnol

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The current study aimed to investigate exopolysaccharides (EPSs) produced by two Antarctic yeasts isolated from soil and penguin feathers samples collected on Livingston Island (Antarctica). The strains were identi ed as belonging to the species Leucosporidium yakuticum (LY) and Cystobasidium ongulense (CO) based on a molecular genetic analysis. The EPS production was investigated at submerged cultivation. Different chemical, chromatographic, and spectral analyses were employed to characterize EPSs. LY accumulated 5.5g/L biomass and 4.0g/L EPS after 120 h of cultivation, while CO synthesis 2.1g/L EPS at the end of cultivation and biomass amount reached 5.5g/L. LY-EPS was characterized by a higher total carbohydrate content (80%) and a lower protein content (18%) by comparison with CO-EPS (62%, 30%). The LY-EPS mainly consisted of mannose (90 mol%), whereas CO-EPS had also glucose, galactose, and small amounts of uronic acids (8-5 mol%). Spectral analyses (FT-IR and 1D, 2D NMR) revealed that LY-EPS comprised a typical β-(1→4)-mannan. Branched (hetero)mannan, together with β/α-glucans constituted the majority of CO-EPS. Unlike LY-EPS, which had a high percentage of high molecular weight populations, CO-EPS displayed a large quantity of lower molecular weight fractions and a higher degree of heterogeneity. LY-EPS (at a concentration of 100 ng/mL) elevated signi cantly interferon IFN-γ production in splenic murine macrophages and NK cells. The results indicated that newly identi ed EPS might affect IFN-γ signaling and in turn, might enhance anti-infectious responses. The data obtained also revealed the potential of EPSs and yeasts for practical application in biochemical engineering and biotechnology.

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“…Among these chemicals, the production of exopolysaccharides (EPS) has been emphasized 2 . The variety of EPS properties is proportional to the specificity of their molecules built up by various monomer sugars and in various structures 3 .…”

Section: Introductionmentioning

confidence: 99%

“…Biotechnology for exoglucomannan production by cold-adapted Antarctic yeast aimed to improve the component balance of pharmaceutical cosmetics has been developed 5 . The bioproduct has been purified and examined as a component of cosmetic creams and the bioreaction has been put forward as practically important 2 . Considering knowledge on cold-adapted fermentations, a bioreactor optimal performance involving Antarctic strains and its analysis, in view of bioprocess extrapolation to a larger scale, is of interest to bioengineering.…”

Section: Introductionmentioning

confidence: 99%

Agitation Effects and Kinetic Constants of Exoglucomannan Production by Antarctic Yeast Strain in a Stirred Tank Bioreactor

Vlaev

Pavlova

Rusinova‐Videva

et al. 2017

Chem.Biochem.Eng.Q.

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Exoglucomannan production by Antarctic yeast Sporobolomyces salmonicolor AL 1 is studied at semi-tech scale in a 5 L stirred tank bioreactor and the bioreaction kinetics is quantified. The organism's unconventional response to agitation is analyzed in terms of the agitation-induced mechanical stress. The yeast maximum production activity was observed at agitation rate 400 rpm and conserved or decreased at further increase in mixing intensity. Referring to the relationship of cell growth and aeration intensity, the various production activity, oxygen availability and cell growth are considered as a starting point to elucidate the possible reasons for the anomaly. At suspicion of shear detrimental effect on the yeast cells, the hydrodynamic stress acting on cell particles is determined and the microorganism morphology at low and high mixing intensity is examined. Biological stability is registered and the agitation effect is attributed to depressed metabolic activity at the evolving dissolved oxygen tension rather than to direct effect of hydrodynamics. A kinetic model is proposed. The specific growth rate (µ, h -1 ) and growth-associated (g EPS g -1 cells), and non-growth associated (g EPS g -1 cells h -1 ) production constants are determined and compared with reported estimates for similar reference EPS fermentations. The model and its parameters are determined in well-mixed cultures and could be upgraded further to account for mixing non-ideality and mass transfer in larger vessels.

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Production of Polymers and Other Compounds of Industrial Importance by Cold-Adapted Yeasts

Cited by 5 publications

References 76 publications

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

Fungal exopolysaccharides: Properties, sources, modifications, and biomedical applications

Chemical characterization and biological effect of exopolysaccharides synthesized by Antarctic yeasts Cystobasidium ongulense AL101 and Leucosporidium yakuticum AL102 on murine innate immune cells

Agitation Effects and Kinetic Constants of Exoglucomannan Production by Antarctic Yeast Strain in a Stirred Tank Bioreactor

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