Production of Polyhydroxyalkanoates from Renewable Sources Using Bacteria

Sathya, Arunachalam Bose; Sivasubramanian, V.; Santhiagu, A.; Sebastian, C.; Sivashankar, R.

doi:10.1007/s10924-018-1259-7

Cited by 45 publications

(23 citation statements)

References 68 publications

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“…According to Brojanigo et al, waste comes from various sources, including agricultural waste (crops, orchards, lignin, cellulose, and hemicellulose), industrial waste (fabrication, manufacturing, industrial processes and building sites), municipal waste (household waste, landscaping, and wastewater treatment plants), institutional waste (government centres, schools, and hospitals), and commercial waste (restaurants, stores, and markets) [ 11 ]. Sathya et al have described that most of these agricultural wastes are obtained from major commodity crops in large quantities [ 12 ]. Moreover, food processing and agricultural industries throughout the years can be good renewable feedstock for bioplastic production ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Vigneswari

Noor

Amelia

et al. 2021

Life

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Polyhydroxyalkanoates (PHA) are biodegradable polymers that are considered able to replace synthetic plastic because their biochemical characteristics are in some cases the same as other biodegradable polymers. However, due to the disadvantages of costly and non-renewable carbon sources, the production of PHA has been lower in the industrial sector against conventional plastics. At the same time, first-generation sugar-based cultivated feedstocks as substrates for PHA production threatens food security and considerably require other resources such as land and energy. Therefore, attempts have been made in pursuit of suitable sustainable and affordable sources of carbon to reduce production costs. Thus, in this review, we highlight utilising waste lignocellulosic feedstocks (LF) as a renewable and inexpensive carbon source to produce PHA. These waste feedstocks, second-generation plant lignocellulosic biomass, such as maize stoves, dedicated energy crops, rice straws, wood chips, are commonly available renewable biomass sources with a steady supply of about 150 billion tonnes per year of global yield. The generation of PHA from lignocellulose is still in its infancy, hence more screening of lignocellulosic materials and improvements in downstream processing and substrate pre-treatment are needed in the future to further advance the biopolymer sector.

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Section: Introductionmentioning

confidence: 99%

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Vigneswari

Noor

Amelia

et al. 2021

Life

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“…PHA are polymers synthesized entirely by a biological process within the cytoplasm as inclusion bodies (Sathya et al, 2018) and are naturally occurring aliphatic polyesters produced by more than 75 genera of gram positive and gram negative bacteria. PHAs are produced by microbial fermentation of carbon-rich substrates (sugars, lipids and their derivatives) in an environment of low or limited nutrients like nitrogen, phosphorus, sulphur, magnesium or oxygen concentration (Kundu et al, 2014;Blunt et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

Madhusoodanan

Hariharapura

Divyashree

2021

Environ Dev Sustain

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Conventional biopolymers resembling synthetic polymers produced from microorganisms, polyhydroxyalkanoate (PHA) synthesized utilizing renewable resources have gained supreme attention recently. PHA accumulation within the microbial cell is an innate capability of bacteria to store carbon and energy when nutrient imbalance pertains. Gram positive Bacillus endophyticus capable of synthesizing PHA was focused in this study. Study focuses on the possibility of attaining high PHA yield in relation to the varying dissolved oxygen levels induced during production phase. There was a gradual increment in PHA production from 34.5 to 53.03% when cultivated in bioreactor that maintained least dissolved oxygen of 0.4 mg/L at 32 °C. The metabolic flux of organism was altered during oxygen stress brought by varying agitation rate and volume resulting in the accumulation of Nicotinamide Adenine Dinucleotide Hydrogen (NADH), which led to increase in the overall PHA production. PHA yield was found to be favored by decreasing the oxygen supply thereby inducing an oxygen stress environment. This report was the first one that was correlating the hypothesis that links PHA yield and oxygen stress condition during production phase. PHA produced was characterized by FTIR and 1HNMR spectra in which the presence of Polyhydroxybutyrate was confirmed. Graphical abstract

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“…In fact, raw material accounts for 30–40% of the total cost of PHB; therefore, using renewable raw materials is fundamental to obtain profitable and environmentally friendly processes. Raw materials may include waste material from other industrial processes (Castilho et al ., ; Sathya et al ., ), such as whey.…”

Section: Introductionmentioning

confidence: 99%

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

Bustamante

Segarra

Tortajada

et al. 2019

Microbial Biotechnology

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Summary Polyhydroxyalkanoates ( PHA s) are polyesters of microbial origin that can be synthesized by prokaryotes from noble sugars or lipids and from complex renewable substrates. They are an attractive alternative to conventional plastics because they are biodegradable and can be produced from renewable resources, such as the surplus of whey from dairy companies. After an in silico screening to search for ß‐galactosidase and PHA polymerase genes, several bacteria were identified as potential PHA producers from whey based on their ability to hydrolyse lactose. Among them, Caulobacter segnis DSM 29236 was selected as a suitable strain to develop a process for whey surplus valorization. This microorganism accumulated 31.5% of cell dry weight ( CDW ) of poly(3‐hydroxybutyrate) ( PHB ) with a titre of 1.5 g l −1 in batch assays. Moreover, the strain accumulated 37% of CDW of PHB and 9.3 g l −1 in fed‐batch mode of operation. This study reveals this species as a PHA producer and experimentally validates the in silico bioprospecting strategy for selecting microorganisms for waste re‐valorization.

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Production of Polyhydroxyalkanoates from Renewable Sources Using Bacteria

Cited by 45 publications

References 68 publications

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Recent Advances in the Biosynthesis of Polyhydroxyalkanoates from Lignocellulosic Feedstocks

Dissolved oxygen as a propulsive parameter for polyhydroxyalkanoate production using Bacillus endophyticus cultures

In silico prospection of microorganisms to produce polyhydroxyalkanoate from whey: Caulobacter segnis DSM 29236 as a suitable industrial strain

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