Production of polyhydroxyalkanoates by Ralstonia eutropha from volatile fatty acids

Yun, Jaesuk; Sawant, Shailesh S.; Kim, Beom Soo

doi:10.1007/s11814-013-0190-9

Cited by 26 publications

(23 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, it was observed that Alcaligenes eutrophus NCIMB 11599 produced a higher P(3HB-co-3HV) copolymer yield and 3HV fraction of the copolymer in a two-stage fed-batch cultivation when using valeric acid than when using propionic acid. Additionally, Ralstonia eutropha KCTC 2658 has been identified as a PHAs copolymer producer when cultured on a mixture of acetic, propionic, and butyric acid [61]. It was reported that P(3HB-co-3HV) production reached 50% CDW using 5 g/L of acetic-acid:propionic-acid:butyric-acid at a proportion of 1:2:2.…”

Section: Synthesis Of Phas From Vfas By Pure Bacterial Culturesmentioning

confidence: 99%

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

et al. 2021

View full text Add to dashboard Cite

Waste of industrial origin produced from synthetic materials are a serious threat to the natural environment. The ending resources of fossil raw materials and increasingly restrictive legal standards for the management of plastic waste have led to research on the use of biopolymers, which, due to their properties, may be an ecological alternative to currently used petrochemical polymers. Polyhydroxyalkanoates (PHAs) have gained much attention in recent years as the next generation of environmentally friendly materials. Currently, a lot of research is being done to reduce the costs of the biological process of PHA synthesis, which is the main factor limiting the production of PHAs on the industrial scale. The volatile fatty acids (VFAs) produced by anaerobic digestion from organic industrial and food waste, and various types of wastewater could be suitable carbon sources for PHA production. Thus, reusing the organic waste, while reducing the future fossil fuel, originated from plastic waste. PHA production from VFAs seem to be a good approach since VFAs composition determines the constituents of PHAs polymer and is of great influence on its properties. In order to reduce the overall costs of PHA production to a more reasonable level, it will be necessary to design a bioprocess that maximizes VFAs production, which will be beneficial for the PHA synthesis. Additionally, a very important factor that affects the profitable production of PHAs from VFAs is the selection of a microbial producer that will effectively synthesize the desired bioproduct. PHA production from VFAs has gained significant interest since VFAs composition determines the constituents of PHA polymer. Thus far, the conversion of VFAs into PHAs using pure bacterial cultures has received little attention, and the majority of studies have used mixed microbial communities for this purpose. This review discusses the current state of knowledge on PHAs synthesized by microorganisms cultured on VFAs.

show abstract

Section: Synthesis Of Phas From Vfas By Pure Bacterial Culturesmentioning

confidence: 99%

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The production of this strain was high compared with other producing bacteria (Reddy et al 2003, Gao et al 2013, Singh et al 2013, Yun et al 2013. Its PHAs accumulation started at the end of the exponential growth and continued during the stationary phase, (Gumel et al 2013 Fig.…”

Section: S Rrna Sequence Analysismentioning

confidence: 84%

“…The best PHAs producer obtained in this study was the strain S15 that was identified as Bacillus megaterium. Particularly, many PHAs producers can accumulate a high number of granules in cytoplasm such as, Alcaligenes, Azotobacter, Bacillus, Pseudomonas, Enterobacter, Necator, Rhodobacter, Aeromonas, Ralstonia and Cupriavidus (Reddy et al 2003, Gao et al 2013, Singh et al 2013, Yun et al 2013.…”

Section: S Rrna Sequence Analysismentioning

confidence: 99%

EVALUATION OF POLYHYDROXYALKANOATES PRODUCING BACTERIA ISOLATED FROM SOILS WITH WASTES OF Cocos nucifera

Romero‐Ramírez

Hernández-Flores²,

Barrera

et al. 2017

Rev. Int. Contam. Ambie.

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHAs) are polyesters produced and accumulated in prokaryotes as carbon and energy storage materials. These polymers can be used to produce biodegradable plastics. Actually, the industrial production of PHAs for the manufacture of biodegradable plastics has a high production cost due to the use of expensive carbon substrates. An alternative to reduce these costs is the use of wastes from renewable resources. Coconut biomass, which is the most abundant agroindustrial waste in the state of Guerrero, Mexico, has a promising potential as a primary or secondary feedstock for PHAs production. In this research, it is identified and evaluated the PHAs producing bacteria isolated from soil where Cocos nucifera waste and fiber in decomposition are accumulated. It was found six bacteria capable of producing PHAs in different concentrations (0.06 to 0.422 g/L). These strains belong to the genus Staphylococcus and Bacillus. The production of PHAs of Bacillus megaterium isolate S15 was characterized, and turned out to be the best PHAs accumulating bacterium. A bank of strains capable of producing polyhydroxyalkanoates was obtained for future studies. 116 RESUMENLos polihidroxialcanoatos (PHA) son poliésteres que se producen y acumulan como materiales de almacenamiento de carbono y energía en procariotas. Sin embargo, éstos pueden emplearse para producir plásticos biodegradables. Actualmente, la producción industrial de PHA para la manufacturación de plásticos biodegradables tiene un alto costo de producción debido al uso de sustratos de carbón caros. Una alternativa para reducir los costos es utilizando residuos como sustratos a partir de recursos renovables. La fibra de coco, que es uno de los residuos agroindustriales más abundantes en el estado de Guerrero, México, tiene un prometedor potencial como materia prima principal o secundaria para la producción de PHA. En esta investigación se identifica y evalúa a bacterias productoras de PHA aisladas de suelo donde residuos de Cocos nucifera se encuentran acumulados y en descomposición. Se aislaron seis cepas capaces de producir PHA en diferentes concentraciones (0.06-0.422 g/L). Se identificaron las cepas, mismas que pertenecen a los géneros de Staphylococcus y Bacillus. Se caracterizó la producción de PHA en Bacillus megaterium S15, la cual fue la mejor cepa para acumular este poliéster. Adicionalmente, se obtuvo un banco de cepas capaces de producir PHA para estudios futuros.

show abstract

“…The results suggested that this strain showed the ability to synthesize a copolymer of P(3HB-co-3HV) under optimized conditions. It was suggested that the quantity of VFAs to co-substrates directly influences microbial cell growth, PHA content, and biopolymer composition [41]. Similarly, some investigators have shown that employing valerate as a co-substrate with acetate can produce effective copolymers with a higher contribution of HV of approximately 84.77 mole% [42].…”

Section: Vfas As a Potential And Inexpensive Substrate For Pha Productionmentioning

confidence: 99%

An Overview of Recent Advancements in Microbial Polyhydroxyalkanoates (PHA) Production from Dark Fermentation Acidogenic Effluents: A Path to an Integrated Bio-Refinery

et al. 2021

View full text Add to dashboard Cite

Global energy consumption has been increasing in tandem with economic growth motivating researchers to focus on renewable energy sources. Dark fermentative hydrogen synthesis utilizing various biomass resources is a promising, less costly, and less energy-intensive bioprocess relative to other biohydrogen production routes. The generated acidogenic dark fermentative effluent [e.g., volatile fatty acids (VFAs)] has potential as a reliable and sustainable carbon substrate for polyhydroxyalkanoate (PHA) synthesis. PHA, an important alternative to petrochemical based polymers has attracted interest recently, owing to its biodegradability and biocompatibility. This review illustrates methods for the conversion of acidogenic effluents (VFAs), such as acetate, butyrate, propionate, lactate, valerate, and mixtures of VFAs, into the value-added compound PHA. In addition, the review provides a comprehensive update on research progress of VFAs to PHA conversion and related enhancement techniques including optimization of operational parameters, fermentation strategies, and genetic engineering approaches. Finally, potential bottlenecks and future directions for the conversion of VFAs to PHA are outlined. This review offers insights to researchers on an integrated biorefinery route for sustainable and cost-effective bioplastics production.

show abstract

Production of polyhydroxyalkanoates by Ralstonia eutropha from volatile fatty acids

Cited by 26 publications

References 15 publications

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production

EVALUATION OF POLYHYDROXYALKANOATES PRODUCING BACTERIA ISOLATED FROM SOILS WITH WASTES OF Cocos nucifera

An Overview of Recent Advancements in Microbial Polyhydroxyalkanoates (PHA) Production from Dark Fermentation Acidogenic Effluents: A Path to an Integrated Bio-Refinery

Contact Info

Product

Resources

About