Production of poly-β-hydroxybutyrate (PHB) by Methylobacterium organophilum isolated from a methanotrophic consortium in a two-phase partition bioreactor

Zuñiga, Cristal; Morales, Marcia; Borgne, Sylvie Le; Revah, Sergio

doi:10.1016/j.jhazmat.2011.04.011

Cited by 62 publications

(19 citation statements)

References 36 publications

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“…Up to 67% of PHB can be theoretically produced (Asenjo and Suk, 1986). PHB yields using pure methanotrophic cultures, such us Methylobacterium organophilum or Methylocystis sp., range between 28 and 57% by dry cell depending on the accumulation conditions used (i.e., nutrient limitation conditions) (Weiland, 2006; Zuniga et al, 2011). The use of mixed cultures has focused mainly on synthetic mixtures of methanotrophs under aseptic conditions, with PHB yields of 25% by dry cell under nitrogen limiting conditions (Pieja et al, 2012) and 33% under potassium limiting conditions (Helm et al, 2008).…”

Section: Resource Recovery For a Circular Economymentioning

confidence: 99%

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

et al. 2017

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Limits in resource availability are driving a change in current societal production systems, changing the focus from residues treatment, such as wastewater treatment, toward resource recovery. Biotechnological processes offer an economic and versatile way to concentrate and transform resources from waste/wastewater into valuable products, which is a prerequisite for the technological development of a cradle-to-cradle bio-based economy. This review identifies emerging technologies that enable resource recovery across the wastewater treatment cycle. As such, bioenergy in the form of biohydrogen (by photo and dark fermentation processes) and biogas (during anaerobic digestion processes) have been classic targets, whereby, direct transformation of lipidic biomass into biodiesel also gained attention. This concept is similar to previous biofuel concepts, but more sustainable, as third generation biofuels and other resources can be produced from waste biomass. The production of high value biopolymers (e.g., for bioplastics manufacturing) from organic acids, hydrogen, and methane is another option for carbon recovery. The recovery of carbon and nutrients can be achieved by organic fertilizer production, or single cell protein generation (depending on the source) which may be utilized as feed, feed additives, next generation fertilizers, or even as probiotics. Additionlly, chemical oxidation-reduction and bioelectrochemical systems can recover inorganics or synthesize organic products beyond the natural microbial metabolism. Anticipating the next generation of wastewater treatment plants driven by biological recovery technologies, this review is focused on the generation and re-synthesis of energetic resources and key resources to be recycled as raw materials in a cradle-to-cradle economy concept.

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Section: Resource Recovery For a Circular Economymentioning

confidence: 99%

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

et al. 2017

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“…Many technoeconomic studies have demonstrated that the use of methane for PHA production can significantly reduce raw material cost, minimize environmental impact, and make PHA more attractive in economic perspectives (Rostkowski et al 2012;Levett et al 2016;Koller et al 2017). To date, many methane-utilizing cultures capable of PHA production have been reported in literature (Pfluger et al 2011;Pieja et al 2011aPieja et al , b, 2012Stein et al 2011;Zuniga et al 2011;Belova et al 2013;Matsen et al 2013;Rostkowski et al 2013;Yang et al 2013;Myung et al 2015;Sundstrom and Criddle 2015;Criddle and Myung 2016;Myung et al 2016Myung et al , 2017. However, in order to make PHA production more competitive also in economic terms, open mixed microbial cultures can be offered as an alternative strategy for producing PHAs at lower costs (Broholm et al 1992;Beun et al 2002;Salehizadeh and Van Loosdrecht 2004;Dias et al 2006;Helm et al 2006Helm et al , 2008Albuquerque et al 2010;Arcos-Hernandez et al 2010;Colombo et al 2017;Luangthongkam et al 2019).…”

Section: Introductionmentioning

confidence: 99%

The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

Luangthongkam

Strong

Mahamud

et al. 2019

Bioresour. Bioprocess.

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A methanotrophic community was enriched in a semi-continuous reactor under non-aseptic conditions with methane and ammonia as carbon and nitrogen source. After a year of operation, Methylosinus sp., accounted for 80% relative abundance of the total sequences identified from potential polyhydroxyalkanoates (PHAs) producers, dominated the methane-fed enrichment. Prior to induction of PHA accumulation, cells harvested from the parent reactor contained low level of PHA at 4.0 ± 0.3 wt%. The cells were later incubated in the absence of ammonia with various combinations of methane, propionic acid, and valeric acid to induce biosynthesis of poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV). Previous studies reported that methanotrophic utilization of odd-chain fatty acids for the production of PHAs requires reducing power from methane oxidation. However, our findings demonstrated that the PHB-containing methanotrophic enrichment does not require methane availability to generate 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV)-when odd-chain fatty acids are presented. The enrichment yielded up to 14 wt% PHA with various mole fractions of 3HV monomer depending on the availability of methane and odd-fatty acids. Overall, the addition of valeric acid resulted in a higher PHA content and a higher 3HV fraction. The highest 3HV fraction (up to 65 mol%) was obtained from the methane-valeric acid experiment, which is higher than those previously reported for PHA-producing methanotrophic mixed microbial cultures.

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“…Tomei et al 7 compared the performance of a TPPB, relative to single phase operation, in which a small volume (5 %, v/v) of the beads polymer Hytrel 8206 was used to treat aqueous mixtures of 2,4-dimethylphenol and 4-nitrophenol. Zuniga et al 9 studied the biodegradation of methane, and the accumulation of poly-hydroxybutyrate using a methanotrophic consortium and an isolated strain in a TPPB. Karimi et al 10 designed a labscale bioreactor for treatment of waste gas containing benzene, toluene and xylene (BTX).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Crude Oil Biodegradation in a Two-liquid Phase Partitioning Bioreactor

Ismail

Abdulrazzak

2015

Chem. Biochem. Eng. Q.

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Production of poly-β-hydroxybutyrate (PHB) by Methylobacterium organophilum isolated from a methanotrophic consortium in a two-phase partition bioreactor

Cited by 62 publications

References 36 publications

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

The effect of methane and odd-chain fatty acids on 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) synthesis by a Methylosinus-dominated mixed culture

Enhanced Crude Oil Biodegradation in a Two-liquid Phase Partitioning Bioreactor

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