Production of phytase by Aspergillus niger in submerged and solid-state fermentation

Papagianni, Maria; Nokes, Sue E.; Filer, Keith

doi:10.1016/s0032-9592(99)00088-6

Cited by 64 publications

(38 citation statements)

References 18 publications

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“…Among fungi examined, M. racemosus NRRL 1994 produced the best result, 26 IU phytase activity/g DM on optimized coconut press cake medium [5]. Others have reported good growth and phytase production on canola meal, and on a mixture of wheat bran and soybean meal [1,8,15,23].…”

Section: Resultsmentioning

confidence: 98%

“…By using an assumed fermentation cost of US $50/MT substrate (based on comparison with the costs of established SSF processes, composting about US $6/MT, ensiling about US $10/MT, malting about US $20/MT), the estimated cost could be an affordable US $1.00/MT feed. In the literature, phytase production by SF is in the range 0.1-15 IU/ml [7,12,23], corresponding to 2.0-300 IU/g DM substrate in a 5% slurry. These data are subject to a large degree of uncertainty, because different authors use different techniques for phytase determination.…”

Section: Optimization Of Medium Composition For Ssf By a Ficuummentioning

confidence: 99%

“…Phytase may be produced directly in SSF by filamentous fungi on selected feed ingredients, and the crude product may be mixed in feed rations as a value-added supplement. The fungal product contains not only phytase, but also accessory enzymes, cellulases, hemicellulases, pectinases, amylases, fungal protein and organic acids that increase feed digestibility and access to phytin in plant cells [8,9,20,22,23]. Phytase production by SSF is described in the literature without any rigorous studies on optimizing fermentation conditions for maximal phytase production [8,9,20,21,23,26,27].…”

Section: Introductionmentioning

confidence: 99%

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Optimization of phytase production by solid substrate fermentation

Bogar

Szakács

Linden

et al. 2003

J IND MICROBIOL BIOTECHNOL

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The production of phytase by three feed-grade filamentous fungi ( Aspergillus ficuum NRRL 3135, Mucor racemosus NRRL 1994 and Rhizopus oligosporus NRRL 5905) on four commonly used natural feed ingredients (canola meal, cracked corn, soybean meal, wheat bran) was studied in solid substrate fermentation (SSF). A. ficuum NRRL 3135 had the highest yield [15 IU phytase activity/g dry matter (DM)] on wheat bran. By optimizing the supplementation of wheat bran with starch and (NH(4))(2)SO(4), phytase production increased to 25 IU/g DM. Optimization was carried out by Plackett-Burman and central composite experimental designs. Using optimized medium, phytase, phosphatase, alpha-amylase and xylanase production by A. ficuum NRRL 3135 was studied in Erlenmeyer flask and tray SSF. By scaling up SSF from flasks to stationary trays, activities of 20 IU phytase activity/g DM were reproducibly obtained.

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Section: Resultsmentioning

confidence: 98%

Section: Optimization Of Medium Composition For Ssf By a Ficuummentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of phytase production by solid substrate fermentation

Bogar

Szakács

Linden

et al. 2003

J IND MICROBIOL BIOTECHNOL

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“…Other researchers also reported that 100 lm is a limitation distance for mycelium which with biofilm [39]. Investigations have shown that pellet formation is necessary for the production of some enzymes from filamentous microorganisms, such as polygalacturonase [14,37], phytase [16], glucoamylase [8], fructosyltransferase [15], and other proteins [33]. Based on other researchers and their results, Papagianni concluded that such phenomena may be related to diffusional limitations in pellets, which either reduce the extent of catabolic repression in pellets or limit the oxygen supply, preventing oxidative inactivation of a specific set of enzymes [1,8].…”

Section: Morphology and Broth Rheology Study In Strmentioning

confidence: 99%

“…ElEnshasy [10] reported that culture conditions resulting in the formation of smaller fungal pellets with an increased mycelial density result in higher yields of extracellular glucose oxidase for recombinant A. niger. Other enzymes production were also affected by the change of mycelial morphology, they were amylase [11], lipase [12,13], polygalacturonase [14], neo-fructosyltransferase [15], and phytase [16]. The relation between fungal morphology and process productivities has attracted interest from both academic and industry and attempts have been made to manipulate morphology to achieve maximal performance.…”

Section: Introductionmentioning

confidence: 99%

Changes in morphology of Rhizopus chinensis in submerged fermentation and their effect on production of mycelium-bound lipase

Teng

Wang

2008

Bioprocess Biosyst Eng

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In order to control suitable mycelium morphology to obtain high lipase productivity by Rhizopus chinensis in submerged fermentation, the effects of fungal morphology on the lipase production by this strain both in shake flask and fermentor were investigated. Different inoculum level and shear stress were used to develop distinctive morphologies. Analyses and investigations both on micromorphology and macromorphology were performed. Study of micromorphology reveals that micromorphologies for dispersed mycelia and aggregated mycelia are different in cell shape, biosynthetic activity. Macromorphology and broth rheology study in fermentor indicate that pellet formation results in low broth viscosity. Under this condition, the oil can disperse sufficiently in broth which is very important for lipase production. These results indicate that morphology changes affected the lipase production significantly for R. chinensis and the aggregated mycelia were suggested to achieve high lipase production.

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Lipase production and Penicillium simplicissimum morphology in solid‐state and submerged fermentations

Gutarra

Godoy

Silva

et al. 2009

Biotechnology Journal

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A comparative study of Penicillium simplicissimum morphology and lipase production was performed using solid-state (SSF) and submerged (SmF) fermentation. SSF was carried out on babassu cake as culture medium and SmF on a semi-synthetic medium and a medium based on suspended babassu cake grains. Yield of product on biomass, specific activity and conidia production were 3.3-, 1.3- and 2-fold higher in SSF. In SmF, the type of fungus growth differed according to the medium. Using the semi-synthetic medium, the fungus formed densely interwoven mycelial masses without conidia production, whereas using the babassu-based medium the fungus formed free mycelia and adhered to the surfaces of the grains, producing conidia. The results show that babassu cake induces conidiation in SmF. In SSF, the fungus not only grew on the surface of the grains, producing conidia abundantly, but also effectively colonized and penetrated the babassu particles. The high conidia production and lipase productivity in SSF may be related to the low availability of nutrients or to other stimuli associated with this type of fermentation. Thus, the high production of the thermostable P. simplicissimum lipase, using a non-supplemented, low-cost agro-industrial residue as the culture medium, demonstrates the biotechnological potential of SSF for the production of industrial enzymes.

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Production of phytase by Aspergillus niger in submerged and solid-state fermentation

Cited by 64 publications

References 18 publications

Optimization of phytase production by solid substrate fermentation

Optimization of phytase production by solid substrate fermentation

Changes in morphology of Rhizopus chinensis in submerged fermentation and their effect on production of mycelium-bound lipase

Lipase production and Penicillium simplicissimum morphology in solid‐state and submerged fermentations

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