Production of phycobiliproteins, bioplastics and lipids by the cyanobacteria Synechocystis sp. treating secondary effluent in a biorefinery approach

Senatore, Vincenzo; Rueda, Estel; Català, Marta Bellver; Díez-Montero, Rubén; Ferrer, Ivet; Zarra, Tiziano; Naddeo, Vincenzo; García, Jesús Miguel Bairán

doi:10.1016/j.scitotenv.2022.159343

Cited by 19 publications

(7 citation statements)

References 35 publications

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“…Tris-HCl pH 8 did not affect the purity throughout the FT cycles. The maximum purity, reported in the literature, of the extracted PC from Synechocystis sp., obtained by using 0.1 M phosphate buffer (pH 7) after one FT cycle, was 0.7 [ 42 ]. In the case of N. commune , the purity ratio of PC, using 1 M Tris-HCl buffer (pH 8), decreased during all the FT cycles, while the opposite occurred for O. okeni [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

Optimizing Phycocyanin Extraction from Cyanobacterial Biomass: A Comparative Study of Freeze–Thaw Cycling with Various Solvents

Pispas,

Manthos,

Sventzouri

et al. 2024

Marine Drugs

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Cyanobacterial phycocyanin pigment is widely utilized for its properties in various industries, including food, cosmetics, and pharmaceuticals. Despite its potential, challenges exist, such as extraction methods impacting yield, stability, and purity. This study investigates the impact of the number of freeze–thaw (FT) cycles on the extraction of phycocyanin from the wet biomass of four cyanobacteria species (Arthrospira platensis, Chlorogloeopsis fritschii, Phormidium sp., and Synechocystis sp.), along with the impact of five extraction solutions (Tris-HCl buffer, phosphate buffer, CaCl2, deionized water, and tap water) at various pH values. Synechocystis sp. exhibited the highest phycocyanin content among the studied species. For A. platensis, Tris-HCl buffer yielded maximum phycocyanin concentration from the first FT cycle, while phosphate buffer provided satisfactory results from the second cycle. Similarly, Tris-HCl buffer showed promising results for C. fritschii (68.5% of the maximum from the first cycle), with the highest concentration (~12% w/w) achieved during the seventh cycle, using phosphate buffer. Phormidium sp. yielded the maximum pigment concentration from the first cycle using tap water. Among species-specific optimal extraction solutions, Tris-HCl buffer demonstrated sufficient extraction efficacy for all species, from the first cycle. This study represents an initial step toward establishing a universal extraction method for phycocyanin from diverse cyanobacteria species.

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Section: Resultsmentioning

confidence: 99%

Optimizing Phycocyanin Extraction from Cyanobacterial Biomass: A Comparative Study of Freeze–Thaw Cycling with Various Solvents

Pispas,

Manthos,

Sventzouri

et al. 2024

Marine Drugs

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“… 27 3.83 ± 0.24 [ 168 ] Synechocystis sp. Secondary effluent 10 L pH 7.99; conductivity 2.47 ± 0.08 mS.cm − 1 ; COD 96.65 ± 40.70 mgO 2 .L − 1 Hydraulic retention time (HRT) 8 d; lipid accumulation 44.7% (30 d) 4.8 - [ 169 ] Bacillus sp. CYR1 Cheese whey + raw sewage (Rantoh wastewater treatment plant, Japan) 500 mL 4% Bacillus sp.…”

Section: Bioprocess Parameter Affect and Scaleup Studiesmentioning

confidence: 99%

A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater

Ahuja,

Singh,

Mahata

et al. 2024

Microb Cell Fact

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Background Plastic is widely utilized in packaging, frameworks, and as coverings material. Its overconsumption and slow degradation, pose threats to ecosystems due to its toxic effects. While polyhydroxyalkanoates (PHA) offer a sustainable alternative to petroleum-based plastics, their production costs present significant obstacles to global adoption. On the other side, a multitude of household and industrial activities generate substantial volumes of wastewater containing both organic and inorganic contaminants. This not only poses a threat to ecosystems but also presents opportunities to get benefits from the circular economy. Main body of abstract Production of bioplastics may be improved by using the nutrients and minerals in wastewater as a feedstock for microbial fermentation. Strategies like feast-famine culture, mixed-consortia culture, and integrated processes have been developed for PHA production from highly polluted wastewater with high organic loads. Various process parameters like organic loading rate, organic content (volatile fatty acids), dissolved oxygen, operating pH, and temperature also have critical roles in PHA accumulation in microbial biomass. Research advances are also going on in downstream and recovery of PHA utilizing a combination of physical and chemical (halogenated solvents, surfactants, green solvents) methods. This review highlights recent developments in upcycling wastewater resources into PHA, encompassing various production strategies, downstream processing methodologies, and techno-economic analyses. Short conclusion Organic carbon and nitrogen present in wastewater offer a promising, cost-effective source for producing bioplastic. Previous attempts have focused on enhancing productivity through optimizing culture systems and growth conditions. However, despite technological progress, significant challenges persist, such as low productivity, intricate downstream processing, scalability issues, and the properties of resulting PHA. Graphical abstract

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“…Regarding pigments, Senatore et al have reported an integrated biorefinery-based procedure to obtain phycobiliproteins, poly-(hydroxybutyrate) (PHB), and lipids. 29 Likewise, Martins et al have reported the extraction and fractionation of pigments such as chlorophyll and fucoxanthin from Saccharina latissima, using a phosphonium-based IL surfactant combined with sunflower oil and water. 30 However, the residual biomass after pigment extraction was not used in both cases.…”

Section: Introductionmentioning

confidence: 99%

“…Most biorefinery schemes have been focused on lipid extraction and further biodiesel production. , Biorefinery schemes are scarce for the rest of the bioproducts (proteins, carbohydrates, pigments). Regarding pigments, Senatore et al have reported an integrated biorefinery-based procedure to obtain phycobiliproteins, poly(hydroxybutyrate) (PHB), and lipids . Likewise, Martins et al have reported the extraction and fractionation of pigments such as chlorophyll and fucoxanthin from Saccharina latissima, using a phosphonium-based IL surfactant combined with sunflower oil and water .…”

Section: Introductionmentioning

confidence: 99%

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

Sánchez-Laso

Espada

Rodríguez

et al. 2023

Ind. Eng. Chem. Res.

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A new biorefinery from Arthrospira platensis was proposed to obtain phycocyanin (PC) and a biocrude by hydrothermal liquefaction (HTL). PC is a high-added-value phycobiliprotein widely used as a food colorant and in the nutraceutical and pharmaceutical industries. However, the use of conventional solvents in the extraction process and the purity grade of the extract are shortcomings in bioproduct production. PC was extracted using a reusable ionic liquid [EMIM][EtSO4], achieving a PC purity of the lowest commercial grade. Therefore, two downstream processes were applied: (1) dialysis + precipitation and (2) aqueous two-phase system (ATPS) + dialysis + precipitation. After the second purification process, the PC purity increased remarkably to reach the analytical grade for pharmaceutical and nutraceutical applications. The waste biomass (WB) obtained in the PC extraction was valorized by hydrothermal liquefaction (HTL) to produce a biocrude. The biocrude yield and composition remarkably enhanced using isopropanol at 350 °C as a cosolvent.

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Production of phycobiliproteins, bioplastics and lipids by the cyanobacteria Synechocystis sp. treating secondary effluent in a biorefinery approach

Cited by 19 publications

References 35 publications

Optimizing Phycocyanin Extraction from Cyanobacterial Biomass: A Comparative Study of Freeze–Thaw Cycling with Various Solvents

Optimizing Phycocyanin Extraction from Cyanobacterial Biomass: A Comparative Study of Freeze–Thaw Cycling with Various Solvents

A review on microbes mediated resource recovery and bioplastic (polyhydroxyalkanoates) production from wastewater

Novel Biorefinery Approach for Phycocyanin Extraction and Purification and Biocrude Production from Arthrospira platensis

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