Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos

Rosenberg, Urs B.; Preiss, Anette; Seifert, E; Jäckle, Herbert; Knipple, Douglas C.

doi:10.1038/313703a0

Cited by 201 publications

(72 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the mid-1980s, several studies reported that transgenic antisense RNA could inhibit gene expression in Drosophila, mammalian cells, and plants [1][2][3][4]. This phenomenon was termed post-transcriptional gene silencing in the plant biology world.…”

Section: Introductionmentioning

confidence: 99%

Small RNA Regulators of T Cell-Mediated Autoimmunity

Jeker

Bluestone²

2010

J Clin Immunol

View full text Add to dashboard Cite

Background MicroRNAs (miRNAs) are short singlestranded RNA molecules that regulate gene expression post-transcriptionally. Several hundred miRNAs exist in the mammalian genome and regulate developmental processes, cell cycle, and survival. Methods In this review, we highlight general modes of miRNA function and relate them to how such regulation can be beneficial for immune homeostasis and the prevention of autoimmune diseases. We highlight examples of experimentally verified miRNA function and their target genes in the immune system and place them in context of concepts relevant to an understanding of autoimmune pathogenesis. Where available, we refer to clinical correlations. Finally, we speculate how emerging knowledge about miRNA function in the immune system might be used diagnostically and therapeutically.

show abstract

Section: Introductionmentioning

confidence: 99%

Small RNA Regulators of T Cell-Mediated Autoimmunity

Jeker

Bluestone²

2010

J Clin Immunol

View full text Add to dashboard Cite

show abstract

“…Recently, results of a series of experiments (8,9,25,26,28,35,45) have shown that introduction of an antisense RNA complementary to a specific mRNA into cells can effectively create or mimic the null-mutant phenotype, which should be quite useful for investigating gene products of unknown physiological function. Although the exact molecular mechanism of antisense RNA inhibition of specific gene expression is not known, it has been assumed that the formation of a stable RNA-RNA hybrid in vivo results in inhibition of RNA transport, block of translation, or an increased rate of RNA turnover (25).…”

mentioning

confidence: 99%

Antisense RNA of proto-oncogene c-fos blocks renewed growth of quiescent 3T3 cells.

Nishikura¹,

Murray²

1987

Mol. Cell. Biol.

359

132

View full text Add to dashboard Cite

Mouse 3T3 cells were transformed with an antisense c-fos gene fused to a mouse mammary tumor virus promoter. In transformants that integrated a large number of antisense c-fos sequences, the usual large increase in c-fos mRNA and protein following stimulation of quiescent cells by platelet-derived growth factor was blocked in the presence of dexamethasone. These cells subsequently also failed to show the stimulation of DNA synthesis normally induced by platelet-derived growth factor. Appropriate expression of c-fos appears to be a prerequisite for reentry of quiescent cells into the cell cycle.

show abstract

“…Antisense RNA transcripts have been used to generate dominant mutations in eukaryotes by inhibiting the expression of specifically targeted RNA species (1)(2)(3)(4)(5)(6)(7)(8)(9), and the technique has been applied to address fundamental questions in animal molecular biology (10)(11)(12)(13)(14)(15)(16). The antisense approach in plants, if applicable, promises to make significant contributions to elucidating the functions of cloned plant genes and identifying genes with known phenotypes for which there are no biochemical markers.…”

mentioning

confidence: 99%

A stable bifunctional antisense transcript inhibiting gene expression in transgenic plants

Delauney

Tabaeizadeh

Verma

1988

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Tobacco (20) to give pl9S-C (Fig. 1A).An 850-bp Sac I-EcoRV fragment from pUC-19S-CAT, comprising the CAT sequence and the 3' proximal 65 bp of the 19S promoter sequence (including all 12 bp of the 19S 5' untranslated region), was cloned in the antisense orientation next to the nos 3' region in a pUC18 derivative to give pACN9. A 1.95-kilobase (kb) Pvu II fragment from pAGS120 (21) containing a chimeric HPT gene was inserted downstream from the antisense CAT-nos 3' sequence in pACN9.The rbcS promoter (-1040 to + 169) from Nicotiana plumbaginifolia, isolated as a 1.2-kb EcoRI-Pvu II fragment from pNPSS8B (a gift from N.-H. Chua), was cloned into the EcoRI and Sma I sites of pBinl9 to give pBrbcS. A 3.3-kb Xba 1-HindlIl fragment containing the antisense CAT-nos 3' fusion and the chimeric HPT gene was cloned into Xba I-and HindIII-linearized pBrbcS to give pRAC-H (Fig. 1B).An EcoRI-blunted HindIII fragment from pUC13/35SCAT (22) containing the CaMV 35S promoter (-941 to + 9) was cloned into the EcoRI and Sma I sites of Bluescript M13+ (Stratagene, San Diego) to give pBS-35S. A 1300-bp Xba IHincII fragment from pACN9 containing the antisense CATnos 3' sequence was cloned into the Xba I and blunted Sac II sites of pBS-35S to give pBS-35S-AC. The HPT coding sequence, isolated as a 1070-bp Xho II fragment from pAGS120, was inserted between the 35S promoter and antisense CAT-nos 3' sequence of pBS-35S-AC to give

show abstract

Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos

Cited by 201 publications

References 17 publications

Small RNA Regulators of T Cell-Mediated Autoimmunity

Small RNA Regulators of T Cell-Mediated Autoimmunity

Antisense RNA of proto-oncogene c-fos blocks renewed growth of quiescent 3T3 cells.

A stable bifunctional antisense transcript inhibiting gene expression in transgenic plants

Contact Info

Product

Resources

About