Production of pediocin PA-1, and coproduction of nisin A and pediocin PA-1, by wild Lactococcus lactis strains of dairy origin

Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157:H7 in cheese

“…lactis CL2 is lower than that of Lc. lactis CL1 (Reviriego et al, 2004), pediocin in cheese with Lc. lactis CL2 could be below the detection level.…”

“…ıguez, Gaya, Nun˜ez, & Medina, 1999). Both strains were transformed by Reviriego et al (2004) to produce pediocin PA-1 heterologously. The objective of the present work was to evaluate the antimicrobial ability of the transformants Lc.…”

“…lactis CL2 (Nis + , Ped + ) against L. monocytogenes, S. aureus and E. coli O157:H7 in cheese. (Reviriego et al, 2004) were used as adjuncts to the starter culture in cheesemaking. They were grown in MRS broth (Biolife) at 30 C for 18 h and subcultured twice in sterile reconstituted skim milk supplemented with 0.3% yeast extract before use in cheesemaking.…”

Heterologous expression, purification and refolding of an anti-listerial peptide produced by Pediococcus acidilactici K7

“…Pediocin PA-1 has been heterologously expressed in several strains of E. coli, P. pentosaceus, Lactococcus lactis, Lactobacillus sakei and yeast. The antimicrobial activity of heterologously expressed pediocin PA-1 varied from approximately 25% less than that of the wild type (WT) to 2.5 fold higher depending on the expression system (Marugg et al 1992;Axelsson et al 1998;Coderre and Somkuti, 1999;Somkuti and Steinberg, 2003;Reviriego et al 2005). While expressing the pediocin PA-1 in LAB, it is important to know that many of the LAB are naturally sensitive to pediocin PA-1.…”

Enhanced production of pediocin PA-1 in wild nisin- and non-nisin-producing Lactococcus lactis strains of dairy origin

“…Therefore, production of pediocin PA-1 in strains of dairy origin is an attractive objective. Heterologous production of this bacteriocin by strains of Lactococcus lactis has been achieved using two strategies: introduction of the whole pediocin PA-1 operon (pedABCD) (Buyong, Kok, & Luchansky, 1998;Chikindas, Venema, Ledeboer, Venema, & Kok, 1995), or the exploitation of the amino acid homologies shared among leader peptides, and also among transporters of most class II bacteriocins (Horn et al, 1998;Horn et al, 1999;Horn, Ferna´ndez, Dodd, Gasson, & Rodrı´guez, 2004;Reviriego et al, 2005). However, the results of the studies cited above cannot be compared because of the different hosts, plasmids, promoters, substrates and antimicrobial assays employed.…”