Production of occlusion bodies of Anticarsia gemmatalis multiple nucleopolyhedrovirus in serum-free suspension cultures of the saUFL-AG-286 cell line: Influence of infection conditions and statistical optimization

Production of Insecticidal Baculoviruses in Insect Cell Cultures: Potential and Limitations

“…The supply of hydrophobic nutrients is replaced by adding microemulsions that contain a source of lipids and cholesterol (Maiorella et al, 1988;Ikonomou et al, 2001). The most used sources of lipids are the methyl esters of fatty acids isolated from the liver of marine fishes, but recently it has been described the use of cooking soybean oil, a cheaper and more abundant source of lipids (Micheloud et al, 2009). In addition, microemulsions also contain the surfactant Pluronic F68, whose presence protects the cells from the detrimental influence of bubbles in sparged bioreactors.…”

“…The composition of the LCM medium was not disclosed. On the other hand, the low-cost medium UNL-10 was developed to grow the saUFL-AG-286 cell line, useful to produce the baculovirus AgMNPV (Micheloud et al, 2009). The yields of occlusion bodies in suspension cultures, using optimized parameters of infection, were as high as 3 x 10 11 OBs L -1 , with specific yields higher than 600 OBs/cell.…”

Adaptation of the Se301 insect cell line to suspension culture. Effect of turbulence on growth and on production of nucleopolyhedrovius (SeMNPV)

“…The optimal MOI generally depends on each virusinsect cell line system (Micheloud et al 2009). Our results diverge from those in which enhanced productivities of both heterologous proteins and viruses are frequently observed at relatively low MOIs (\0.1) (Enden et al 2005).…”

Quality Control of Baculoviral Bioinsecticide Production

“…Baculoviruses are produced by infection processes in susceptible larvae or in in vitro cell cultures. First approach is appropriate and inexpensive in small-scale, but big productions prefer the use of cell bioreactors (van Beek & Davis, 2007;Micheloud et al, 2009;Mengual Gómez et al, 2010). This technology would allow the standardization of production processes and achieve bioinsecticides with reproducible quality.…”

“…Other problems are related to the composition of culture media and the availability of susceptible insect cell lines to each baculovirus. Actually, many researchers are working on the establishment of new cell lines or modifying existing ones to improve their performance, while others have focused on developing proper and cheaper formulations of growth media for cell propagation in vitro (Agathos, 2007;Micheloud et al, 2009). …”