Production of Nα-benzyloxycarbonyl-l-aminoadipic acid and Nα-benzyloxycarbonyl-d-aminoadipic acid with Rhodococcus sp. AIU Z-35-1

Enzymes responsible for metabolism of Nα-benzyloxycarbonyl-l-lysine in microorganisms

“…In this analysis, N-Z-L-AASA and N-Z-L-AAA were identified as reaction products from N-Z-L-lysine in both strains. It was therefore estimated that N-Z-L-lysine is converted to N-Z-L-AAA via N-Z-L-AASA in both strains [4,5].…”

“…Recently, simple biochemical methods for selective production of N-Z-L-AASA and N-Z-L-AAA were also reported using A. niger AKU 3302 or Rhodococcus sp. AIU Z-35-1 [3][4][5][6]. In this paper, enzymes…”

Enzymes Responsible for the Conversion of Nα‐[(Benzyloxy)carbonyl]‐D‐lysine to Nα‐[(Benzyloxy)carbonyl]‐D‐aminoadipic Acid by Rhodococcus sp. AIU Z‐35‐1

“…Rhodoccocus sp. AIU Z-35-1 was incubated in an l-lysine medium at 308 for 1 or 2 d with shaking at 120 strokes per min, according to the method of Isobe et al [3]. The cells were harvested by centrifugation at 20,000g for 10 min, washed with 0.1m potassium phosphate buffer pH 7.0, and stored at À 208 until use.…”

“…AIU Z-35-1, as a high producer of N a -Z-l-AAA from N a -Z-l-lysine, to overcome the above drawbacks, and developed an efficient method for production of N a -Z-l-AAA by the cell reaction [3]. This strain was also useful for the production of N a -Z-l-AASA, N a -Z-d-AASA, and N a -Z-d-AAA, since N a -Z-l-lysine was converted to N a -Z-l-AAA via N a -Z-l-AASA and N a -Z-d-lysine was converted to N a -Z-d-AAA via N a -Z-d-AASA [3] [4]. Recently, we identified the enzymes catalyzing the conversion of N a -Z-l-lysine to N a -Z-l-AAA [5] [6].…”

A Simple Enzymatic Method for Production of a Wide Variety of D-Amino Acids Using L-Amino Acid Oxidase from Rhodococcus sp. AIU Z-35-1

“…Recently, we isolated a new bacterial strain, Rhodococcus sp. AIU Z-35-1, for production of N α -benzyloxycarbonyl- l -aminoadipic acid ( N α -Z- l -AAA) from N α -benzyloxycarbonyl- l -lysine ( N α -Z- l -lysine) [15]. In the analysis of this reaction, it was demonstrated that N α -Z- l -lysine was converted to N α -Z- l -AAA via N α -benzyloxycarbonyl- l -aminoadipate- δ -semialdehyde ( N α -Z- l -AASA), and the conversion of N α -Z- l -lysine into N α -Z- l -AASA was catalyzed by a l -specific amino acid oxidase ( l -AAO) with broad substrate specificity [16].…”