Production of Novel Polygalacturonase from Bacillus paralicheniformis CBS32 and Application to Depolymerization of Ramie Fiber

Rahman, Md. Saifur; Choi, Yoon Seok; Kim, Young Kyun; Park, Chulhwan; Yoo, Jin Cheol

doi:10.3390/polym11091525

Cited by 21 publications

(6 citation statements)

References 33 publications

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“…Denaturation of the enzyme was due to the release of covalent and hydrogen bond in the structure of the enzyme protein. The liberation of these bonds results in the disintegration of the enzyme protein folds that caused enzyme inactivation (Reece et al, 2011) The optimum PG activity was observed with NaCl followed by CaCl2 in BR3 isolate the opposite of what is in isolate BR5 and There was no obvious difference was observed in PG activity when using NaCl and CaCl2 in BR2 isolate, while the inhibited of PG activity was observed with CO 2+ and K + ions several studies (Manachini et al, 2005;Trindade et al, 2016;Rahman et al, 2019;Almulaiky et al, 2020) were clarified that the effect of various metal ions on the activity of PG enzyme.…”

Section: Discussionmentioning

confidence: 99%

“…An endo-PG (EC 3.2.1.82), PehA (also known as PglA), cleaves the pectic polymer internally at random, releasing large oligomers, predominantly galacturonic acid (galUA) trimers (Allen et al, 1991;Roberts et al, 1988). Many investigators studied the effects of pH, temperature, incubation time, metals ions and Na concentration on the PG activity (Mathew et al, 2008;Maisuria et al, 2010;Anam and Latif., 2012;Martins et al, 2013;Rahman et al, 2019;Almulaiky et al, 2020;Berber and Çetinkaya, 2020). They assayed the enzyme activity at different pH, different temperatures, different incubation times, different metals ions and different NaCl concentrations under standard assay conditions.…”

Section: Introductionmentioning

confidence: 99%

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Relationship of Polygalacturonase Enzyme Activity between Brown Rot Isolates and their Virulence on Potato Cultivars

Eid

El-shamy

2021

Alexandria Science Exchange Journal

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Ralstonia solanacearum, the bacterium that causes bacterial wilt in many plant species, produces a number of extracellular plant cell wall-degrading enzymes that are thought to be virulence factors. Endopolygalacturonase (endo-PG), PehA, and two exo-PGs, pehB and pehC, are among them. R. solanacearum was isolated and identified from sick potato tubers, yielding five isolates (BR1, BR2, BR3, BR4, and BR5). The pathogenicity of the isolates was determined, results revealed that the isolates differed in their virulence. R. solanacearum isolates (BR3, BR2, and BR5) had different levels of virulence on the potato stems of the examined cultivar (strong, moderate, and weak) respectively, so these isolates were chosen to assess potato cultivars for susceptibility to R. solanacearum isolates. R. solanacearum susceptibility differed amongst potato varieties. Spunta cultivar was the most sensitive, whereas Nicola cultivar was the most resistant. Mondial was a fairly sensitive cultivar. The activity of the PG enzyme was measured in the five isolates. BR3, BR2 and BR5 isolates were high, moderate and low activity, respectively. The optimum PG activity was observed at pH 5 and 5.5 and PG activity reached the highest level at 2 and 2.5 from incubation time, the maximum activity of PG was observed at 30 ºC and 40 ºC of incubation temperature, the optimum PG activity was observed with NaCl followed by CaCl2, The optimum NaCl concentrations for PG activity were 0.1 and 0.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relationship of Polygalacturonase Enzyme Activity between Brown Rot Isolates and their Virulence on Potato Cultivars

Eid

El-shamy

2021

Alexandria Science Exchange Journal

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“…Ample diversity of alpha-amylases with respect to the molecular weights (10 to 210 kDa) has been reported (Mehta and Satyanarayana, 2016). Raul et al (2014) reported molecular weight of α-amylase from Bacillus species ranges between 50 and 60kDa, while Rehman et al (2019) detected Polygalacturonase from Bacillus paralicheniformis to have molecular weight of 110 kDa. Hatice et al (2016) observed two protein bands belonging to pectinase of B. subtilis with molecular weights 60 and 64 kDa.…”

Section: Molecular Identification Of Selected Bacteriamentioning

confidence: 99%

“…This peel is a rich source of carbohydrates, flavonoids and oxidants. In general, pectinases, cellulases and amylases are involved in the degradation of pectin, cellulose and starch rich substrates (fruit peels) where bacteria play a crucial role in producing these enzymes (Ahlawat et al, 2009;Rehman et al, 2019;Dai et al, 2020). Citrus peel is reported to be one of the potential substrates for production of pectinase solely.…”

Section: Introductionmentioning

confidence: 99%

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

Singh¹,

Garg²,

Mathur³

et al. 2022

JEB

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Aim: To determine feasibility of using mosambi peel as substrate for multi enzyme production through microbial intervention. Methodology: Thirty-three bacterial isolates were isolated from biodegradable organic substrates and investigated in-vitro their biodegradable activities viz. pectinolytic, cellulolytic and amylolytic. The best performing bacterial isolate, exhibiting aforesaid activities were selected and identified using 16S rRNA technique. The factors, that influenced the fermentation, viz. temperature, pH and incubation period of bacterial culture were optimized for maximum production of multi enzymes viz. pectinase, cellulase and amylase using mosambi peel as substrate under solid-state fermentation conditions. Molecular weights of different enzymes present in multi-enzyme preparation were determined by SDS PAGE. The juice extraction efficiency of crude multi enzyme preparation was compared with that of pure commercial enzyme. Results: Out of thirty-three bacterial isolates, after primary and secondary screening, the best performing bacterial isolate was identified as Bacillus subtilis strain NG 105 (Genbank accession number MN493055). Higher enzyme activities were observed at pH 7.0, incubation temperature of 35°C and incubation period of 10 days for pectinase, cellulase and amylase using mosambi peel as substrate. The purified enzymes characterized by SDS-PAGE, revealed to have molecular mass of 65 kDa for pectinase, 50 kDa for cellulase and 55 kDa for amylase. Bagasse fibre was found to be the most suited matrix for immobilization. The juice extraction efficiency of partially purified crude multienzyme preparation was 88% of commercial pectinase. Interpretation: Mosambi peel is a suitable substrate for multienzyme production using Bacillus subtilis under solid-state fermentation condition.

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“…B. paralicheniformis were reported as plant-associated bacteria, displaying growth promoting abilities (Annapurna et al, 2018) and the potential to be used as phytopathogen biocontrol agents (Wang et al, 2017;Pylro et al, 2019;Jinal et al, 2020;Ramirez-Carino et al, 2020). B. paralicheniformis strains also have the potential to be used for biotechnological purposes and for bioremediation (Rahman et al, 2019;Khan et al, 2018;SanthaKalaikumari et al, 2021;Ganesh Kumar et al, 2021). Ahire et al (2020) reported the production of a bacitracin, an antimicrobial peptide active against a range of Gram-positive bacteria, by strains of B. paralicheniformis.…”

Section: Body Of Knowledgementioning

confidence: 99%

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 14: suitability of taxonomic units notified to EFSA until March 2021

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2021

EFS2

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The qualified presumption of safety (QPS) approach was developed to provide a regularly updated generic pre-evaluation of the safety of biological agents, intended for addition to food or feed, to support the work of EFSA's Scientific Panels. The QPS approach is based on an assessment of published data for each agent, with respect to its taxonomic identity, the body of relevant knowledge, safety concerns and occurrence of antimicrobial resistance. Safety concerns identified for a taxonomic unit (TU) are, where possible, confirmed at the species/strain or product level and reflected by 'qualifications'. In the period covered by this statement, no new information was found that would change the status of previously recommended QPS TUs. Schizochytrium limacinum, which is a synonym for Aurantiochytrium limacinum, was added to the QPS list. Of the 78 microorganisms notified to EFSA between October 2020 and March 2021, 71 were excluded; 16 filamentous fungi, 1 Dyella spp., 1 Enterococcus faecium, 7 Escherichia coli, 1 Streptomyces spp., 1 Schizochytrium spp. and 44 TUs that had been previously evaluated. Seven TUs were evaluated: Corynebacterium stationis and Kodamaea ohmeri were re-assessed because an update was requested for the current mandate. Anoxybacillus caldiproteolyticus, Bacillus paralicheniformis, Enterobacter hormaechei, Eremothecium ashbyi and Lactococcus garvieae were assessed for the first time. The following TUs were not recommended for QPS status: A. caldiproteolyticus due to the lack of a body of knowledge in relation to its use in the food or feed chain, E. hormaechei, L. garvieae and K. ohmeri due to their pathogenic potential, E. ashbyi and C. stationis due to a lack of body of knowledge on their occurrence in the food and feed chain and to their pathogenic potential. B. paralicheniformis was recommended for the QPS status with the qualification 'absence of toxigenic activity' and 'absence of genetic information to synthesize bacitracin'.

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Production of Novel Polygalacturonase from Bacillus paralicheniformis CBS32 and Application to Depolymerization of Ramie Fiber

Cited by 21 publications

References 33 publications

Relationship of Polygalacturonase Enzyme Activity between Brown Rot Isolates and their Virulence on Potato Cultivars

Relationship of Polygalacturonase Enzyme Activity between Brown Rot Isolates and their Virulence on Potato Cultivars

Production of multi enzyme preparation by Bacillus subtilis using mosambi peel as substrate

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 14: suitability of taxonomic units notified to EFSA until March 2021

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