Production of NO and N(inf2)O by Pure Cultures of Nitrifying and Denitrifying Bacteria during Changes in Aeration

Kester, R.A.; Boer, Wietse de; Laanbroek, Hendrikus J.

doi:10.1128/aem.63.10.3872-3877.1997

Cited by 155 publications

(89 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the calculated net NO yield per NH z 4 oxidized might not reflect the gross NO yield by ammonia oxidizers in the sediment. The net NO yield observed in this study (5% at 46% air saturation or 125 mmol L 21 O 2 ) falls in between previously reported net NO yields in pure cultures (1.2% at 50% air saturation; Kester et al 1997) and in the oxygen minimum zone (OMZ) of the eastern tropical North Pacific (13% at O 2 , 100 mmol L 21 ; Ward and Zafiriou 1988).…”

Section: Discussionsupporting

confidence: 85%

See 1 more Smart Citation

Nitric oxide turnover in permeable river sediment

Schreiber

Stief

Kuypers

et al. 2014

Limnology & Oceanography

View full text Add to dashboard Cite

We measured nitric oxide (NO) microprofiles in relation to oxygen (O 2 ) and all major dissolved N-species (ammonium, nitrate, nitrite, and nitrous oxide [N 2 O]) in a permeable, freshwater sediment (River Weser, Germany). NO reaches peak concentrations of 0.13 mmol L 21 in the oxic zone and is consumed in the oxic-anoxic transition zone. Apparently, NO is produced by ammonia oxidizers under oxic conditions and consumed by denitrification under microoxic conditions. Experimental percolation of sediment cores with aerated surface water resulted in an initial rate of NO production that was 12 times higher than the net NO production rate in steady state. This initial NO production rate is in the same range as the net ammonia oxidation rate, indicating that NO is transiently the main product of ammonia oxidizers. Stable isotope labeling experiments with the 15 N-labeled chemical NO donor S-nitroso-N-acetylpenicillamine (SNAP) (1) confirmed denitrification as the main NO consumption pathway, with N 2 O as its major product, (2) showed that denitrification combines one free NO molecule with one NO molecule formed from nitrite to produce N 2 O, and (3) suggested that NO inhibits N 2 O reduction.

show abstract

Section: Discussionsupporting

confidence: 85%

“…Previous studies showed that nitrifier denitrification by AOB is active under microoxic conditions and is a source for NO formation (Poth and Focht 1985;Kester et al 1997). In line with this, the highest rates of NO production and NH z 4 consumption are expected to overlap, and NO production is expected to increase at low O 2 concentration.…”

Section: Discussionmentioning

confidence: 72%

Nitric oxide turnover in permeable river sediment

Schreiber

Stief

Kuypers

et al. 2014

Limnology & Oceanography

View full text Add to dashboard Cite

show abstract

“…Low oxygen conditions are known to induce clear increases in N 2 O production rates by Nitrosomonas sp. (Goreau et al, 1980;Kester et al, 1997;Dundee and Hopkins, 2001). The discussed data sets were all obtained using aerobic incubations.…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported oxygen sensitivity of N 2 O production in ammonia oxidizers (Goreau et al, 1980;Hynes and Knowles, 1984;Poth and Focht, 1985;Remde and Conrad, 1990;Kester et al, 1997;Dundee and Hopkins, 2001). For example, N 2 O production was shown to be greatly stimulated under anaerobic conditions (approximately 700 times greater that in aerobic conditions) in both N. europaea strain 28 and Nitrosospira (formerly Nitrosovibrio) strain K71 (Remde and Conrad, 1990).…”

Section: Discussionmentioning

confidence: 99%

“…As chemolithoautotrophs, they use ammonia as a sole source of energy and reducing power and obtain carbon for biosynthesis by fixation of CO 2 . Pure culture studies, mainly conducted with Nitrosomonas europaea strains (Ritchie and Nicholas, 1972;Hynes and Knowles, 1984;Poth and Focht, 1985;Anderson et al ., 1993;Kester et al ., 1997;Beaumont et al ., 2002), but also with Nitrosospira spp. (Goreau et al ., 1980;Remde and Conrad, 1990;Jiang and Bakken, 1999a;Dundee and Hopkins, 2001;Wrage et al ., 2004a), have shown that AOB also produce N 2 O.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nitrosospira spp. can produce nitrous oxide via a nitrifier denitrification pathway

Shaw

Nicol

Smith

et al. 2005

Environmental Microbiology

304

211

View full text Add to dashboard Cite

Nitrous oxide (N(2)O) emission from soils is a major contributor to the atmospheric loading of this potent greenhouse gas. It is thought that autotrophic ammonia oxidizing bacteria (AOB) are a significant source of soil-derived N(2)O and a denitrification pathway (i.e. reduction of NO(2) (-) to NO and N(2)O), so-called nitrifier denitrification, has been demonstrated as a N(2)O production mechanism in Nitrosomonas europaea. It is thought that Nitrosospira spp. are the dominant AOB in soil, but little information is available on their ability to produce N(2)O or on the existence of a nitrifier denitrification pathway in this lineage. This study aims to characterize N(2)O production and nitrifier denitrification in seven strains of AOB representative of clusters 0, 2 and 3 in the cultured Nitrosospira lineage. Nitrosomonas europaea ATCC 19718 and ATCC 25978 were analysed for comparison. The aerobically incubated test strains produced significant (P < 0.001) amounts of N(2)O and total N(2)O production rates ranged from 2.0 amol cell(-1) h(-1), in Nitrosospira tenuis strain NV12, to 58.0 amol cell(-1) h(-1), in N. europaea ATCC 19718. Nitrosomonas europaea ATCC 19718 was atypical in that it produced four times more N(2)O than the next highest producing strain. All AOB tested were able to carry out nitrifier denitrification under aerobic conditions, as determined by production of (15)N-N(2)O from applied (15)N-NO(2) (-). Up to 13.5% of the N(2)O produced was derived from the exogenously applied (15)N-NO(2) (-). The results suggest that nitrifier denitrification could be a universal trait in the betaproteobacterial AOB and its potential ecological significance is discussed.

show abstract

Nitrifying Bacteria

Spieck¹,

Bock²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Two groups of highly specialized organisms—the ammonia and the nitrite oxidizers—are called nitrifiers. The organisms are characterized by the capacity for lithotrophic growth in which energy is generated by the oxidation of ammonia to nitrite (ammonia oxidizers) or nitrite to nitrate (nitrite oxidizers). Nitrifiers are autotrophic bacteria, fixing CO2 as the main carbon source via the Calvin cycle. In addition to aerobic lithotrophic growth, the organisms possess a high metabolic diversity. The nitrogen of the biosphere exists in the oxidations states of NH 3 . Lithotrophic nitrification is considered to be the main process by which the transformation of more reduced nitrogen compounds to nitrate occurs. The nitrogen cycle is characterized by mobilization, immobilization, and transformation of the various nitrogen‐containing compounds. Nitrifying bacteria are of ecological importance because these organisms convert ammonia, which is often absorbed to soil particles, to nitrate, which is mobile in soil water. Although Nitrosomonas and Nitrobacter are the two most commonly isolated nitrifiers, they are not necessarily the most abundant ones in natural habitats. Their dominance in enrichment cultures may be explained by the fact that conditions in the commonly used enrichment culture methods promote the growth of these organisms.

show abstract

Production of NO and N(inf2)O by Pure Cultures of Nitrifying and Denitrifying Bacteria during Changes in Aeration

Cited by 155 publications

References 31 publications

Nitric oxide turnover in permeable river sediment

Nitric oxide turnover in permeable river sediment

Nitrosospira spp. can produce nitrous oxide via a nitrifier denitrification pathway

Nitrifying Bacteria

Contact Info

Product

Resources

About