Production of natural colorants by metabolically engineered microorganisms

Prabowo, Cindy Pricilia Surya; Eun, Hyunmin; Yang, Dongsoo; Hüccetoğulları, Damla; Raman, Jegadeesh; Kim, Soojin; Lee, Sang Yup

doi:10.1016/j.trechm.2022.04.009

Cited by 12 publications

(4 citation statements)

References 88 publications

(107 reference statements)

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“…To tackle this challenge, gene technology methods for producing natural dyes are being developed. For example, Saccharomyces cerevisiae and Escherichia coli could be used as hosts for dye-producing genes [ 11 , 12 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Toxicity Assessment of Cortinarius sanguineus Anthraquinone Aglycone Extract

Yli-Öyrä,

Herrala,

Kovakoski

et al. 2024

JoF

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Biocolourants could be a sustainable option for dyes that require fossil-based chemicals in their synthesis. We studied the in vitro toxicity of anthraquinone aglycone extract obtained from Cortinarius sanguineus fungus and compared it to the toxicity of its two main components, emodin and previously studied dermocybin. Cell viability, cytotoxicity, and oxidative stress responses in HepG2 liver and THP-1 immune cell lines were studied along with skin sensitisation. In addition, genotoxicity was studied with comet assay in HepG2 cells. Cellular viability was determined by MTT, propidium iodide, and lactate dehydrogenase assays, which showed that the highest doses of both the aglycone extract and emodin affected the viability. However, the effect did not occur in all of the used assays. Notably, after both exposures, a dose-dependent increase in oxidative stress factors was observed in both cell lines as measured by MitoSOX and dihydroethidium assays. C. sanguineus extract was not genotoxic in the comet assay. Importantly, both emodin and the extract activated the skin sensitisation pathway in the KeratinoSens assay, suggesting that they can induce allergy in humans. As emodin has shown cytotoxic and skin-sensitising effects, it is possible that the adverse effects caused by the extract are also mediated by it since it is the main component present in the fungus.

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Section: Introductionmentioning

confidence: 99%

In Vitro Toxicity Assessment of Cortinarius sanguineus Anthraquinone Aglycone Extract

Yli-Öyrä,

Herrala,

Kovakoski

et al. 2024

JoF

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“…Interestingly, colorant production with microbes could potentially solve many of these issues because microbes can be grown on a large scale on cheap substrates under controlled conditions in a sustainable manner. In this context, several biotechnological approaches have been recently developed that have enabled, for example, the production of carminic acid, a widely used red natural colorant found in scale insects, using genetically engineered microbes. − …”

Section: Introductionmentioning

confidence: 99%

Glycoside Phosphorylase Catalyzed Cellulose and β-1,3-Glucan Synthesis Using Chromophoric Glycosyl Acceptors

Pylkkänen,

Maaheimo,

Liljeström

et al. 2024

Biomacromolecules

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Glycoside phosphorylases are enzymes that are frequently used for polysaccharide synthesis. Some of these enzymes have broad substrate specificity, enabling the synthesis of reducing-end-functionalized glucan chains. Here, we explore the potential of glycoside phosphorylases in synthesizing chromophore-conjugated polysaccharides using commercially available chromophoric model compounds as glycosyl acceptors. Specifically, we report cellulose and β-1,3-glucan synthesis using 2-nitrophenyl β-d-glucopyranoside, 4-nitrophenyl β-d-glucopyranoside, and 2-methoxy-4-(2-nitrovinyl)phenyl β-d-glucopyranoside with Clostridium thermocellum cellodextrin phosphorylase and Thermosipho africanus β-1,3-glucan phosphorylase as catalysts. We demonstrate activity for both enzymes with all assayed chromophoric acceptors and report the crystallization-driven precipitation and detailed structural characterization of the synthesized polysaccharides, i.e., their molar mass distributions and various structural parameters, such as morphology, fibril diameter, lamellar thickness, and crystal form. Our results provide insights for the studies of chromophore-conjugated low molecular weight polysaccharides, glycoside phosphorylases, and the hierarchical assembly of crystalline cellulose and β-1,3-glucan.

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“…The application of such synthetic colors in different industries in general and in food and pharmaceutical industries in particular has raised several concerns and compulsory warnings by the FDA, World Health Organization and European Union regulators, especially for children's food, drugs and cosmetics [5]. Hence, due to various health problems associated with the overuse of synthetic colorants and the increasing customer and consumer awareness and demand for synthetic colorant-free products, scientific research has been oriented toward screening for natural colorants and improving their production and stability [6][7][8][9][10]. The majority of natural colorants also have nutritional and health benefits, especially as antioxidant agents [11,12].…”

Section: Introductionmentioning

confidence: 99%

Fungal Pigments: Their Diversity, Chemistry, Food and Non-Food Applications

Elkhateeb

Daba

2023

Applied Microbiology

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Colorants have many applications in food, cosmetics, pharmaceutics, textile, paints, plastics, paper, ink and photographic industries. Colorants are classified according to their solubility into dyes and pigments. Those of natural origin have many advantages over synthetic ones, as natural colorants usually do not induce allergies or other health problems. In addition, their consumption in the food and drug industries is fortified with nutritional and health benefits as the majority of them possess antioxidant activity or can be used to produce some vitamins. Plants, animals, insects and microorganisms are rich sources of colorants. However, microbial pigments are favored over other natural pigments due to their higher yield, stability, economical production. Therefore, we focus in this review on fungal pigments, the history of their use, their chemistry and their applications in food and non-food fields. Additionally, the ability of the fungal genus, Epicoccum, to produce pigments is discussed. Moreover, the challenges and future prospects concerning fungal pigment production are highlighted in detail.

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Production of natural colorants by metabolically engineered microorganisms

Cited by 12 publications

References 88 publications

In Vitro Toxicity Assessment of Cortinarius sanguineus Anthraquinone Aglycone Extract

In Vitro Toxicity Assessment of Cortinarius sanguineus Anthraquinone Aglycone Extract

Glycoside Phosphorylase Catalyzed Cellulose and β-1,3-Glucan Synthesis Using Chromophoric Glycosyl Acceptors

Fungal Pigments: Their Diversity, Chemistry, Food and Non-Food Applications

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