Production of nattokinase by batch and fed-batch culture of Bacillus subtilis

Cho, Young-Han; Song, Jae Yong; Kim, Kyung Mi; Kim, Mi Kyoung; Lee, In Young; Kim, Sang Bum; Kim, Hyeon Shup; Han, Nam Soo; Lee, Bong Hee; Kim, Beom Soo

doi:10.1016/j.nbt.2010.06.003

Cited by 53 publications

(50 citation statements)

References 21 publications

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“…Glucose concentration was determined using a Glucose E-kit (YD Diagnostics, Korea). Nattokinase activity was measured by modifying the fibrin degradation assay developed by JBSL (Japan Bio Science Laboratory Co., Ltd) [17]. First, 0.1 mL of diluted enzyme solution was added to 0.3 ml of 0.1 M Tris-HCl buffer (pH 7.8) containing 10 mM CaCl 2 and preincubated at 30°C water bath for 5 min.…”

Section: Discussionmentioning

confidence: 99%

“…We investigated the effects of type and concentration of complex medium on nattokinase production by B. subtilis [17]. With optimized medium composition, we monitored batch fermentation kinetics and found that nattokinase production was growth associated [17]. However, high cell density fermentations of B. subtilis have not been carried out to improve nattokinase activity.…”

Section: Introductionmentioning

confidence: 99%

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Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

Kwon

Kim

et al. 2011

Bioprocess Biosyst Eng

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Bacillus subtilis was cultivated to high cell density for nattokinase production by pH-stat fed-batch culture. A concentrated mixture solution of glucose and peptone was automatically added by acid-supplying pump when culture pH rose above high limit. Effect of the ratio of glucose to peptone in feeding solution was investigated on cell growth and nattokinase production by changing the ratio from 0.2 to 5 g glucose/g peptone. The highest cell concentration was 77 g/L when the ratio was 0.2 g glucose/g peptone. Cell concentration decreased with increasing the ratio of glucose to peptone in feeding solution, while the optimum condition existed for nattokinase production. The highest nattokinase activity was 14,500 unit/mL at a ratio of 0.33 g glucose/g peptone, which was 4.3 times higher than that in batch culture.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

Kwon

Kim

et al. 2011

Bioprocess Biosyst Eng

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“…and marine creatures (Mahajan et al 2012;Sumi et al 1992;Wang et al 2009c). There are comprehensive studies regarding the optimal fermentation conditions for NK production (Berenjian et al 2014a;Chen and Chao 2006;Chen et al 2007a;Cho et al 2010;Deepak et al 2008;Ku et al 2009;Kwon et al 2011;Liu et al 2005;Rasagnya and Vangalapati 2013;Unrean et al 2012;Wang et al 2009b;Mahajan et al 2010). Concentration adjustment and feeding strategy of essential nutrients are critical for enhancing NK production.…”

Section: Nattokinase Production and Formulationmentioning

confidence: 99%

Nattokinase: production and application

Dabbagh

Negahdaripour

Berenjian

et al. 2014

Appl Microbiol Biotechnol

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Nattokinase (NK, also known as subtilisin NAT) (EC 3.4.21.62) is one of the most considerable extracellular enzymes produced by Bacillus subtilis natto. The main interest about this enzyme is due to its direct fibrinolytic activity. Being stable enough in the gastrointestinal tract makes this enzyme a useful agent for the oral thrombolytic therapy. Thus, NK is regarded as a valuable dietary supplement or nutraceutical. Proven safety and ease of mass production are other advantages of this enzyme. In addition to these valuable advantages, there are other applications attributed to NK including treatment of hypertension, Alzheimer's disease, and vitreoretinal disorders. This review tends to bring a brief description about this valuable enzyme and summarizes the various biotechnological approaches used in its production, recovery, and purification. Some of the most important applications of NK, as well as its future prospects, are also discussed.

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“…subtilis RH44, and continuously fed by keeping glucose concentration within a range of 5-10 g L -1 which resulted in 2.5-fold higher riboflavin production than obtained in batch fermentation. Related with another enzyme, Cho et al [20] reported on influences of complex medium components in fed-batch nattokinase production by wild-type B. subtilis where glucose and peptone was continuously fed into the bioreactor by keeping the glucose concentration at 1 g L -1 . When the production medium containing peptone was used for fed-batch operation, 2.1-fold higher nattokinase activity was obtained compared to batch fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…When the production medium containing peptone was used for fed-batch operation, 2.1-fold higher nattokinase activity was obtained compared to batch fermentation. From the same research group, Kwon et al [21] developed a feeding strategy with a medium composition consisted of 1:3 glucose:peptone ratio, and achieved 4.3-fold increase in nattokinase activity compared to batch fermentation [20]. The evidence shows that there is no work in the literature on rhGH production using a fedbatch operation.…”

Section: Introductionmentioning

confidence: 99%

Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis

Şahin

Öztürk

Çalı́k

et al. 2015

Bioprocess Biosyst Eng

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Defined and semi-defined medium-based feeding strategies were developed to enhance recombinant human growth hormone (rhGH) production by Bacillus subtilis BGSC-1A178 (scoC (-)) strain carrying pMK4::pre(subC)::hGH. Defined medium-based feeding strategies were designed by exponential feeding of glucose and (NH4)2HPO4 at two pre-determined specific growth rates, µ 0 = 0.10 and 0.17 h(-1). Semi-defined medium-based feeding strategies were designed by exponential feeding of substrate solution consisting of glucose, (NH4)2HPO4, peptone, and trace salt solution (PTM1) at three pre-determined specific growth rates, µ 0 = 0.10, 0.17, and 0.25 h(-1). At all the strategies applied, transition cultivation time from batch to fed-batch operation was t T = 4 h. The highest rhGH concentration was obtained as C rhGH = 0.5 g L(-1) with semi-defined medium-based feeding strategy designed with µ 0 = 0.25 h(-1) using feed substrate stock solution containing 200 g L(-1) glucose, 117 g L(-1) (NH4)2HPO4, 100 g L(-1) peptone, and 5 mL L(-1) PTM1 at t = 22 h when the cell concentration reached to C X = 8.29 g L(-1). The overall product and cell yields on glucose were obtained as [Formula: see text] = 7.21 mg g(-1) and [Formula: see text] = 0.12 g g(-1), respectively. The results indicate the requirement of designing continuous feed stream in fed-batch production to enhance rhGH production by r-B. subtilis.

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Production of nattokinase by batch and fed-batch culture of Bacillus subtilis

Cited by 53 publications

References 21 publications

Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

Production of nattokinase by high cell density fed-batch culture of Bacillus subtilis

Nattokinase: production and application

Feeding strategy design for recombinant human growth hormone production by Bacillus subtilis

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