Production of mutants with high cold tolerance in spring canola (Brassica napus)

McClinchey, Scott L.; Kott, L. S.

doi:10.1007/s10681-007-9554-8

Cited by 45 publications

(32 citation statements)

References 49 publications

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“…O cultivo comercial de canola no mundo concentra-se em regiões temperadas, principalmente em latitudes superiores a 35º (McClinchey & Kott, 2008). A temperatura do ar é a variável ambiental mais importante na regulação do crescimento e desenvolvimento da canola (Thomas, 2003).…”

Section: Introductionunclassified

Aclimatação ao frio e dano por geada em canola

Dalmago

Cunha

Santi

et al. 2010

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a influência da aclimatação ao frio sobre o dano causado pela geada em diferentes estádios fenológicos de genótipos de canola. Foram realizados cinco experimentos em ambiente controlado, em 2006, 2007 e 2008. Os fatores avaliados foram: genótipos, aclimatação (com; sem), intensidades de geada, estádios de desenvolvimento de plantas, regimes de aclimatação e regimes de geada. As variáveis avaliadas foram: queima de folhas, massa de matéria seca, estatura de plantas, duração de subperíodo, componentes de rendimento e rendimento de grãos. A aclimatação ao frio, antes da geada, resultou em menor queima de folhas e maior massa de matéria seca, em comparação a plantas não aclimatadas. As geadas foram prejudiciais a partir de -6°C no início do ciclo de desenvolvimento, principalmente em plantas não aclimatadas, e a partir de -4ºC na floração, com redução do número de síliquas e do número de grãos por síliqua. A aclimatação após as geadas não contribuiu para a tolerância da canola a esse evento. Geadas consecutivas não acarretaram maior prejuízo à canola. A aclimatação de plantas de canola antes da geada reduz os danos, principalmente quando a geada ocorre no início do desenvolvimento das plantas.Termos para indexação: Brassica napus, estresse térmico, temperatura baixa, tolerância ao frio. Acclimatization to cold and frost-injury in canolaAbstract -The objective of this work was to evaluate the influence of cold acclimatization on frost damage at different phenological stages of canola genotypes. Five experiments were carried out under controlled conditions, in 2006, 2007, and 2008. The evaluated factors were: genotypes, acclimatization (with; without), frost gradient, plant developmental stages, acclimatization regimes and frost regimes. The evaluated variables were: leaf scorching symptoms, dry weight, plant height, length of subperiod, yield components and grain yield. The acclimatization before frost resulted in lesser leaf scorching symptoms and higher dry matter in comparison to plants not acclimated. Frosts were harmful from -6°C in early plant development stage, mainly in nonacclimated plants, and from -4ºC at flowering, reducing the number of pods and the number of grains per pod. Acclimatization after frost did not contribute to the tolerance of canola to frost. Consecutive frosts caused no greater impairment to canola. The canola acclimatization before frost reduces damage caused by it, mainly when the frost occur in the initial plant development stages.

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Section: Introductionunclassified

Aclimatação ao frio e dano por geada em canola

Dalmago

Cunha

Santi

et al. 2010

Pesq. agropec. bras.

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“…Differences in erucic acid levels and glucosinolate levels of B. carinata DH lines were reported after the microspores had been subjected to UV light or EMS (Barro et al 2001(Barro et al , 2002. Valuable agronomic characteristics have also been observed in DH plants regenerated from microspores exposed to UV (McClinchey and Kott 2008). In vitro selection was used in combination with UV mutagenesis to identify mutants with enhanced cold tolerance.…”

Section: Utilization Of Doubled Haploids In Mutagenesismentioning

confidence: 99%

“…The mutant lines were subjected to freezer tests and field testing to determine the level of cold tolerance. Several lines showed enhanced tolerance with one line advancing to yield trials (McClinchey and Kott 2008). The combination of EMS mutagenesis and in vitro selection has been used in a novel approach in order to produce lines resistant to Sclerotinia sclerotiorum (Liu et al 2005).…”

Section: Utilization Of Doubled Haploids In Mutagenesismentioning

confidence: 99%

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Ferrie

Möllers

2010

Plant Cell Tiss Organ Cult

101

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The availability of a highly efficient and reliable microspore culture protocol for many Brassica species makes this system useful for studying basic and applied research questions. Microspores and microspore-derived embryos are ideal targets for modification by mutagenesis and transformation. Regenerated doubled haploid plants are widely used in breeding programs and in genetic studies. Furthermore, the Brassica microspore culture system allows the identification of genomic regions and genes involved in the microspore embryogenic response, spontaneous diploidization and direct embryo to plant conversion. This review summarizes current achievements and discusses future perspectives.

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“…Many rapeseed varieties currently grown are hybrids of DH lines. Also, microspores and MDEs can be used for mutagenesis and in vitro selection (Albrecht et al 1995;Barro et al 2001;Ferrie et al 2008;McClinchey and Kott 2008) and it is possible to utilize microspores for genetic transformation (Guerche et al 1987;Fukuoka et al 1998;Nehlin et al 2000;Dormann et al 2001). The main advantage of androgenesis is, that traits can be fixed in one generation rather than after multiple selfpollinated generations (Dunwell 2010;Ferrie and Caswell 2011;Germanà 2011).…”

Section: Introductionmentioning

confidence: 99%

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the direct embryo to plant conversion rate of microspore-derived embryos

et al. 2016

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Microspore culture has become an important tool in many species, including Brassicas, for the production of entirely homozygous lines, so called double haploid (DH) lines. The primary products of microspore culture are embryo-like structures, called microspore-derived embryos (MDEs). A major problem in the development of DH lines is the often low efficiency of Direct Embryo to Plant Conversion (DEPC). During the development of DH populations, favourable alleles of genes affecting the DEPC rate will be under selection. This selection should lead to skewed segregations at markers linked to these genes. By comparing skewed marker segregations in four populations, a population of doubled haploid plantlets, a haploid and a doubled haploid MDE population, and a BC 1 population, 20 genomic regions were identified, which showed patterns of skewed segregations across the populations, indicative of the segregation of genetic factors controlling DEPC rates. Four regions and eight intervarietal substitution lines (ISLs) with donor segments overlapping these regions were selected for further studies. Three ISLs, ER654, ER661 and ER653 with DEPC rates of 49.1, 54.5 and 57.2 %, showed significantly reduced DEPC rates compared to the rate of the recurrent parent of 76.5 %. By comparing donor segments between the significant and the non-significant lines, eight genomic regions were identified that may contain genetic factors controlling the DEPC rate in rapeseed. These regions range in size from 0 (represented by just one marker) to 16.5 cM and cover together just 1.33 % of the genetic map used to characterize the donor segments in the ISLs.

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Production of mutants with high cold tolerance in spring canola (Brassica napus)

Cited by 45 publications

References 49 publications

Aclimatação ao frio e dano por geada em canola

Aclimatação ao frio e dano por geada em canola

Haploids and doubled haploids in Brassica spp. for genetic and genomic research

Identification and evaluation of intervarietal substitution lines of rapeseed (Brassica napus L.) with donor segments affecting the direct embryo to plant conversion rate of microspore-derived embryos

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