Production of moth sex pheromones for pest control by yeast fermentation

Holkenbrink, Carina; Ding, Bao‐Jian; Wang, Hong Lei; Dam, Marie Inger; Petkevicius, Karolis; Kildegaard, Kanchana Rueksomtawin; Wenning, Leonie; Sinkwitz, Christina; Lorántfy, Bettina; Koutsoumpeli, Eleni; França, Lucas; Pires, Marina J. Dias; Bernardi, Carmem; Urrutia, William; Mafra‐Neto, Agenor; Ferreira, Bruno Sommer; Raptopoulos, D.; Κωνσταντοπούλου, Μαρία; Löfstedt, Christer; Borodina, Irina

doi:10.1016/j.ymben.2020.10.001

Cited by 40 publications

(36 citation statements)

References 32 publications

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“…In response, recent studies have shown that pheromone components or precursors can be manufactured from engineered yeasts. The fermentation of the engineered yeast Y. lipolytica produced the sex pheromone of the Helicoverpa armigera worm, which was successfully efficient in field experiments [ 132 ].…”

Section: Yeasts As Bioinsecticide Bioherbicide and Biodegradermentioning

confidence: 99%

Culturable Yeasts as Biofertilizers and Biopesticides for a Sustainable Agriculture: A Comprehensive Review

Hernández-Fernández

Cordero-Bueso

Ruíz-Muñoz

et al. 2021

Plants

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The extensive use of synthetic fertilizers and pesticides has negative consequences in terms of soil microbial biodiversity and environmental contamination. Faced with this growing concern, a proposed alternative agricultural method is the use of microorganisms as biofertilizers. Many works have been focused on bacteria, but the limited literature on yeasts and their potential ability to safely promote plant growth is gaining particular attention in recent years. Thus, the objective of this review is to highlight the application of yeasts as biological agents in different sectors of sustainable agricultural practices through direct or indirect mechanisms of action. Direct mechanisms include the ability of yeasts to provide soluble nutrients to plants, produce organic acids and phytohormones (indole-3-acetic acid). Indirect mechanisms involve the ability for yeasts to act as biocontrol agents through their high antifungal activity and lower insecticidal and herbicidal activity, and as soil bioremediating agents. They also act as protective agents against extreme environmental factors by activating defense mechanisms. It is evident that all the aspects that yeasts offer could be useful in the creation of quality biofertilizers and biopesticides. Hence, extensive research on yeasts could be promising and potentially provide an environmentally friendly solution to the increased crop production that will be required with a growing population.

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Section: Yeasts As Bioinsecticide Bioherbicide and Biodegradermentioning

confidence: 99%

Culturable Yeasts as Biofertilizers and Biopesticides for a Sustainable Agriculture: A Comprehensive Review

Hernández-Fernández

Cordero-Bueso

Ruíz-Muñoz

et al. 2021

Plants

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“…This research was initiated as a Synthetic Biology project in the frame of the iGEM competition, where undergraduate students proposed the use of genetically engineered plants as dispensers of insect sex pheromones. The manufacturing of semiochemicals and their precursors employing biological factories such as microbial bioreactors (Hagström et al, 2013;Holkenbrink et al, 2020) or plant biofactories (Ding et al, 2014;Ortiz et al, 2020;Xia et al, 2020) has become an intensively pursued objective, fuelled by the expected gains in sustainability. Beyond the general biofactory concept, our envisioned long-term approach consists in the design of plants that function as autonomous bio-dispensers of semiochemicals.…”

Section: Discussionmentioning

confidence: 99%

Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

Mateos-Fernández

Moreno-Giménez

Gianoglio

et al. 2021

Preprint

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Plant-based bio-production of insect sex pheromones has been proposed as an innovative strategy to increase the sustainability of pest control in agriculture. Here we describe the engineering of transgenic plants producing (Z)-11-hexadecen-1-ol (Z11-16OH) and (Z)-11-hexadecenyl acetate (Z11-16OAc), two main volatile components in many Lepidoptera sex pheromone blends. We assembled multiple multigene DNA constructs encoding the pheromone biosynthetic pathway and stably transformed them in Nicotiana benthamiana plants. The constructs comprised the Amyelois transitella AtrΔ11 desaturase gene, the Helicoverpa armigera farnesyl reductase HarFAR gene, and the Euonymus alatus diacylglycerol acetyltransferase EaDAct gene in different configurations. All the pheromone-producing plants showed dwarf phenotypes, whose severity correlated with pheromone levels. All but one of the recovered lines produced high levels of Z11-16OH but very low levels of Z11-16OAc, probably as a result of recurrent truncations at the level of the EaDAct gene. Only one plant line (SxPv1.2) was recovered harbouring an intact pheromone pathway and producing moderate levels of Z11-16OAc (11.8 μg g-1 FW), next to high levels of Z11-16OH (111.4 μg g-1). Z11-16OAc production was accompanied in SxPv1.2 by a partial recovery of the dwarf phenotype. SxPv1.2 was used to estimate the rates of volatile pheromone release, which resulted in 8.48 ng g-1 FW per day for Z11-16OH and 9.44 ng g-1 FW per day for Z11-16OAc. Our results suggest that pheromone release acts as a limiting factor in pheromone bio-dispenser strategies and establish a roadmap for biotechnological improvements.

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“…Fatty Alcohol Production by Y. lipolytica Y. lipolytica has emerged as a model oleaginous yeast and therefore has benefitted from the development of numerous genetic tools. In 2016, four separate reports of fatty alcohol production in Y. lipolytica were published (Rutter and Rao, 2016;Wang G. et al, 2016;Wang W. et al, 2016;Xu et al, 2016) and since 2016 four additional studies have investigated engineering Y. lipolytica for fatty alcohol biosynthesis (Dahlin et al, 2019;Zhang et al, 2019;Cordova et al, 2020;Holkenbrink et al, 2020).…”

Section: Engineering Fatty Alcohol Production In Oleaginous Yeastsmentioning

confidence: 99%

“…A strain deficient in aldehyde dehydrogenases hfd1, hfd4, fatty alcohol oxidase fao1 and peroxisomal biogenesis factor pex10 has been engineered to produce cis-11-hexadecanol, an insect pheromone, at a titer of 2.75 g L −1 through expression of acyl-CoA desaturase from Amyelois tansitella and FAR enzymes from Heliothis subflexia and Helicoverpa armigra. These authors were also able to produce cis-9-tetradecanol by introducing a mutation into fatty acyl-CoA synthetase, Fas2 I1220F and expression of D. melanogaster acyl-CoA desaturase and H. armigra FAR (Holkenbrink et al, 2020). Y. lipolytica has been engineered to produce the medium chain fatty alcohol 1-decanol by expression of acyl-ACP-thioesterases from A. tetadius, C. hookeriana, C. palustris, C. perfringens, and U. californica with specificity to produce 8-10 carbon chain fatty acids and AtFAR6 (Rutter and Rao, 2016).…”

Section: Engineering Fatty Alcohol Production In Oleaginous Yeastsmentioning

confidence: 99%

Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations

Krishnan

McNeil

Stuart

2020

Front. Bioeng. Biotechnol.

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Concerns about climate change and environmental destruction have led to interest in technologies that can replace fossil fuels and petrochemicals with compounds derived from sustainable sources that have lower environmental impact. Fatty alcohols produced by chemical synthesis from ethylene or by chemical conversion of plant oils have a large range of industrial applications. These chemicals can be synthesized through biological routes but their free forms are produced in trace amounts naturally. This review focuses on how genetic engineering of endogenous fatty acid metabolism and heterologous expression of fatty alcohol producing enzymes have come together resulting in the current state of the field for production of fatty alcohols by microbial cell factories. We provide an overview of endogenous fatty acid synthesis, enzymatic methods of conversion to fatty alcohols and review the research to date on microbial fatty alcohol production. The primary focus is on work performed in the model microorganisms, Escherichia coli and Saccharomyces cerevisiae but advances made with cyanobacteria and oleaginous yeasts are also considered. The limitations to production of fatty alcohols by microbial cell factories are detailed along with consideration to potential research directions that may aid in achieving viable commercial scale production of fatty alcohols from renewable feedstock.

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Production of moth sex pheromones for pest control by yeast fermentation

Cited by 40 publications

References 32 publications

Culturable Yeasts as Biofertilizers and Biopesticides for a Sustainable Agriculture: A Comprehensive Review

Culturable Yeasts as Biofertilizers and Biopesticides for a Sustainable Agriculture: A Comprehensive Review

Production of Volatile Moth Sex Pheromones in Transgenic Nicotiana benthamiana Plants

Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations

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