Production of monoclonal shark-derived immunoglobulin new antigen receptor antibodies using Chinese hamster ovary cell expression system

Enatsu, Hajime; Okamoto, Nako; Nomura, Yoshiki; Onitsuka, Masayoshi; Yamano-Adachi, Noriko; Koga, Yuichi; Ômasa, Takeshi

doi:10.1016/j.jbiosc.2021.04.015

Cited by 3 publications

(1 citation statement)

References 30 publications

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“…In eukaryotic cell culture systems, the Chinese Hamster Ovary (CHO) cells are mainly used for the mass-production of the high-quality therapeutic recombinant proteins and monoclonal antibodies (mAbs; Sharker and Rahman, 2021 ), such as the spike ectodomain of SARS-CoV-2 ( Djaileb et al, 2021 ), a MERS-coronavirus vaccine antigen ( Nyon et al, 2018 ), the mAbs of structurally unstable G protein-coupled receptor CC chemokine receptor 9 ( Nanamiya et al, 2021 ), the immunoglobulin new antigen receptor of cartilaginous fishes ( Enatsu et al, 2021 ), and functional chimeric sIgA ( Berdoz et al, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets

Xiao

Zhang²,

Wang³

et al. 2022

Front. Microbiol.

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Immunoglobulin A (IgA) of sows is critically important for assessing piglets’ protective capacity against porcine epidemic diarrhea virus (PEDV). Here, we report a therapeutic chimeric anti-PEDV IgG/IgA expressed by Chinese hamster ovary (CHO) cells for oral treatment of PED. The chimeric anti-PEDV IgG/IgA was produced by the CHO cell lines, in which the heavy chain was constructed by combining the VH, Cγ1 and hinge regions of PEDV IgG mAb 8A3, and the Cα2 and Cα3 domains of a Mus musculus immunoglobulin alpha chain. The chimeric anti-PEDV IgG/IgA could neutralize the strains of CV777 (G1), P014 (G2) and HN1303 (G2) in vitro effectively, showing broad-spectrum neutralization activity. The in vivo challenge experiments demonstrated that chimeric anti-PEDV IgG/IgA (9C4) produced in the CHO cell supernatant could alleviate clinical diarrhea symptoms of the PEDV infection in piglets. In general, our study showed that chimeric anti-PEDV IgG/IgA produced from CHO cell line supernatants effectively alleviates PEDV infection in piglets, which also gives the foundation for the construction of fully functional secretory IgA by the J chain introduction to maximize the antibody therapeutic effect.

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Section: Introductionmentioning

confidence: 99%

A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets

Xiao

Zhang²,

Wang³

et al. 2022

Front. Microbiol.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Expansion of Chinese hamster ovary cells via a loose cluster-assisted suspension culture using cell-sized gelatin microcarriers

Hasebe

Yamada

Utoh

et al. 2023

Journal of Bioscience and Bioengineering

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Production of monoclonal shark-derived immunoglobulin new antigen receptor antibodies using Chinese hamster ovary cell expression system

Cited by 3 publications

References 30 publications

A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets

A therapeutic chimeric IgG/IgA expressed by CHO cells for oral treatment of PED in piglets

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Expansion of Chinese hamster ovary cells via a loose cluster-assisted suspension culture using cell-sized gelatin microcarriers

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