Production of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting

Faerman, Alexander; Chervonskii, A. V.; Chipysheva, T. A.; Gel'shtein, V. I.; Kolyada, Alexey

doi:10.1007/bf00841850

Cited by 3 publications

(1 citation statement)

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“…FITC-conjugated goat anti-mouse IgG (1:lOO) was obtained from Sigma, St. Louis, MO, TRITC-conjugated goat anti-mouse IgG (1:80) from Cappel, West Chester, PA, and FITC-conjugated swine anti-rabbit IgG (1:20) from Dakopatts (Copenhagen, Denmark). Monoclonal antiperoxidase antibody (Faerman et al, 1987) for PAP reaction was kindly given by Dr. A.V. Chervonsky (our Institute).…”

Section: Methodsmentioning

confidence: 99%

Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer

Guelstein¹,

Tchypysheva²,

Ермилова³

et al. 1988

Intl Journal of Cancer

125

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The distribution of keratins 8 and 17 and of vimentin in 28 normal human mammary tissue samples, 16 benign tumors, 26 fibrocytic diseases and 52 malignant breast tumors have been studied using monoclonal antibodies HI, E3 and NT30, respectively. Three cell populations in normal mammary epithelium have been identified: luminal epithelium containing keratin 8, myoepithelium of the lobular structures positive for vimentin, and myoepithelium of extralobular ducts positive for keratin 17. In different kinds of benign tumor and dysplastic proliferation a mosaic of cells with all normal phenotypes has been observed. The majority of cells co-expressed keratins 8 and 17 or vimentin. In the overwhelming majority of carcinomas, cells did not contain myoepithelial markers (keratin 17 and vimentin) but expressed only keratin 8 specific to normal luminal epithelium.

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Section: Methodsmentioning

confidence: 99%

Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer

Guelstein¹,

Tchypysheva²,

Ермилова³

et al. 1988

Intl Journal of Cancer

125

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Immunohistochemical study of angiotensin-converting enzyme in human tissues using monoclonal antibodies

et al. 1987

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The localization of angiotensin-converting enzyme (ACE) in human tissues has been studied by the PAP-method with the use of monoclonal antibody 9 B9 against human lung ACE. The enzyme was detected on the surface of endothelial cells in lung, myocardium, liver, intestine and testis as well as in the epithelial cells of the kidney proximal tubules and intestine. The monoclonal antibody 9 B9 did not react with ACE in the epithelial cells of the testis seminiferous tubules. These data suggest that the antibody 9 B9 recognizes epitope which is shared by the ACE molecule of endothelial cells and renal and intestinal epithelial cells but is not present in testicular ACE, or is not accessible there to the antibody.

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Human corneal endothelial cells: Isolation, characterization and long-term cultivation

Pistsov

Sadovnikova

Danilov

1988

Experimental Eye Research

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Production of monoclonal antibodies to horseradish peroxidase and their use in immunohistochemistry and immunoblotting

Cited by 3 publications

References 0 publications

Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer

Monoclonal antibody mapping of keratins 8 and 17 and of vimentin in normal human mammary gland, benign tumors, dysplasias and breast cancer

Immunohistochemical study of angiotensin-converting enzyme in human tissues using monoclonal antibodies

Human corneal endothelial cells: Isolation, characterization and long-term cultivation

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