Production of monoclonal antibodies for the detection of potato virus Y

D, Rose; Hubbard, A. L.

doi:10.1111/j.1744-7348.1986.tb05323.x

Cited by 30 publications

(14 citation statements)

References 15 publications

(13 reference statements)

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“…To determine the reactivity of antibodies to different PVY isolates, comparisons were made with three additional PVY MAbs: PVYN 295.5 (Rose & Hubbard 1986), 1F5 (Ellis et al 1996), and PVY° Mab 2 (McDonald & Kristjansson 1993).…”

Section: Methodsmentioning

confidence: 99%

Coat protein and 5' nontranslated region of a variant of potato virus Y

McDonald

Wong

Henning

et al. 1997

Canadian Journal of Plant Pathology

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Section: Methodsmentioning

confidence: 99%

Coat protein and 5' nontranslated region of a variant of potato virus Y

McDonald

Wong

Henning

et al. 1997

Canadian Journal of Plant Pathology

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“…For example, various equipment exists to automate the different coating and washing stages, and reading and recording of test results. It is currently the method of choice for testing microplants and growing crop inspection leaf samples for the presence of potato virus (Browning, 1995;Hadidi et al, 1993;Rose & Hubbard, 1986;Schroeder & Weidemann, 1990).…”

Section: Serological Diagnosticsmentioning

confidence: 99%

Plant pathogen diagnostics: present status and future developments

O'Donnell

1999

Potato Res

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“…In the application of MoAbs in enzyme-linked immunosorbent assay (ELISA) for viral detection in infected plants, indirect double antibody sandwich ELISA (IDAS-ELISA) using PoAbs as trapping antibody has been mostly employed5, 7,11,13,14) . The disadvantage of this system is that the PoAbs must be prepared in addition to the MoAbs .…”

Section: Introductionmentioning

confidence: 99%

Relationship between hybridoma screening procedures and the characteristics of monoclonal antibodies for use in direct double antibody sandwich ELISA for the detection of plant viruses.

Ohshima

Harjosudarmo

Ishikawa

et al. 1990

Jpn. J. Phytopathol.

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More than two hundred and fifty-five monoclonal antibody (MoAb)-secreting hybridomas against 3 plant luteoviruses, 2 plant reoviruses and a potyvirus were produced.The hybridomas for potato leafroll virus, beet western yellows virus, tobacco necrotic dwarf virus, rice dwarf virus, rice ragged stunt virus and potato virus Y-ordinary strain, were screened by four different procedures of enzyme-linked immunosorbent assay (ELISA); procedure 1, antigen adsorption indirect ELISA (AAI-ELISA) in which purified virus in phosphate buffered saline (PBS) at pH 7.4 was adsorbed onto the microplate wells, procedure 2, AAI-ELISA in which purified virus in sodium carbonate-bicarbonate buffer at pH 9.6 was adsorbed onto the microplate wells, procedure 3, indirect double antibody sandwich ELISA (IDAS-ELISA) in which polyclonal antibody was used as trapping antibody and purified virus preparations diluted in PBS-T (containing Tween-20) as antigens were used, procedure 4, IDAS-ELISA in which polyclonal antibody was used for trapping antibody and crude saps of virus infected plants extracted in PBS-T as antigens were used. Based on the MoAb reactivities against homologous viruses in four different ELISA procedures, MoAb-secreting hybridomas were divided into ten groups. Using purified MoAbs from ascitic fluids, direct double antibody sandwich ELISA (DAS-ELISA) was examined for the detection of virus antigens in infected plants. All the MoAbs reacted in DAS-ELISA belonged to group 1 in which the MoAbs were reactive in each four of the screening procedures 1-4, or group 2 in which MoAbs were reactive in three of screening procedures 1, 3 and 4. These results indicate that MoAbs being reactive in DAS-ELISA can be readily selected in hybridomas in group 1 or 2.

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Production of monoclonal antibodies for the detection of potato virus Y

Cited by 30 publications

References 15 publications

Coat protein and 5' nontranslated region of a variant of potato virus Y

Coat protein and 5' nontranslated region of a variant of potato virus Y

Plant pathogen diagnostics: present status and future developments

Relationship between hybridoma screening procedures and the characteristics of monoclonal antibodies for use in direct double antibody sandwich ELISA for the detection of plant viruses.

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