Production of mono-olein by immobilized Staphylococcus simulans lipase in a solvent-free system: Optimization by response surface methodology

Ghamgui, Hanen; Miled, Nabil; Rebaï, Ahmed; Karra-Châabouni, Maha; Gargouri, Youssef

doi:10.1016/j.enzmictec.2005.12.014

Cited by 58 publications

(29 citation statements)

References 20 publications

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“…The ''Lack of Fit F-value'' is not significant. The high R 2 values (>0.9) for all wood samples demonstrate that there is good agreement between the experimental results and the theoretical values predicted by the model [11].…”

Section: Analysis Of Variance (Anova)supporting

confidence: 69%

“…It is also a collection of mathematical and statistical procedures that are useful for the modeling and analysis of problems in which the response of demand is affected by several variables and the objective is to optimize the response [10]. Through this design, linear, quadratic and cross-product effects were analyzed to reach at a predictive equation [11] for the yield of CO 2 where three variables were employed in setting up the equation. In this study, central composite design is used to predict the optimum conditions for maximum CO 2 using the fitted second-order polynomial regression model or quadratic model.…”

Section: Optimizationmentioning

confidence: 99%

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CO2 production from degraded woods via a novel integrated pyrolysis-combustion process

Othman

Kamarudin

Yunus

et al. 2009

Journal of Analytical and Applied Pyrolysis

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Section: Analysis Of Variance (Anova)supporting

confidence: 69%

Section: Optimizationmentioning

confidence: 99%

CO2 production from degraded woods via a novel integrated pyrolysis-combustion process

Othman

Kamarudin

Yunus

et al. 2009

Journal of Analytical and Applied Pyrolysis

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“…Kristensen et al (2005aKristensen et al ( , 2005b reported that the high amount of enzyme in viscous oil system could cause improper mixing of reaction and thereby promoting limitations to mass transfer. This phenomenon may also relate to the possible protein aggregations that prevented the exposure of enzyme active site to the substrate (Ghamgui et al 2006). Figure 3c shows that the MLCT (y) decreased to center point and continuously decreased to the maximum ratio of Robles Medina et al (1999), andErgan et al (1990) reported that the TAG yield decreased as the ratio of FA/glycerol increased.…”

Section: Model Fittingmentioning

confidence: 84%

Modeling and Optimization of Lipozyme RM IM-Catalyzed Esterification of Medium- and Long-Chain Triacyglycerols (MLCT) Using Response Surface Methodology

Arifin

Soo-Peng

Long

et al. 2010

Food Bioprocess Technol

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Optimization conditions of Lipozyme RM IM lipase esterification of capric and stearic acids with glycerol for the production of medium-and long-chain triacyglycerols (MLCT) fat suitable for food applications such as margarine and shortening were investigated. Response surface methodology (RSM) was applied to model and optimize the reaction conditions, namely, the reaction time (8-24 h), enzyme load (5-15 wt.%), and fatty acids/ glycerol ratio (3:1-4:1) and represented by Ti, En, and Sb, respectively. Best-fitting models were successfully established for both MLCT yield (R 2 =0.9507) and residual FFA (R 2 =0.9315) established by multiple regressions with backward elimination. Optimal reaction conditions were 13.6-14.0 h for reaction time, 7.9-8.0 wt.% for enzyme load, and 3:1 for fatty acids/glycerol molar ratio. Chi-square test showed that there were no significant (P>0.05) differences between the observed and predicted values of both models. Refined MLCT fat blend had sufficient solid fat at room temperature and made it suitable to use as a hard stock in shortening and margarine production.

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“…Monoacylglycerols (MAGs) are nonionic surfactants and emulsifiers with hydrophilic and hydrophobic parts in molecules [1]. They are widely used in food industry with applications in dairy products, margarines, bakery products, and sauces.…”

Section: Introductionmentioning

confidence: 99%

Optimization of mono and diacylglycerols production from enzymatic glycerolysis in solvent-free systems

Valério

Rovani

Treichel

et al. 2009

Bioprocess Biosyst Eng

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This work reports solvent-free enzymatic glycerolysis of olive oil with an immobilized lipase (Novozym 435) using Tween 40, Tween 65, Tween 80, Tween 85, Triton X-100, and soy lecithin as surfactants. The first step was the screening of two potential surfactants for Monoacylglycerol (MAG) and Diacylglycerol (DAG) production with a pre-established operating condition and 2 h of reaction time. Afterwards, a sequential experimental design strategy was carried out in order to optimize MAG and DAG production using Tween 65 and Triton X-100 as surfactants. The operating conditions that optimized MAG and DAG yields were 70 degrees C, stirring rate of 600 rpm, glycerol:olive oil molar ratio of 6:1, 16 wt% of surfactant Tween 65 and 9.0 wt% of Novozym 435, leading to a content of 26 and 17 wt% of MAG and DAG, respectively.

show abstract

Production of mono-olein by immobilized Staphylococcus simulans lipase in a solvent-free system: Optimization by response surface methodology

Cited by 58 publications

References 20 publications

CO2 production from degraded woods via a novel integrated pyrolysis-combustion process

CO2 production from degraded woods via a novel integrated pyrolysis-combustion process

Modeling and Optimization of Lipozyme RM IM-Catalyzed Esterification of Medium- and Long-Chain Triacyglycerols (MLCT) Using Response Surface Methodology

Optimization of mono and diacylglycerols production from enzymatic glycerolysis in solvent-free systems

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