Production of Microbial Cellulose by a Bacterium Isolated from Fruit

Abstract

This study presents the production of bacterial cellulose (BC) by a bacterium isolated from a rotten fruit and its process optimization. Here, isolation and screening of potent cellulose producers were carried out from different natural sources, viz., soil, rotten fruits, and vegetables and vinegar. A total of 200 bacterial isolates were obtained, which were screened for cellulose production using Hestrin-Schramm medium. A novel and potent cellulose-producing bacterium was newly isolated from a rotten fruit and identified as Gluconacetobacter sp. F6 through 16S ribosomal DNA sequencing and morphological, cultural, and biochemical characteristics. After optimization of culture conditions, including pH, temperature, agitation, carbon/nitrogen sources, and inducers, the BC production was greatly increased from 0.52 to 4.5 g/l (8.65-fold increase). The optimal culture medium contained 1% (w/v) glucose, 1.5% (w/v) yeast extract, 0.5% (w/v) peptone, 0.27% (w/v) disodium hydrogen phosphate, 0.115% (w/v) citric acid, and 0.4% (w/v) ethanol. BC produced was analyzed for the presence of cellulose fibrils by epiflourescent microscopy using Calcofluor white stain and scanning electron microscopy and confirmed by NMR. There are very scanty reports about the optimization of BC production by bacteria isolated from rotten fruits.

“Deceived” Concentrated Immobilized Cells as Biocatalyst for Intensive Bacterial Cellulose Production from Various Sources

“…For instance, it was revealed that the increase in cell inoculum concentration over 5% (v/v) lowers the BC yield [42]. A similar conclusion done by other researches experimentally revealed how the amount of cell inoculum influences the BC production [43]. It appeared that when the inoculum concentration was decreased from 6-4% (v/v), the BC accumulation was increased, and further increasing or decreasing the inoculum from 4%, a decline in BC production was observed.…”

Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits

“…; Jahan et al . ), whereas some researches indicated pH 4–6 as the optimum pH range (Fontana et al . ; Masaoka et al .…”

“…Besides that, most of the biocellulose‐producing bacterial strains were obtained from fruits such as apple, orange, tomato, grape and wild lemon (Jahan et al . ; Tanskul et al . ).…”

Bacterial Cellulose Fibers

“…A new bacterium (Gluconacetobacter sp. F6) was isolated from rotten fruit and the conditions required to obtain optimum cellulose from the fruit waste were studied [12Jah]. In addition to rotten fruit, soil, vegetables, and vinegar were also studied as potential sources for bacterium.…”

“…In addition to rotten fruit, soil, vegetables, and vinegar were also studied as potential sources for bacterium. A cellulose yield of 4.5 g/l was obtained under the optimum conditions of pH 6, temperature of 30 C, and using glucose as the carbon source [12Jah]. A thick leathery pellicle formed during production of grape wine was studied for its structure and properties and identified as bacterial cellulose from the Gluconacetobacter sp.…”