Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells

Earle, Wilton R.; Schilling, Edward L.; Stark, Thomas; Straus, Nancy P.; Brown, M.; Shelton, Emma

doi:10.1093/jnci/4.2.165

Cited by 164 publications

(8 citation statements)

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“…On the other hand, L-929 cells demonstrate a viability of 89.26 ± 7.42%, revealing to be the most resistant cells, in terms of viability, in all the included studies. This result is predictable, taking into consideration that L-929 cells derive from an immortalized cell line (Earle et al, 1943). Finally, HUVECs and C2C12 show less viability than other cell lines with 78.42 ± 19.3 and 80.71 ± 19.6%, respectively.…”

Section: Cellular Testsmentioning

confidence: 63%

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Sánchez¹,

Gómez-Blanco²,

Nieto

et al. 2020

Front. Bioeng. Biotechnol.

119

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Nowadays, bioprinting is rapidly evolving and hydrogels are a key component for its success. In this sense, synthesis of hydrogels, as well as bioprinting process, and cross-linking of bioinks represent different challenges for the scientific community. A set of unified criteria and a common framework are missing, so multidisciplinary research teams might not efficiently share the advances and limitations of bioprinting. Although multiple combinations of materials and proportions have been used for several applications, it is still unclear the relationship between good printability of hydrogels and better medical/clinical behavior of bioprinted structures. For this reason, a PRISMA methodology was conducted in this review. Thus, 1,774 papers were retrieved from PUBMED, WOS, and SCOPUS databases. After selection, 118 papers were analyzed to extract information about materials, hydrogel synthesis, bioprinting process, and tests performed on bioprinted structures. The aim of this systematic review is to analyze materials used and their influence on the bioprinting parameters that ultimately generate tridimensional structures. Furthermore, a comparison of mechanical and cellular behavior of those bioprinted structures is presented. Finally, some conclusions and recommendations are exposed to improve reproducibility and facilitate a fair comparison of results.

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Section: Cellular Testsmentioning

confidence: 63%

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Sánchez¹,

Gómez-Blanco²,

Nieto

et al. 2020

Front. Bioeng. Biotechnol.

119

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“…The mouse areolar adipose tissue cell line L929S was obtained from European Collection of Authenticated Cell Cultures (ECACC Catalogue No. : 86032004, supplied by Sigma-Aldrich) . Cells were resuscitated and cultured with Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 1 g/L glucose, 2 mM glutamine, 10% fetal bovine serum (FBS), and 1% penicillin–streptomycin (PenStrep) in a humidified incubator at 37 °C with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

“…: 86032004, supplied by Sigma-Aldrich). 31 Cells were resuscitated and cultured with Dulbecco's Modified Eagle Medium (DMEM) supplemented with 1 g/L glucose, 2 mM glutamine, 10% fetal bovine serum (FBS), and 1% penicillin− streptomycin (PenStrep) in a humidified incubator at 37 °C with 5% CO 2 . Subculture of cells was performed as suggested by ECACC.…”

mentioning

confidence: 99%

Generation of ¹³C-Labeled Inositol and Inositol Phosphates by Stable Isotope Labeling Cell Culture for Quantitative Metabolomics

Lämmerhofer

2022

Anal. Chem.

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Inositol and inositol phosphates (IPx) are central metabolites. Their accurate quantitative analysis in complex biological samples is challenging due to lengthy sample preparation procedures, sample losses by strong adsorption to surfaces, and unpredictable matrix effects. Currently, U 13 C-inositol and U 13 C-IPx are not available from commercial sources. In this study, we developed a method that is capable of generating U 13 C-inositol and U 13 C-IPx. An inositol-independent cell line L929S was cultured in inositol-free medium supplemented with U 13 C-glucose. Inositol contamination in FBS was observed as the critical parameter for labeling efficiency (LE). A balance between cell growth and LE was achieved by adopting a two-step labeling strategy. In the first step, a LE of 90% could be obtained by normal cell growth in the long-term. Cells were then cultured in a second step in ultralabeling medium for improved LE for a short duration before harvesting. The generated U 13 Canalogs were of high isotopic purity (>99%). Utilized as internal standards spiked before sample preparation in biological applications, U 13 Canalogs can effectively compensate sample loss during sample preparation as well as the matrix effect during electrospray ionization. An exemplary pharmacological study was conducted with phospholipase C inhibitor and activator to document the great utility of the prepared stable isotope-labeled internal standards in elucidating the PLC-dependent IP code. U 13 CIPx are used as internal standards to generate quantitative profiles of IPx in HeLa cell samples after treatment with PLC inhibitor and activator. This established method generating U 13 Canalogs is cost-effective, robust, and reproducible, which can facilitate quantitative studies of inositol and IPx in biological scenarios.

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“…However, somatic cells derived from animals typically died after a definite number of divisions, imposing fresh cell preparation for each experiment. A turning point for cell culture advancement was 1943, when Wilton Robinson Earle generated the first continuous cell line, the "L" cell line, from subcutaneous mouse tissue [7]. A few years later, in 1951, George Otto Gey established the first human immortal tumoral cell line, the HeLa cells, derived from cancerous tissue of the cervix of Henrietta Lacks, a 30-year-old mother of five, who died of cervical cancer on 4 October 1951 [8].…”

Section: Rise and Fall Of 2d Cell Culturesmentioning

confidence: 99%

Multicellular 3D Models to Study Tumour-Stroma Interactions

Colombo

Cattaneo

2021

IJMS

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Two-dimensional (2D) cell cultures have been the standard for many different applications, ranging from basic research to stem cell and cancer research to regenerative medicine, for most of the past century. Hence, almost all of our knowledge about fundamental biological processes has been provided by primary and established cell lines cultured in 2D monolayer. However, cells in tissues and organs do not exist as single entities, and life in multicellular organisms relies on the coordination of several cellular activities, which depend on cell–cell communication across different cell types and tissues. In addition, cells are embedded within a complex non-cellular structure known as the extracellular matrix (ECM), which anchors them in a three-dimensional (3D) formation. Likewise, tumour cells interact with their surrounding matrix and tissue, and the physical and biochemical properties of this microenvironment regulate cancer differentiation, proliferation, invasion, and metastasis. 2D models are unable to mimic the complex and dynamic interactions of the tumour microenvironment (TME) and ignore spatial cell–ECM and cell–cell interactions. Thus, multicellular 3D models are excellent tools to recapitulate in vitro the spatial dimension, cellular heterogeneity, and molecular networks of the TME. This review summarizes the biological significance of the cell–ECM and cell–cell interactions in the onset and progression of tumours and focuses on the requirement for these interactions to build up representative in vitro models for the study of the pathophysiology of cancer and for the design of more clinically relevant treatments.

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Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells

Cited by 164 publications

References 0 publications

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Generation of ¹³C-Labeled Inositol and Inositol Phosphates by Stable Isotope Labeling Cell Culture for Quantitative Metabolomics

Multicellular 3D Models to Study Tumour-Stroma Interactions

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Production of Malignancy in Vitro. IV. The Mouse Fibroblast Cultures and Changes Seen in the Living Cells

Cited by 164 publications

References 0 publications

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Hydrogels for Bioprinting: A Systematic Review of Hydrogels Synthesis, Bioprinting Parameters, and Bioprinted Structures Behavior

Generation of 13C-Labeled Inositol and Inositol Phosphates by Stable Isotope Labeling Cell Culture for Quantitative Metabolomics

Multicellular 3D Models to Study Tumour-Stroma Interactions

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Product

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Generation of ¹³C-Labeled Inositol and Inositol Phosphates by Stable Isotope Labeling Cell Culture for Quantitative Metabolomics