Production of Lycopene in the Non-Carotenoid-Producing Yeast Yarrowia lipolytica

Matthäus, Falk; Ketelhot, Markus; Gatter, Michael; Barth, Gerold

doi:10.1128/aem.03167-13

Cited by 184 publications

(178 citation statements)

References 40 publications

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“…marginale (Pierlé et al 2013), R. pomeroyi (Green et al 2013), L. acidophilus (Askelson et al 2014), C. reinhardtir (Erpel et al 2016), Y. lipolytica (Matthaus et al 2014), S. elongates (van der Woude et al 2016) & baculovirus (Maghodia et al 2016). While it is encouraging that codon optimization software programs are used for a variety of species and purposes, most of the papers…”

Section: Acc E P Ted P R E P R I Ntmentioning

confidence: 99%

Synthetic gene design—The rationale for codon optimization and implications for molecular pharming in plants

Webster

Teh

K.‐C.

2016

Biotech & Bioengineering

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Degeneracy in the genetic code allows multiple codon sequences to encode the same protein. Codon usage bias in genes is the term given to the preferred use of particular synonymous codons. Synonymous codon substitutions had been regarded as "silent" as the primary structure of the protein was not affected; however, it is now accepted that synonymous substitutions can have a significant effect on heterologous protein expression. Codon optimization, the process of altering codons within the gene sequence to improve recombinant protein expression, has become widely practised.Multiple inter-linked factors affecting protein expression need to be taken into consideration when optimizing a gene sequence. Over the years, various computer programmes have been developed to aid in the gene sequence optimization process. However, as the rulebook for altering codon usage to affect protein expression is still not completely understood, it is difficult to predict which strategy, if any, will design the 'optimal' gene sequence.In this review, codon usage bias and factors affecting codon selection will be discussed and the evidence for codon optimization impact will be reviewed for recombinant protein expression using plants as a case study. These developments will be relevant to all recombinant expression systems, however, molecular pharming in plants is an area which has consistently encountered difficulties with low levels of recombinant protein expression, and should benefit from an evidence based rational approach to synthetic gene design. This article is protected by copyright. All rights reserved

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Section: Acc E P Ted P R E P R I Ntmentioning

confidence: 99%

Synthetic gene design—The rationale for codon optimization and implications for molecular pharming in plants

Webster

Teh

K.‐C.

2016

Biotech & Bioengineering

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“…[58] In addition, crtE gene or HMG1 gene encoding HMGR was overexpressed, enhancing lycopene production. [41,58,59] In other study, since the cytosol FPP pool partly flows into mitochondria, amorpha-4,11-diene synthase (ADS) was fused with mitochondrion-targeting signal peptide (mtADS) to utilize mitochondrial FPP as well ( Figure 6). [60] By coexpressing mtADS with the native ADS, amorphadiene production was greatly increased.…”

Section: Increasing Upstream Fluxmentioning

confidence: 99%

Metabolic Engineering of Microorganisms for the Production of Natural Compounds

Park

Yang

et al. 2017

Adv. Biosys.

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Natural products have been attracting much interest around the world for their diverse applications, especially in drug and food industries. Plants have been a major source of many different natural products. However, plants are affected by weather and environmental conditions and their successful extraction is rather limited. Chemical synthesis is inefficient due to the complexity of their chemical structures involving enantioselectivity and regioselectivity. For these reasons, an alternative means of overproducing valuable natural products using microorganisms has emerged. In recent years, various metabolic engineering strategies have been developed for the production of natural products by microorganisms. Here, the strategies taken to produce natural products are reviewed. For convenience, natural products are classified into four main categories: terpenoids, phenylpropanoids, polyketides, and alkaloids. For each product category, the strategies for establishing and rewiring the metabolic network for heterologous natural product biosynthesis, systems approaches undertaken to optimize production hosts, and the strategies for fermentation optimization are reviewed. Taken together, metabolic engineering has enabled microorganisms to serve as a prominent platform for natural compounds production. This article examines both the conventional and novel strategies of metabolic engineering, providing general strategies for complex natural compound production through the development of robust microbial‐cell factories.

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“…The purification of pigments from the yeast Phaffia rhodozyma was performed by chromatography using acetone and identification was performed by electronic absorption mass spectroscopy, confirming the synthesis of astaxanthin by the yeast (Johnson and Lewis, 1979). The production of lycopene by Yarrowia lipolytica was confirmed using HPLC with various compositions of the mobile phase, water, methanol, acetonitrile and ethyl acetate (Matthäus et al, 2014).…”

Section: Extraction Purification and Identification Of Carotenoidsmentioning

confidence: 94%

Microbial production of carotenoids A review

Cardoso¹,

Kanno²,

Karp³

2017

Afr. J. Biotechnol.

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Carotenoids are natural pigments that can be synthesized by various microorganisms, including bacteria, yeasts, filamentous fungi and microalgae. These pigments comprise around 700 different structures with peculiar colors and biological properties that are beneficial to health. Advantages of biotechnological production of carotenoids include the ability of microorganisms to use low cost substrates, the optimized control of cultivation, minimized production time and the natural origin of the synthesized pigments. Techniques for separation and purification of carotenoids are well established at laboratory scale, however the development of processes that can be economically scaled-up is essential for industrial production.

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Production of Lycopene in the Non-Carotenoid-Producing Yeast Yarrowia lipolytica

Cited by 184 publications

References 40 publications

Synthetic gene design—The rationale for codon optimization and implications for molecular pharming in plants

Synthetic gene design—The rationale for codon optimization and implications for molecular pharming in plants

Metabolic Engineering of Microorganisms for the Production of Natural Compounds

Microbial production of carotenoids A review

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