Production of low molecular weight chitosan by acid and oxidative pathways: Effect on physicochemical properties

Farias, Bruna Silva de; Gründmann, Dara Djenifer Rodrigues; Rizzi, Francisca Zuchoski; Martins, Natália Soares Santos; Cadaval, Tito R.S.; Pinto, Luiz Antônio de Almeida

doi:10.1016/j.foodres.2019.04.051

Cited by 62 publications

(23 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that the crystalline structure of chitosan is destroyed by ionic cross-linking interactions between the amino groups on chitosan and TPP [14,30]. Depolymerization reactions could have occurred favorably through the crystalline domains of the biopolymer, resulting in a decrease in the intensity of the characteristic peaks of chitosan [36]. Moreover, the d-spacing values of the N-acetyl-d-glucosamine portion and N-glucosamine portion were in agreement with previous reports [26,36].…”

Section: Crystalline Structure Of Chitosan and Chitosan Nanoparticlessupporting

confidence: 90%

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

2019

View full text Add to dashboard Cite

α- and β-Chitosan nanoparticles were obtained from shrimp shell and squid pen chitosan with different set of deacetylation degree (%DD) and molecular weight (MW) combinations. After nanoparticle formation via ionic gelation with sodium tripolyphosphate (TPP), the % crystallinity index (%CI) of the α- and β-chitosan nanoparticles were reduced to approximately 33% and 43% of the initial %CI of the corresponding α- and β-chitosan raw samples, respectively. Both forms of chitosan and chitosan nanoparticles scavenged superoxide radicals in a dose-dependent manner. The %CI of α- and β-chitosan and chitosan nanoparticles was significantly negatively correlated with superoxide radical scavenging abilities over the range of concentration (0.5, 1, 2 and 3 mg/mL) studied. High %DD, and low MW β-chitosan exhibited the highest superoxide radical scavenging activity (p < 0.05). α- and β-Chitosan nanoparticles prepared from high %DD and low MW chitosan demonstrated the highest abilities to scavenge superoxide radicals at 2.0–3.0 mg/mL (p < 0.05), whereas α-chitosan nanoparticles, with the lowest %CI, and smallest particle size (p < 0.05), prepared from medium %DD, and medium MW chitosan showed the highest abilities to scavenge superoxide radicals at 0.5–1.0 mg/mL (p < 0.05). It could be concluded that α- and β-chitosan nanoparticles had superior superoxide radical scavenging abilities than raw chitosan samples.

show abstract

Section: Crystalline Structure Of Chitosan and Chitosan Nanoparticlessupporting

confidence: 90%

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

2019

View full text Add to dashboard Cite

show abstract

“…Compared with HMW-CS, low molecular weight chitosan (LMW-CS) showed many advantages in CS-NPs production, such as better water solubility, smaller size, and higher drug loading. Thus far, LMW-CS was produced by several methods: physical degradation method (microwave and ultrasonication), chemical degradation method (acidic and alkaline treatment), and enzymatic method [26,27]. In contrast to the physical and chemical methods, enzymatic production method is advantageous in moderate reaction conditions, a lower risk of environmental pollution and easily-controlled product [28].…”

Section: Resultsmentioning

confidence: 99%

Enhancing the Thermo-Stability and Anti-Biofilm Activity of Alginate Lyase by Immobilization on Low Molecular Weight Chitosan Nanoparticles

Wang

et al. 2019

IJMS

View full text Add to dashboard Cite

Bacterial biofilm causes severe antibiotic resistance. An extracellular polymeric substance (EPS) is the main component in the bacterial biofilm. Alginate is a key EPS component in the biofilm of Pseudomonas aeruginosa and responsible for surface adhesion and stabilization of biofilm. Alginate lyase has emerged as an efficient therapeutic strategy targeting to degrade the alginate in the biofilm of P. aeruginosa. However, the application of this enzyme is limited by its poor stability. In this study, chitosan nanoparticles (CS-NPs) were synthesized using low molecular weight chitosan and alginate lyase Aly08 was immobilized on low molecular weight chitosan nanoparticles (AL-LMW-CS-NPs). As a result, the immobilization significantly enhanced the thermal stability and reusability of Aly08. In addition, compared with free Aly08, the immobilized AL-LMW-CS-NPs exhibited higher efficiency in inhibiting biofilm formation and interrupting the established mature biofilm of P. aeruginosa, which could reduce its biomass and thickness confirmed by confocal microscopy. Moreover, the biofilm disruption greatly increased the antibiotic sensitivity of P. aeruginosa. This research will contribute to the further development of alginate lyase as an anti-biofilm agent.

show abstract

“…Chitosan can be obtained by partial or total deacetylation of chitin, acetyl groups in the molecular chain of chitin being removed to form amino groups in chitosan. For this reason, chitosan can be classified as a copolymer composed mainly of 2-amino-2-deoxy-β- d -glucopyranose (glucosamine) and 2-acetamide-2-deoxy-β- d -glucopyranose units (N-acetylglucosamine) linked by glycosidic β(1→4) bonds [ 2 , 3 , 4 , 5 , 6 ] ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

Factors Influencing the Antibacterial Activity of Chitosan and Chitosan Modified by Functionalization

Ardean

Davidescu

Nemeş

et al. 2021

IJMS

221

View full text Add to dashboard Cite

The biomedical and therapeutic importance of chitosan and chitosan derivatives is the subject of interdisciplinary research. In this analysis, we intended to consolidate some of the recent discoveries regarding the potential of chitosan and its derivatives to be used for biomedical and other purposes. Why chitosan? Because chitosan is a natural biopolymer that can be obtained from one of the most abundant polysaccharides in nature, which is chitin. Compared to other biopolymers, chitosan presents some advantages, such as accessibility, biocompatibility, biodegradability, and no toxicity, expressing significant antibacterial potential. In addition, through chemical processes, a high number of chitosan derivatives can be obtained with many possibilities for use. The presence of several types of functional groups in the structure of the polymer and the fact that it has cationic properties are determinant for the increased reactive properties of chitosan. We analyzed the intrinsic properties of chitosan in relation to its source: the molecular mass, the degree of deacetylation, and polymerization. We also studied the most important extrinsic factors responsible for different properties of chitosan, such as the type of bacteria on which chitosan is active. In addition, some chitosan derivatives obtained by functionalization and some complexes formed by chitosan with various metallic ions were studied. The present research can be extended in order to analyze many other factors than those mentioned. Further in this paper were discussed the most important factors that influence the antibacterial effect of chitosan and its derivatives. The aim was to demonstrate that the bactericidal effect of chitosan depends on a number of very complex factors, their knowledge being essential to explain the role of each of them for the bactericidal activity of this biopolymer.

show abstract

Production of low molecular weight chitosan by acid and oxidative pathways: Effect on physicochemical properties

Cited by 62 publications

References 45 publications

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

Impact of Crystalline Structural Differences Between α- and β-Chitosan on Their Nanoparticle Formation Via Ionic Gelation and Superoxide Radical Scavenging Activities

Enhancing the Thermo-Stability and Anti-Biofilm Activity of Alginate Lyase by Immobilization on Low Molecular Weight Chitosan Nanoparticles

Factors Influencing the Antibacterial Activity of Chitosan and Chitosan Modified by Functionalization

Contact Info

Product

Resources

About