Production of Lovastatin using Liquid Cheese Whey by Fusarium nectrioides (MH173849), an Endophytic Fungi Isolated from Euphorbia hirta

Abstract

Lovastatin is a naturally produced 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase enzyme inhibitor- used for treating hypercholesterolemia. It was the first statin drug which was approved by the United States Food and Drug Administration (USFDA). In the current study, endophytic fungus Fusarium nectrioides (MH173849) isolated from Euphorbia hirta L. was used for the production of lovastatin. Four different culture media indicated as M1, M2, M3 and M4 were used for the initial production of lovastatin. Liquid cheese whey was used as nitrogen source. Growth morphology of fungi was investigated using Scanning Electron Microscopy analysis. Also, parameters like temperature, pH, inoculum size, incubation time, and RPM were optimized for the obtaining highest lovastatin production. Among the four media, M4 was found to produce the maximum concentration of lovastatin. Parameters such as temperature of 28°C, pH 6, RPM – 180 rpm and inoculum size of 5 x107 spores/mL were optimal for the production of lovastatin by F. nectrioides (MH173849).