Production of lipids with Microchloropsis salina in open thin-layer cascade photobioreactors

Schädler, Torben; Cerbon, D. Caballero; Oliveira, Luis; Garbe, Daniel; Brück, Thomas; Weuster‐Botz, Dirk

doi:10.1016/j.biortech.2019.121682

Cited by 23 publications

(36 citation statements)

References 34 publications

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“…Microchloropsis gaditana , previously known as Nannochloropsis gaditana , has great potential as industrial strain due to its higher lipid content ( Poliner et al., 2018 ; Schädler et al., 2019 ) (>50% dcw) and are also capable of converting atmospheric CO 2 into biomass ( Carlozzi, 2003 ), biofuels ( Mujtaba et al., 2012 ), and biorenewables precursors ( Fawley et al., 2015 ; Lee and Sun, 2019 ). Studies have shown that increase in biomass productivities can be achieved by enhancing photosynthetic efficiency through supplementation of CO 2 in Nannochloropsis sp.…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

et al. 2020

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Photosynthetic organisms fix inorganic carbon through carbon capture machinery (CCM) that regulates the assimilation and accumulation of carbon around ribulose-1,5bisphosphate carboxylase/oxygenase (Rubisco). However, few constraints that govern the central carbon metabolism are regulated by the carbon capture and partitioning machinery. In order to divert the cellular metabolism toward lipids and/or biorenewables it is important to investigate and understand the molecular mechanisms of the CO 2-driven carbon partitioning. In this context, strategies for enhancement of CO 2 fixation which will increase the overall biomass and lipid yields, can provide clues on understanding the carbon assimilation pathway, and may lead to new targets for genetic engineering in microalgae. In the present study, we have focused on the physiological and metabolomic response occurring within marine oleaginous microalgae Microchloropsis gaditana NIES 2587, under the influence of very-low CO 2 (VLC; 300 ppm, or 0.03%) and high CO 2 (HC; 30,000 ppm, or 3% v/v). Our results demonstrate that HC supplementation in M. gaditana channelizes the carbon flux toward the production of long chain polyunsaturated fatty acids (LC-PUFAs) and also increases the overall biomass productivities (up to 2.0 fold). Also, the qualitative metabolomics has identified nearly 31 essential metabolites, among which there is a significant fold change observed in accumulation of sugars and alcohols such as galactose and phytol in VLC as compared to HC. In conclusion, our focus is to understand the entire carbon partitioning and metabolic regulation within these photosynthetic cell factories, which will be further evaluated through multiomics approach for enhanced productivities of biomass, biofuels, and bioproducts (B3).

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Section: Introductionmentioning

confidence: 99%

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

et al. 2020

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“…) have previously been reported for Microchloropsis salina under nitrogen replete conditions (Gu et al 2022;Schädler et al 2019).…”

Section: Introductionmentioning

confidence: 63%

Simultaneous photoautotrophic production of DHA and EPA by Tisochrysis lutea and Microchloropsis salina in co-culture

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Gerwald

et al. 2022

Bioresour. Bioprocess.

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Marine microalgae have received much attention as a sustainable source of the two health beneficial omega-3-fatty acids docosahexaenoic acid (DHA, C22:6) and eicosapentaenoic acid (EPA, C20:5). However, photoautotrophic monocultures of microalgae can only produce either DHA or EPA enriched biomass. An alternative may be the photoautotrophic co-cultivation of Tisochrysis lutea as DHA-producer with Microchloropsis salina for simultaneous EPA production to obtain EPA- and DHA-rich microalgae biomass in a nutritionally balanced ratio. Photoautotrophic co-cultivation processes of T. lutea and M. salina were studied, applying scalable and fully controlled lab-scale gas-lift flat-plate photobioreactors with LED illumination for dynamic climate simulation of a repeated sunny summer day in Australia [day–night cycles of incident light (PAR) and temperature]. Monocultures of both marine microalgae were used as reference batch processes. Differences in the autofluorescence of both microalgae enabled the individual measurement, of cell distributions in co-culture, by flow cytometry. The co-cultivation of T. lutea and M. salina in artificial sea water with an inoculation ratio of 1:3 resulted in a balanced biomass production of both microalgae simultaneously with a DHA:EPA ratio of almost 1:1 (26 mgDHA gCDW−1, and 23 mgEPA gCDW−1, respectively) at harvest after depletion of the initially added fertilizer. Surprisingly, more microalgae biomass was produced within 8 days in co-cultivation with an increase in the cell dry weight (CDW) concentration by 31%, compared to the monocultures with the same amount of light and fertilizer. What is more, DHA-content of the microalgae biomass was enhanced by 33% in the co-culture, whereas EPA-content remained unchanged compared to the monocultures. Graphical Abstract

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“…Microalgae have been intensively studied as a sustainable source of various biobased products and they are especially attractive because of their efficiency in carbon sequestration and fixation, and their much higher growth yields and productivities compared to terrestrial plants (Schädler et al 2019). Microalgal biomass and metabolites have been studied and applied as sources of both high and low value compounds including proteins, carbohydrates, fatty acids, pigments such as carotenoids and phycocyanin, and their applications spread across the feeds and food, pharmaceuticals and energy industries (Mobin et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Developing food waste biorefinery: using optimized inclined thin layer pond to overcome constraints of microalgal biomass production on food waste digestate

et al. 2022

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Diversion of food waste from landfill through anaerobic digestion is a sustainable form of energy production (biogas) and the waste effluent (digestate) can be utilised as nutrient supply for microalgae cultivation. However, digestate has very high nutrient concentrations and is highly turbid, making it difficult to utilize as a nutrient source with conventional microalgae cultivation systems. Here we compared the efficiencies of a conventional open raceway pond (ORWP) and an improved inclined thin layer photobioreactor (ITLP) for the utilization and treatment of food waste derived digestate by Chlorella sp. The ITLP improved on volumetric and areal productivities by 17 and 3 times over the ORWP, with values of 0.563 and 31.916 g m −2 day −1 respectively. Areal nutrient removal via microalgae biomass were 2359.759 ± 64.75 and 260.815 ± 7.16 mg m −2 day −1 for nitrogen and phosphorous respectively in the ITLP, which are 2.8 times higher than obtained in the ORWP. The ITLP’s superiority stems from its ability to support a much higher average biomass yield of 6.807 g L −1, which was 7 times higher than in the ORWP. Mean irradiance in-situ was higher in the ITLP, irradiance distribution and utilization by the culture in the ITLP was 44% more efficient than in the ORWP. Our results indicate that the ITLP is a far more productive system than conventional raceway ponds. This demonstrates that integration of ITLP microalgae cultivation using digestate has the potential to make digestate management yield net benefit in food waste biorefinery settings.

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Production of lipids with Microchloropsis salina in open thin-layer cascade photobioreactors

Cited by 23 publications

References 34 publications

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

Photosynthetic Carbon Partitioning and Metabolic Regulation in Response to Very-Low and High CO2 in Microchloropsis gaditana NIES 2587

Simultaneous photoautotrophic production of DHA and EPA by Tisochrysis lutea and Microchloropsis salina in co-culture

Developing food waste biorefinery: using optimized inclined thin layer pond to overcome constraints of microalgal biomass production on food waste digestate

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