Production of Ligninolytic Enzymes by Cultures of White Rot Fungi

Abstract

Some Basidiomycota were chosen for studies of key ligninases synthesis (25°C, 30 days) in modified medium (shaken or not cultures) with added wheat straw. Liquid Czapek medium with straw yielded a higher amount of laccase than peroxidase, ground straw induced enzyme worse than chopped straw. With peroxidase the reverse dependencies were observed. Laccase of Lentinus edodes synthesized two enzyme isoforms (ca 30 and 16 kDa). In T. versicolor culture active laccase protein with highest molecular mass ca 65 kDa was found. P. sajor-caju yielded three different peroxidase isoforms. Ligninase biosynthesis depended on strain, straw fragmentation extent, culture method and growth medium.

Potential of ligninolytic enzymatic complex produced by white‐rot fungi from genus Trametes isolated from Bulgarian forest soil

“…One of the most effective approaches to increase the yield is the supplementation of the nutrient medium with an appropriate inducer [45,52]. Different natural or synthetic agents have been used to induce the synthesis of ligninases [11,13,15,43]. This study confirms published data showing that supplemental Tween 80, veratryl alcohol and glycerol cause an increase in Lac and MnP activity in extracellular fluid of T. trogii.…”

“…A negative effect of agitation of fungal cultures on Lac production has been reported also by other authors [47][48][49]. This effect could be attributed to mechanical damage to the mycelium during agitation and its harmful effect on fungal growth and synthesis of enzymes as well as to changes in CO 2 concentration in the medium [13,50].…”

“…This becomes especially evident for Lac production. Górska et al [13] also suggested that the average biosynthesis of Lac by fungal strains belonging to genera Pleurotus, Lentinus, Trametes and Phanerochaete in unshaken cultures was higher than in shaken cultures. A negative effect of agitation of fungal cultures on Lac production has been reported also by other authors [47][48][49].…”

“…Among all investigated fungi, white-rot basidiomycetes are known as the most effective producers of Lac, MnP and LiP [7,8,[11][12][13] that are synthesized during their secondary metabolism [14]. Considerable attention is focused on Phanerochaete chrysosporium, Phlebia radiata, Coriolus versicolor, Cyathus stercoreus, Ceriporiopsis subvermispora, Trametes versicolor, T. trogii, Pleurotus ostreatus, P. pulmonarius, Schizophyllum commune, etc.…”

“…In most cases, the synthesis of these three ligninolytic enzymes is differently affected by the cultural culture conditions [11,15,18]. Moreover, even among known producers, the enzyme levels are very low and various physicochemical parameters need to be optimized [13]. MnP production by the white-rot fungus P. chrysosporium BKM-F-1767 showed a significant dependence on some activators added to the nutrient medium (Mn 2+ , Tween 80, phenylmethylsulphonylfloride) [20].…”