Production of lignin modifying enzymes on industrial waste material by solid-state cultivation of fungi

Winquist, Erika; Moilanen, Ulla; Mettälä, Aila; Leisola, Matti; Hatakka, Annele

doi:10.1016/j.bej.2008.06.006

Cited by 63 publications

(31 citation statements)

References 35 publications

(37 reference statements)

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“…Solid state cultivation on lignocellulose is often suitable for the production of LiP and MnP [42], but it was not superior in our study. However, the enzyme activities in the extracts are difficult to directly compare with the liquid culture supernatants because the mycelial growth is different in solid medium but also because the activity was calculated on the basis of liquid extracted, here 4 g oat husk medium extracted with 16 ml buffer [35], not on the culture filtrates of liquid cultures, which contain less but more easily digestible carbon sources. The use of more nutrient rich media in screening allowed the expression of lignin-modifying peroxidases in many fungi, but apparently the production of LiP was promoted by nutrient poor medium.…”

Section: Production Of Lignin-modifying Peroxidasesmentioning

confidence: 99%

“…The media were a) low nitrogen (2 mM) asparagine-dimethyl succinate-medium (M), containing 0.5% (w/v) glucose, pH 4.5 [23], b) peptone medium (P) with yeast extract 0.2% (w/v), peptone 0.5% (w/v), glucose 2% (w/v), 7 mM KH2PO4, 2 mM MgSO4 and 100 µM MnSO4, pH 5 (modified from [34]), c) soy medium (S) with 2% (w/v) soy briquettes (Mildola Ltd, Kirkkonummi, Finland), water, pH adjusted to 5, and d) solid oat husk (Rapion Tuote Inc., Finland) medium (O) with water supplementation [35]. For the production of inocula fungi were cultivated in 2% (w/v) malt extract medium and the mycelium with the medium was homogenized in a Waring blender four times 10 sec in 30 sec intervals.…”

Section: Culture Conditionsmentioning

confidence: 99%

“…Samples from solid state cultivations were extracted in a ratio liquid: solids = 6:1 (4 g milled, to pass 2 mm sieve, oat husks humified by 8 ml water and extracted by 16 ml 0.05 M sodium phosphate buffer, pH 6.5) as described previously [35]. The culture filtrates and extracts were centrifuged for 2 min 16,000*g and 50 μl supernatants were used for enzyme assays.…”

Section: Culture Conditionsmentioning

confidence: 99%

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Improved Efficiency in Screening for Lignin-Modifying Peroxidases and Laccases of Basidiomycetes

Kinnunen

Maijala

Järvinen

et al. 2017

CBIOT

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Abstract:Background: Wood rotting white-rot and litter-decomposing basidiomycetes form a huge reservoir of oxidative enzymes, needed for applications in the pulp and paper and textile industries and for bioremediation.Objective: The aim was (i) to achieve higher throughput in enzyme screening through miniaturization and automatization of the activity assays, and (ii) to discover fungi which produce efficient oxidoreductases for industrial purposes.Methods: Miniaturized activity assays mostly using dyes as substrate were carried out for lignin peroxidase, versatile peroxidase, manganese peroxidase and laccase. Methods were validated and 53 species of basidiomycetes were screened for lignin modifying enzymes when cultivated in liquid mineral, soy, peptone and solid state oat husk medium.Results: Manganese peroxidases were the most common enzymes produced by 96% of the species. They typically had acidic pH optima, although Hyphodontia sp., Pleurotus pulmonarius and Trametes ochracea produced enzymes highly active at pH 7. Versatile peroxidase was produced by 66% of the fungi with efficient production from Phlebia radiata, P. pulmonarius and Galerina marginata. Novel lignin peroxidase producing fungi Cylindrobasidium evolvens and Daedaleopsis septentrionalis were found among the 26% of the species showing here lignin peroxidase production. Laccase was shown in 92% of the species. Several fungi produced laccase active at pH 7, which is noteworthy because usually laccases of white-rot fungi are efficient and relevant for many industrial applications. Conclusion:Automated screening allowed us to monitor many specific enzyme activities and extend the range of assay conditions from relatively small fungal cultivation sample volumes.

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Section: Production Of Lignin-modifying Peroxidasesmentioning

confidence: 99%

Section: Culture Conditionsmentioning

confidence: 99%

Section: Culture Conditionsmentioning

confidence: 99%

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Improved Efficiency in Screening for Lignin-Modifying Peroxidases and Laccases of Basidiomycetes

Kinnunen

Maijala

Järvinen

et al. 2017

CBIOT

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“…evolution from 14 C-[Ring]-labelled synthetic lignin (DHP) (13,35). In order to depolymerize and mineralize lignin, they have developed an oxidative and unspecific system including extracellular enzymes, low molecular weight metabolites and activated oxygen species (28,29).…”

Section: Co2mentioning

confidence: 99%

Screening for ligninolytic enzymes from autochthonous fungi and applications for decolorization of Remazole Marine Blue

Erden¹,

Ucar²,

Gezer³

et al. 2009

Braz. J. Microbiol.

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This study presents new and alternative fungal strains for the production of ligninolytic enzymes which have great potential to use in industrial and biotechnological processes. Thirty autochthonous fungal strains were harvested from Bornova-Izmir in Turkiye. In the fresh fruitbody extracts laccase, manganese peroxidase and lignin peroxidase activities, which are the principal enzymes responsible for ligninocellulose degradation by Basidiomycetes, were screened. Spores of some of the basidiomycetes species such as Cortinarius sp., Trametes versicolor, Pleurotus ostreatus, Abortiporus biennis, Lyophyllum subglobisporium, Ramaria stricta, Ganoderma carnosum, Lactarius delicious ve Lepista nuda were isolated and investigated optimum cultivation conditions in submerged fermentation for high yields of ligninolytic enzyme production. In addition, isolated fungal strains were monitored on agar plates whether having the capability of decolorization of a textile dye Remazol Marine Blue.

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“…Despite relatively long incubation times, SSF offers an inexpensive and effective means of fungal cultivation that can also be used for the production of potentially valuable fungal enzymes [79][80][81]. Fungal enzymes produced by SSF have been used to enhance methane production by anaerobic digestion [82].…”

Section: Species and Systems Investigatedmentioning

confidence: 99%

Pretreatment of Lignocellulosic Biomass Using Microorganisms: Approaches, Advantages, and Limitations

Canam¹,

Town²,

Iroba³

et al. 2013

Sustainable Degradation of Lignocellulosic Biomass - Techniques, Applications and Commercialization

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Additional information is available at the end of the chapter http://dx.doi.org/10.5772/55088 . IntroductionMuch of Earth s recent geologic history is dominated by periods of extensive glaciation, with relatively low global mean temperatures and correspondingly low atmospheric CO concentrations [ ]. The current interglacial period stands out as an anomaly because the atmospheric CO concentration has risen sharply above the range of approximately -parts per million by volume that has defined the past , years to reach levels that are nearly % higher than the biosphere has experienced over this time frame [ ]. This rapid increase in CO concentration is primarily due to the release of ancient fixed atmospheric CO into the modern atmosphere through the combustion of fossil fuel resources over the past years. Since it is clear from ice core records that atmospheric CO concentration has a strong positive correlation to global temperature, it is expected that changes to global climate are forthcoming [ ]. There are substantial uncertainties regarding the ability of terrestrial and oceanic carbon sinks to absorb this anthropogenic CO on time scales that are relevant to human society [ ], so the continued release of ancient CO into the modern atmosphere at current rates carries with it an important risk of inducing climate changes of unknown amplitude along with a host of ancillary changes that are difficult to predict with certainty. This has led to the search for alternatives to fossil fuels to meet a rising global energy demand, and one such option is the use of extant organic matter to produce energy. This resource contains carbon that was fixed from the modern atmosphere, which means it does not result in a net increase in atmospheric CO upon combustion.

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Production of lignin modifying enzymes on industrial waste material by solid-state cultivation of fungi

Cited by 63 publications

References 35 publications

Improved Efficiency in Screening for Lignin-Modifying Peroxidases and Laccases of Basidiomycetes

Improved Efficiency in Screening for Lignin-Modifying Peroxidases and Laccases of Basidiomycetes

Screening for ligninolytic enzymes from autochthonous fungi and applications for decolorization of Remazole Marine Blue

Pretreatment of Lignocellulosic Biomass Using Microorganisms: Approaches, Advantages, and Limitations

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