Production of levansucrase from novel honey Bacillus subtilis isolates capable of producing antiviral levans

Esawy, Mona A.; Ahmed, Eman F.; Helmy, Wafaa A.; Mansour, Nabil; El‐Senousy, Waled M.; El-Safty, Mounir M.

doi:10.1016/j.carbpol.2011.05.035

Cited by 103 publications

(60 citation statements)

References 31 publications

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“…are the most predominant microorganism in honey; they possess spores and have the ability to withstand the characteristic nature of honey (Nevas et al, 2006). A study carried out by Esawy et al (2011Esawy et al ( , 2012 reported a novel Bacillus subtilis in honey isolate as a new source of very important enzymes such as levansucrase, dextranase and lipase. Bacillus strain Mori 2 has been isolated from honey.…”

Section: Honey Is a Sweet Viscous Liquid Produced By Honey Beementioning

confidence: 99%

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Antibacterial potential components of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria

Agbagwa¹,

Rani²,

Halami³

2017

Afr. J. Biotechnol.

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Honey is a sweet viscous liquid produced by honey bee, Apis mellifera from the nectar of plants. Honey is a natural product that has been used from ancient times till now as food and for medicinal purpose. This study was carried out to determine the mode of action of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria. Bacilli spp. count was carried out by initially heating diluted honey samples in water bath set at 80°C for 15 min, while total bacterial count was carried out using the pour plate method. Antibacterial activity of identified Bacillus spp on Micrococcus was determined using well-in agar method while the mode of action was carried out by reporter assay method. Detection of tetracycline, gentamycin and streptomycin was analyzed using high performance liquid chromatography (HPLC) column oven L-2300 and Column intensil ODS-3C18 (250 x 4.6 mm). Honey samples (2 g) were extracted for HPLC by deprotenizing using acetonitrile and methanol with flow rate of 1 mL/min and RID detector was used to detect antibiotic residue. Bacilli from honey were characterized physiologically, morphologically and biochemically, they were tentatively identified as Bacilli licheniformis, Bacilli subtilis, Bacilli coagulans, Bacilli cirulans, Bacilli pumilis and Bacilli badius. The most prevalent Bacillus spp. were B. licheniformis and B. subtilis. Total bacteria count for branded honey ranged from 2.2 x 10 2 to 5.5 x 10 3 cfu/g, while Bacilli count ranged from nil to 6.2 x 10 2 cfu/g. For unbranded honey samples, total bacteria count ranged from 7.0 x 10 3 to 3.5 x 10 2 cfu/g, while Bacilli count ranged from 5 x 10 1 to 1.6 x 10 3 cfu/g. Four of the isolates representing branded (SF2 and RW2) and unbranded honey samples (EH2 and TC2) exhibited antibacterial activity against Micrococcus; one isolate (SF2) showed cell wall causing antibacterial activity. Tetracycline was detected more in the unbranded honey samples while gentamycin and streptomycin were detected in just two unbranded honey samples, indicating that tetracycline is used frequently for the treatment of bee diseases that is why it is detected as residue in the finished honey product.

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Section: Honey Is a Sweet Viscous Liquid Produced By Honey Beementioning

confidence: 99%

“…Recent studies confirmed that Bacillus spp. from honey can be used for production of important enzymes such as levansucrase and other important enzymes (Esawy et al, 2011(Esawy et al, , 2012Safty, 2011).…”

Section: Enzyme Assaymentioning

confidence: 99%

Antibacterial potential components of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria

Agbagwa¹,

Rani²,

Halami³

2017

Afr. J. Biotechnol.

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“…Honey osmophilic property commended it to be good medium for dominant spores worthy to produce bio-products with unique properties (Esawy et al, 2011;Esawy et al, 2012;Esawy et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Sequential optimizations of Aspergillus awamori EM66 exochitinase and its application as biopesticide

Awad¹,

Wahab²,

Hussein³

et al. 2017

J App Pharma Sci

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Sequential optimization strategy was achieved based on statistical experimental designs for enhancement of Aspergillus awamori EM66 exochitinase production. Firstly, 2-level Plackett-Burman design was accomplished for distinguishing between the bioprocess parameters, which improve the exochitinase production. Second optimization step was implemented through central composite design (CCD), for optimization variables amounts which have the highest positive effect on exochitinase production. Maximum activity (5998mU/ml) for exochitinase reported approximately 22 fold increase compared to the basal medium activity. Mortality 92%, 86.67% and 65.67% was recorded when the partial pure fungal exochitinase was applied to the diet of the greater wax moth larvae, Galleria mellonella, the cotton leaf worm, Spodoptera littoralis, and the black cutworm, Agrotis ipsilon, respectively. The results indicated to the importance of Aspergillus awamori EM66 exochitinase as an effective bio-pesticide.

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“…This expectation comes from the honey constituents which is mainly fructose (about 38.5%) and glucose (about 31.0%) (Crosby and Alfred 2004). Aerobic spore forming Bacillus were the most frequently encountered microbes on the external surface, crop and intestine of the honey bees and consequently honey (Root, 1993, Esawy et al, 2011.…”

Section: Introductionmentioning

confidence: 99%

“…None till now paid attention to the enzymatic products of these dormant endospores (Esawy et al, 2011). Osmophilic microorganisms survive environmental extremes of desiccation, pressure and acidity, it is expected that their biopolymers will also have some unique properties to adapt to such extreme conditions.…”

Section: Introductionmentioning

confidence: 99%

Antiviral Levans from Bacillus spp. Isolated from Honey

Esawy¹,

Ahmed²,

Helmy³

et al. 2012

The Complex World of Polysaccharides

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Production of levansucrase from novel honey Bacillus subtilis isolates capable of producing antiviral levans

Cited by 103 publications

References 31 publications

Antibacterial potential components of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria

Antibacterial potential components of Bacillus species and antibiotics residues in branded and unbranded honey samples from Nigeria

Sequential optimizations of Aspergillus awamori EM66 exochitinase and its application as biopesticide

Antiviral Levans from Bacillus spp. Isolated from Honey

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