Production of L-Asparaginase, an Anticancer Agent, From Aspergillus niger Using Agricultural Waste in Solid State Fermentation

Abstract

This article reports the production of high levels of L-asparaginase from a new isolate of Aspergillus niger in solid state fermentation (SSF) using agro-wastes from three leguminous crops (bran of Cajanus cajan, Phaseolus mungo, and Glycine max). When used as the sole source for growth in SSF, bran of G. max showed maximum enzyme production followed by that of P. mungo and C. cajan. A 96-h fermentation time under aerobic condition with moisture content of 70%, 30 min of cooking time and 1205-1405 micro range of particle size in SSF appeared optimal for enzyme production. Enzyme yield was maximum (40.9 +/- 3.35 U/g of dry substrate) at pH 6.5 and temperature 30 +/- 2 degrees C. The optimum temperature and pH for enzyme activity were 40 degrees C and 6.5, respectively. The study suggests that choosing an appropriate substrate when coupled with process level optimization improves enzyme production markedly. Developing an asparaginase production process based on bran of G. max as a substrate in SSF is economically attractive as it is a cheap and readily available raw material in agriculture-based countries.

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

“…SSF offers many advantages over SmF such as lower energy requirements, less risk of bacterial contamination, less waste water generation and less environmental concerns regarding the disposal of solid waste (Doelle et al, 1992). L-Asparaginase production in SSF has been reported earlier on wheat bran , soya bean meal (Bessoumy et al, 2004) and wastes from three leguminous crops-bran of Cajanus cajan, Phaseolus mungo and Glycine max (Mishra, 2006).…”

Anticancer Activity of L-Asparaginase Produced from Vigna Unguiculata

“…The enzyme activity was increased to 490 U/ml at this temperature. Moreover, L-asparaginase isolated from most organisms has maximum activity at 37°C [37,46,58]. The increase or decrease in reaction temperature above or below the optimum temperature cause a decrease in enzyme activity.…”

“…Currently, the most commonly use cancer chemotherapy includes mainly alkylating agents, antimetabolites and natural anticancer agents [23,[34][35][36]. The microbial L-asparaginaseshave only been produced commercially as a drug in the treatment of acute lymphoblastic leukemia [21,37,38]. Different studies [22,39] discovered plant natural products have more toxic to cancer cells than normal cells in screening and developed chemotherapy drugs [27,28,35,40].…”

Production, Purification and Standardization of Anticancerous Enzyme (L-Asparaginases) from Aspergillus Niger Using Soil Samples by Solid State Fermentation

“…Though L-Asparaginase has been reported in many higher plants, little work has been carried out on their purification and characterization. The L-asparaginases of Erwinia and E. coli have been reported since many years as effective drugs in the treatment of acute lymphoblastic leukemia (Mishra et al, 2006;Pieters et al, 2011). It has been observed that eukaryote microorganisms like yeast and filamentous fungi also have a potential for Asparaginase production (Wade et al, 1971).…”