Production of inulinase by a new mold of Penicillium rugulosum

Barthomeuf, Chantal; Regerat, F; Pourrat, Henri

doi:10.1016/0922-338x(91)90063-m

Cited by 45 publications

(26 citation statements)

References 18 publications

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“…Separation of fructose from this high fructose corn syrup is costly and thus makes this method uneconomical. Fructose can also be obtained by acid hydrolysis of the inulin, but fructose is easily degraded at low pHs and the process gives rise to coloring of the inulin hydrolysate and byproduct formation in the form of difructose anhydrides (Vandamme & Derycke 1983;Barthomeuf et al 1991). Another source includes microorganisms, some of which secrete high amounts of exoinulinase (fructanase, EC 3.2.1.80), which can cleave fructose from inulin (a polymer of fructose with terminal glucose), by a single step catalyzed reaction with yields up to 95% of fructose (Kaur et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Improvement in Inulinase Production by Simultaneous Action of Physical and Chemical Mutagenesis in Penicillium purpurogenum

Sharma

Gill

Bhullar

et al. 2005

World J Microbiol Biotechnol

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Section: Introductionmentioning

confidence: 99%

Improvement in Inulinase Production by Simultaneous Action of Physical and Chemical Mutagenesis in Penicillium purpurogenum

Sharma

Gill

Bhullar

et al. 2005

World J Microbiol Biotechnol

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“…vinaceum and Thielavia terricola presented better development in the I medium (growth rate = 100%), thus considered as capable to hydrolyse inulin. Several Aspergillus and Penicillium species are known as inulinase producer (3,9,13,27,29,30,43,44). C. elegans, P. verruculosum, Pestalotiopsis guepinii and Rhizopus oryzae isolated from the Viguiera aff.…”

Section: Enzymatic Characterization Of Fungi Strains According To Thementioning

confidence: 99%

Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin

Souza-Motta

Cavalcanti

Fernandes

et al. 2003

Braz. J. Microbiol.

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Filamentous fungi able to hydrolyse inulin have been isolated from the rhizosphere of plants whose roots contain this polysaccharide. This study reports results concerning the isolation and identification of filamentous fungi from the soil used for sunflower cultivation and from the sunflower rhizosphere cultivated in field and in greenhouse. Fungi were evaluated according to their capacity to hydrolyse inulin and the variation in the diversity of these fungi during the plant's life cycle was also accessed. Forty-nine species of filamentous fungi were isolated. Penicillium and Aspergillus were the genera that presented higher number of species, nine and seven, respectively. At the end of the sunflower life cycle, cultivated both in field and in the greenhouse, a lower numbers of species were isolated. One hundred and fifty nine strains of filamentous fungi were isolated from soil and from the sunflower rhizosphere; from these, 79 (49.7%) were able to hydrolyse inulin. There was not significant difference in the proportion of species able to hydrolyse this polysaccharide during the sunflower's life cycle, in plants cultivated in field or in greenhouse. Although the sunflower's rhizosphere is a source of filamentous fungi able to hydrolyse inulin, that might be used in biotechnological processes. This system does not present a higher density of such microorganisms. Species of Aspergillus, Chaetomium, Cunninghamella, Emericela, Eupenicillium, Fusarium, Myrothecium, Neosartorya, Neocosmospora, Penicillium and Thielavia are being related by first time as inulinase producers.

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“…TN88 (Nakamura et al, 1997), Aspergillus species (Vandamme and Derycke, 1993;Nakamura et al, 1994;Ettalibi and Barotti, 1987) and Fusarium oxysporum (Kaur et al, 1992), yeasts, e.g., Kluyveromyces marxianus CDBB-L-278 (Guerrero et al, 1995), Kluyveromyces fragilis and Debaryomyces cantarelli (Barthomeuf et al, 1991), Candida kefyr (Negoro and Kito, 1973), and bacteria, e.g., Bacillus circulans (Kushibe et al, 1995), Clostridium acetobutylicum ABKn8 (Efstahion et al, 1986), Pseudomonas sp. (Kim et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Parameters optimization for enzymatic assays using experimental design

Burkert

Kalil

Filho

et al. 2006

Braz. J. Chem. Eng.

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-The conditions for maximization enzymatic activity were determined using experimental design and inulinase from Kluyveromyces marxianus ATCC 16045. The effects of substrate concentration (sucrose and inulin), pH and temperature on inulinase activity were verified using four factorial design and surface response analysis. Using sucrose as substrate. It has bean shown that the effects sucrose on enzymatic activity is not statistically significant and the best condition for the highest activity (110 U/mL) was achieved with temperature between 60°C and 68°C and pH between 4.5 and 5.0. Using inulin as substrate it was verified that temperature is the only variable statistically significant and the maximum activity was 7.3 U/mL at temperature between 50°C and 51°C.

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Production of inulinase by a new mold of Penicillium rugulosum

Cited by 45 publications

References 18 publications

Improvement in Inulinase Production by Simultaneous Action of Physical and Chemical Mutagenesis in Penicillium purpurogenum

Improvement in Inulinase Production by Simultaneous Action of Physical and Chemical Mutagenesis in Penicillium purpurogenum

Identification and characterization of filamentous fungi isolated from the sunflower (Helianthus annus L.) rhizosphere according to their capacity to hydrolyse inulin

Parameters optimization for enzymatic assays using experimental design

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