Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and ?Morita navel? orange (Citrus sinensis Osbeck)

Takami, Kayo; Matsumara, A; Yoshio, Masaki; Imayama, Teruyuki; Kunitake, Hisato; Komatsu, Hirokazu

doi:10.1007/s00299-004-0777-z

Cited by 11 publications

(7 citation statements)

References 14 publications

(18 reference statements)

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“…A morfologia dos híbridos simétricos interespecíficos e intergenéricos é, geralmente, intermediária a dos genitores. Características, como folhas mais espessas, arredondadas, normalmente mais escuras e com pecíolo alado maior ou intermediário aos parentais e glândulas de óleo aparentes na face abaxial, podem ser comparadas com os genitores para a comprovação da hibridação (Takami et al, 2004). Plantas com morfologia intermediária têm também sido derivadas de fusões assimétricas.…”

Section: Resultsunclassified

Regeneração de plantas após fusão de protoplastos de tangelo 'Page' e toranja 'Lau Tau'

et al. 2007

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RESUMO -Buscou-se a hibridação somática entre tangelo 'Page' e toranja 'Lau Tau' visando à produção de porta-enxerto semelhante à laranja-azeda, por esta espécie ser considerada um provável híbrido entre C. reticulata e C. grandis. Após isolamento, fusão e cultivo de protoplastos, obtiveram-se brotações que foram enxertadas in vitro, em laranja 'Hamlin'. Dezessete plantas foram aclimatizadas em casa de vegetação. A análise de citometria de fluxo confirmou a constituição diplóide dessas plantas. Marcadores moleculares RAPD das plantas regeneradas apresentaram padrão de bandas similar ao de tangelo 'Page'. Entretanto, todas as plantas apresentaram conformação fenotípica diferente dos genitores. Termos para indexação: citros, cultura de tecidos, hibridação somática, porta-enxerto, melhoramento genético. PLANT REGENERATION AFTER PROTOPLAST FUSION OF 'PAGE' TANGELO AND 'LAU TAU' PUMMELOABSTRACT -This work aimed to produce somatic hybrid between 'Page' tangelo and 'Lau Tau' pummelo in an attempt to regenerate a similar rootstock to sour orange, because this species is considered a probable hybrid between C. reticulata and C. grandis. After protoplast isolation, fusion and culture regenerated shoots were in vitro grafted on 'Hamlin' sweet orange. Seventeen plants were acclimatized in a greenhouse. Citometric flow analyses revealed that all plants are diploid. RAPD molecular markers of regenerated plants had the same pattern as compared to 'Page' tangelo. However, all plants had phenotypic traits different from both genitors.

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Section: Resultsunclassified

Regeneração de plantas após fusão de protoplastos de tangelo 'Page' e toranja 'Lau Tau'

et al. 2007

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“…But presence of mitochondrion from one fusion parent has been reported in some intergeneric somatic hybrids (Kirti et al, 1992a;Nakajo et al, 1994;Wolters et al, 1995;Liu et al, 2002b;Vasilenko et al, 2003;Xu et al, 2004;Takami et al, 2004). It is not clear why mitochondrial genomes undergo frequent recombination or rearrangement in the somatic hybrids.…”

Section: Inheritance Of Mitochondrial Genomementioning

confidence: 94%

Intergeneric somatic hybridization and its application to crop genetic improvement

Liu

Deng

2005

Plant Cell Tiss Organ Cult

112

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Related or distant species of cultivated crops are a large pool of many desirable genes. Gene transfer from these species through conventional breeding is difficult owing to post-and pre-zygotic sexual incompatibilities. Somatic hybridization via protoplast fusion is a possible alternative for gene transfer from these species to cultivated crops. Since the early days of somatic hybridization many intergeneric somatic hybrids have been developed through symmetric fusion, asymmetric fusion and microfusion. Somatic hybrids are mainly selected by using markers such as specific media or fusion parents with special features, biochemical mutants, antibiotic resistance and complementation strategy. The hybridity of the regenerants is determined based on morphological, cytological and molecular analysis. The inheritance patterns of nuclear and cytoplasmic genomes in the somatic hybrids are diverse. Nuclear DNA from both fusion parents co-exists congruously in some hybrids with translocation and rearrangement of chromosomes, but spontaneous elimination of chromosomes from either or both fusion parents has been observed very often. In asymmetric fusion, chromosome elimination is an important issue that is a complicated process influenced by many factors, such as irradiation dose, phylogenetic relatedness, ploidy level of fusion parent and regenerants. As for chloroplast genome, uniparental segregation is mainly detected, though co-existence is also reported in some cases. The mitochondrial genome, in contrast to chloroplast, undergoes recombination and very frequent rearrangements. Somatic cell fusion has potential applications for crop genetic improvement by overcoming sexual incompatibility or reproductive barriers, and by realizing novel combinations of nuclear and/or cytoplasmic genomes.

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“…were fused-targeting blight, Citrus tristeza virus, and Phytophthora-induced disease tolerance (Calixto et al, 2004). Intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and 'Morita' navel orange were produced to create novel parents for interploid crosses aimed to develop new seedless kumquatlike cultivars (Takami et al, 2004).…”

Section: Citrus Speciesmentioning

confidence: 99%

Application of somatic hybridization for the improvement of horticultural crops

Singh¹,

Mishra²,

Kumar³

et al. 2015

HBR

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Somatic hybridization (SH) using protoplast fusion is a promising tool to produce symmetrical and asymmetrical polyploidy somatic hybrids in many agricultural crops. The technique of SH could facilitate conventional breeding by providing novel lines so as to use them as elite breeding materials in conventional crosses for both scion and rootstock improvement. Further, SH can overcome those problems associated with sexual hybridization, viz., sexual incompatibility, nucellar embryogenesis, and male/female sterility. Successful exploitation of SH in horticultural crops mainly comes from the transfer of resistance genes for biotic and abiotic stresses from related species in several horticultural crops, viz., citrus, potato, brinjal, tomato, mango, avocado, banana, strawberry, pear, cherry, etc. Unlike transgenic technology, SH is not affected by legal formalities and able to transfer uncloned multiple genes. However, certain boundaries and limitations of SH restricts its use over sexual hybridization, but envisage of new genomic technologies providing better insight into the plant genomes will increase the potentiality of SH in the betterment of agriculture.

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Production of intergeneric somatic hybrids between round kumquat (Fortunella japonica Swingle) and ?Morita navel? orange (Citrus sinensis Osbeck)

Cited by 11 publications

References 14 publications

Regeneração de plantas após fusão de protoplastos de tangelo 'Page' e toranja 'Lau Tau'

Regeneração de plantas após fusão de protoplastos de tangelo 'Page' e toranja 'Lau Tau'

Intergeneric somatic hybridization and its application to crop genetic improvement

Application of somatic hybridization for the improvement of horticultural crops

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