Production of Immunoabsorbent Nanoparticles by Displaying Single‐Domain Protein A on Potato Virus X

Uhde‐Holzem, Kerstin; McBurney, Michael W.; Tiu, Brylee David B.; Advíncula, Rigoberto C.; Fischer, Rainer; Commandeur, Ulrich; Steinmetz, Nicole F.

doi:10.1002/mabi.201500280

Cited by 32 publications

(31 citation statements)

References 26 publications

(66 reference statements)

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“…The study elucidated that amino acid content, mainly tryptophan, and isoelectric point could drastically alter the trafficking of the PVX through plants. For biosensing and diagnostic applications, PVX was genetically altered to display protein A fragments from Staphylococcus aureus [87]. The protein A coated PVX was then readily functionalized with IgG and was applied in biosensing of plant viruses [88].…”

Section: Delivery Of Peptides and Protein Drugsmentioning

confidence: 99%

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Chung

Cai

Steinmetz

2020

Advanced Drug Delivery Reviews

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Viral nanoparticles (VNPs) encompass a diverse array of naturally occurring nanomaterials derived from plant viruses, bacteriophages, and mammalian viruses. The application and development of VNPs and their genomefree versions, the virus-like particles (VLPs), for nanomedicine is a rapidly growing. VLPs can encapsulate a wide range of active ingredients as well as be genetically or chemically conjugated to targeting ligands to achieve tissue specificity. VLPs are manufactured through scalable fermentation or molecular farming, and the materials are biocompatible and biodegradable. These properties have led to a wide range of applications, including cancer therapies, immunotherapies, vaccines, antimicrobial therapies, cardiovascular therapies, gene therapies, as well as imaging and theranostics. The use of VLPs as drug delivery agents is evolving, and sufficient research must continuously be undertaken to translate these therapies to the clinic. This review highlights some of the novel research efforts currently underway in the VNP drug delivery field in achieving this greater goal.

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Section: Delivery Of Peptides and Protein Drugsmentioning

confidence: 99%

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Chung

Cai

Steinmetz

2020

Advanced Drug Delivery Reviews

Self Cite

256

241

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“…As another approach, it was recently demonstrated that functionalization of PVX-based nanoparticles with protein A fragments can be used to display whole antibody molecules by using protein A's property of binding to the Fc region of the heavy chain of IgGs. 85 The PVX particles could then be used as a plug-and-play system for the display of a variety of antibodies, with a level of orientational control not achievable with chemical conjugation.…”

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

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“…For PVX, an alternative methodology to display a target protein is the insertion of the FMDV 2A sequence between the target gene and the cp , hence a ribosomal skip occurs during translation, leading to the expression of the CP fusion protein, free target protein as well as wild-type CP (Cruz et al, 1996; Donnelly et al, 2001a,b). The FMDV 2A sequence has previously been used in PVX vectors to avoid unfavorable limitations of CP fusions (Cruz et al, 1996; Lee et al, 2014; Shukla et al, 2014; Uhde-Holzem et al, 2015), but to our knowledge it has not yet been used in a TMV CP fusion protein.…”

Section: Introductionmentioning

confidence: 99%

Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display

2017

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Plant viruses are suitable as building blocks for nanomaterials and nanoparticles because they are easy to modify and can be expressed and purified using plants or heterologous expression systems. Plant virus nanoparticles have been utilized for epitope presentation in vaccines, for drug delivery, as nanospheres and nanowires, and for biomedical imaging applications. Fluorescent protein fusions have been instrumental for the tagging of plant virus particles. The monomeric non-oxygen-dependent fluorescent protein iLOV can be used as an alternative to green fluorescent protein. In this study, the iLOV sequence was genetically fused either directly or via a glycine-serine linker to the C-terminus of the Tobacco mosaic virus (TMV) coat protein (CP) and also carried an N-terminal Foot-and-mouth disease virus (FMDV) 2A sequence. Nicotiana benthamiana plants were inoculated with recombinant viral vectors and a systemic infection was achieved. The presence of iLOV fusion proteins and hybrid particles was confirmed by western blot analysis and transmission electron microscopy. Our data suggest that TMV-based vectors are suitable for the production of proteins at least as large as iLOV when combined with the FMDV 2A sequence. This approach allowed the simultaneous production of foreign proteins fused to the CP as well as free CP subunits.

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Production of Immunoabsorbent Nanoparticles by Displaying Single‐Domain Protein A on Potato Virus X

Cited by 32 publications

References 26 publications

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Design of virus-based nanomaterials for medicine, biotechnology, and energy

Adoption of the 2A Ribosomal Skip Principle to Tobacco Mosaic Virus for Peptide Display

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