Production of IL-27 and other IL-12 family cytokines by microglia and their subpopulations

Inhibition of midkine alleviates experimental autoimmune encephalomyelitis through the expansion of regulatory T cell population

“…Real-time RT-PCR analysis was carried out by using an ABI Prism 7000 sequence detection system (Applied Biosystems) as described previously (50). The relative expression levels of Foxp3 were determined by normalization to the GAPDH expression level.…”

Chronological Changes of CD4+ and CD8+ T Cell Subsets in the Experimental Autoimmune Encephalomyelitis, a Mouse Model of Multiple Sclerosis

“…RNA isolation and RT were performed as described previously (Sonobe et al 2005). In brief, after centrifugation of sorted cell suspensions, total RNA was extracted from the pellet using RNeasy Mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol.…”

“…Real time PCR was performed using an ABI PRISM ® as described previously (Sonobe et al 2005). In brief, the primers were designed on different exons using Primer Express ® software version 2.0 (Applied Biosystems, Piscataway, NJ, USA) and each PCR product was confi rmed as a single band by agarose gel electrophoresis analysis.…”

Association of Interleukin-27 rs 153109 Single Nucleotide Polymorphism with Spontaneous Resolution of Hepatitis C Virus - Genotype 4a Infection in Egyptian Patients

“…Upon binding to its receptor in naїve CD4+ T cells, IL-27 activates JAK/STAT signaling inducing transcription of effector genes (Ihle, 2001;Takeda et al, 2003;Sonobe et al, 2005). IL-27 was reported previously to exert both pro-inflammatory and anti-inflammatory effects and have a role in acute phase response to infections (Ihle 2001;Pflanz et al, 2002;Takeda et al, 2003;Sonobe et al, 2005).…”

“…It has a pivotal effect on T-cells and acts on innate immune cells (Pflanz et al, 2002).IL-27 is comprised of two subunits, IL-27 p28 and EBI3 (Sprecher, Grant et al, 1998;Morishima, Mizoguchi et al, 2010).IL-27 binds to receptor complex composed of IL-27Rα (also called WSX-1) and gp 130 (Pflanz et al, 2004;Kastelein et al, 2007). Upon binding to its receptor in naїve CD4+ T cells, IL-27 activates JAK/STAT signaling inducing transcription of effector genes (Ihle, 2001;Takeda et al, 2003;Sonobe et al, 2005). IL-27 was reported previously to exert both pro-inflammatory and anti-inflammatory effects and have a role in acute phase response to infections (Ihle 2001;Pflanz et al, 2002;Takeda et al, 2003;Sonobe et al, 2005).…”