Production of hydroxy marilone C as a bioactive compound from Streptomyces badius

Abstract

Hydroxy marilone C is a bioactive metabolite produced from the culture broth of Streptomyces badius isolated from Egyptian soil. Hydroxy marilone C was purified and fractionated by a silica gel column with a gradient mobile phase dichloromethane (DCM):methanol then Sephadex LH-20 column using methanol as a mobile phase. It was subjected to many procedures such as infrared (IR), nuclear magnetic resonance (NMR), Mass spectroscopy (MS) and UV spectroscopy for elucidation of its structure. It was evaluated for antioxidant, cytotoxicity against human alveolar basal epithelial cell line (A-549) and human breast adenocarcinoma cell line (MCF-7) and antiviral activities; showed that the maximum antioxidant activity was 78.8% at 3 mg/ml after 90 min. and the IC50 value against DPPH radical found about 1.5 mg/ml after 60 min. Using MTT assay the effect of the pure compound on the proliferation of A-549 cells and MCF-7 cells was 443 μg/ml and 147.9 μg/ml, respectively, while for detection of antiviral activity using Madin–Darby canine kidney (MDCK) cells the maximum cytotoxicity was at 27.9% and IC50 was 128.1 μg/ml. The maximum concentration required for protecting 50% of the virus-infected cells against H1N1 viral cytopathogenicity (EC50) was 33.25% for 80 μg/ml. These results indicated that the hydroxy marilone C has potential antitumor and antiviral activities.

Antiviral, Cytotoxic, and Antioxidant Activities of Three Edible Agaricomycetes Mushrooms: Pleurotus columbinus, Pleurotus sajor-caju, and Agaricus bisporus

“…Cytotoxicity was assessed using MTT assay [ 37 ]. Aliquots (100 µL) of cell suspensions (cell density 1.2–1.8 × 10 4 cells/well) were placed in 96-well plates, complete medium (2.5) was added, and cells were incubated for 24 h. Then cells were treated with serial dilutions of fungal extracts for 48 h. MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide, 5 mg/mL in PBS) was added to wells and incubated for 4 h. Formazan crystals were extracted using MTT solubilization solution (10% Triton X-100 plus, 0.1 M HCl in anhydrous isopropanol) and at 570 nm absorbance were read using microplate reader.…”

Oligomycin-producing Streptomyces sp. newly isolated from Swiss soils efficiently protect Arabidopsis thaliana against Botrytis cinerea

“…For instance, dinactin has shown antagonistic activity against the rubber anthracnose fungus Colletotrichum gloeosporioides by inhibiting the production and germination of its spores ( 39 ). Additionally, hydroxy marilone C, produced by S. badius , displayed potent antioxidant, antitumor, and antiviral activities ( 40 ). Another strain of S. badius isolated from a Sahara soil exhibited considerable antagonistic activities against Fusarium culmorum , Verticillium dahliae, and Fusarium oxysporum f. sp.…”

Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms

“…Cytotoxicity was assessed using the MTT assay [ 109 ]. Aliquots (100 µL) of cell suspensions (cell density 1.2–1.8 × 10 4 cells/well) were placed in 96-well plates, complete medium was added and cells were incubated for 24 h. Then, cells were treated with serial dilutions of mushroom extracts for 48 h. MTT solution (5 mg/mL) in PBS was added to wells and incubated for 4 h, and absorbance at 570 nm was read using a microplate reader.…”