Production of Hydrogen Sulfide by Streptomycetes and Methods for its Detection

Küster, Eberhard; Williams, Stanley T.

doi:10.1128/aem.12.1.46-52.1964

Cited by 66 publications

(16 citation statements)

References 16 publications

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“…The new biofilm produces, among other metabolites, hydrogen sulfide (H 2 S), suggested to be part of the pathogenesis of periodontal disease because it is well known for its toxicity. H 2 S is produced by bacterial degradation of sulfur‐containing amino acids, for example, cysteine (Kuester & Williams, ) by many different bacterial strains (Basic, Blomqvist, Carlén, & Dahlén, ; Persson, Claesson, & Carlsson, ), and its presence has been reported in gingival crevicular fluid (Persson, ).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen sulfide exposure induces NLRP3 inflammasome‐dependent IL‐1β and IL‐18 secretion in human mononuclear leukocytes in vitro

Basic

Alizadehgharib

Dahlén

et al. 2017

Clinical & Exp Dental Res

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The aim was to investigate if hydrogen sulfide (H2S) induces the formation of the NLRP3 inflammasome and subsequent IL‐1β and IL‐18 secretion in human peripheral blood mononuclear cells (PBMCs) and in the human monocyte cell line THP1. Bacterial production of H2S has been suggested to participate in the inflammatory host response in periodontitis pathogenesis. H2S is a toxic gas with pro‐inflammatory properties. It is produced by bacterial degradation of sulfur‐containing amino acids, for example, cysteine. We hypothesize that H2S affects the inflammatory host response by inducing formation of the NLRP3 inflammasome and thereby causes the secretion of IL‐1ß and IL‐18. PBMCs from eight healthy blood donors, the human monocyte cell line THP1 Null, and two variants of the THP1 cell line unable to form the NLRP3 inflammasome were cultured in the presence or absence of 1 mM sodium hydrosulfide (NaHS) in 24‐well plates at 37°C for 24 hr. Supernatants were collected and the IL‐1β and IL‐18 concentrations were measured with DuoSet ELISA Development kit. PBMCs exposed to NaHS produced more IL‐1ß and IL‐18 than unexposed control cells (p = .023 and p = .008, respectively). An increase of extracellular potassium ions (K+) inhibited the secretion of IL‐1ß and IL‐18 (p = .008). Further, NaHS triggered the secretion of IL‐1ß and IL‐18 in human THP1‐Null monocytes (p = .0006 and p = .002, respectively), while the NaHS‐dependent secretion was reduced in the monocyte cell lines unable to form the NLRP3 inflammasome. Hence, the results suggest that NaHS induces the formation of the NLRP3 inflammasome and thus the secretion of IL‐1ß and IL‐18. Enhanced NLRP3 inflammasome‐dependent secretion of IL‐1β and IL‐18 in human mononuclear leukocytes exposed to NaHS in vitro is reported. This may be a mode for H2S to contribute to the inflammatory host response and pathogenesis of periodontal disease.

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Section: Introductionmentioning

confidence: 99%

Hydrogen sulfide exposure induces NLRP3 inflammasome‐dependent IL‐1β and IL‐18 secretion in human mononuclear leukocytes in vitro

Basic

Alizadehgharib

Dahlén

et al. 2017

Clinical & Exp Dental Res

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“…Utilization of different nitrogen sources, catalase production and degradation of tyrosine, hypoxanthine, casein, starch and gelatin were detected in modified Bennett's agar medium (MBA) after 7, 14 and 21 days, as described by Williams et al (1983). Hydrogen sulphide production was detected by the method of Küster & Williams (1964). The effect of different temperatures and pH levels on growth and tolerance to salt (NaCl at 5, 10 and 15 %, w/v) was determined by using MBA or medium A as a basal medium.…”

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confidence: 99%

Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt

Hozzein

Ali

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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An alkaliphilic actinomycete strain, designated YIM 80379T, was isolated from a soil sample collected from the eastern desert of Egypt and subjected to polyphasic taxonomy. The strain produced substrate and aerial mycelia on different media, with an optimum pH for growth of 9·5–10 and scarce or no growth at pH 7. Strain YIM 80379T contained meso-diaminopimelic acid, no diagnostic sugars, type PIII phospholipids and MK-10(H6) and MK-10(H8) as the predominant menaquinones. All of these characters assign isolate YIM 80379T consistently to the genus Nocardiopsis. This was confirmed by 16S rDNA analysis. It can be differentiated from all Nocardiopsis species with validly published names by phenotypic characteristics, phylogenetic analysis and DNA–DNA hybridization results. On the basis of polyphasic evidence, a novel species, Nocardiopsis alkaliphila sp. nov., is proposed. The type strain of the species is YIM 80379T (=CCTCC AA001031T=DSM 44657T).

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“…Acid production from carbohydrates, nitrogen sources utilization and substrate degradation were evaluated using the media described by Gordon et al [31] and Williams et al [32]. Nitrate reduction and H 2 S production were also studied following standard methods [33, 34]. Enzyme activities were determined using the API ZYM system (bioMérieux) following the instructions of manufacturer.…”

Section: Cultural Morphological and Physiological Propertiesmentioning

confidence: 99%

Pseudonocardia acidicola sp. nov., a novel actinomycete isolated from peat swamp forest soil

Klaysubun

Lipun

Duangmal

2020

International Journal of Systematic and Evolutionary Microbiology

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A novel actinobacterium, designated strain K10HN5T, was isolated from a peat soil sample collected from Kantulee peat swamp forest, Surat Thani Province, Thailand and its taxonomic position was determined using a polyphasic approach. Strain K10HN5T contained meso-diaminopimelic acid, arabinose, galactose, glucose and ribose in its whole-cell hydrolysates. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C16 : 0, iso-C15 : 0 and iso-C16 : 1H. Mycolic acids were not present. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylmethylethanolamine, hydroxyphosphatidylethanolamine, hydroxyphosphatidylmethylethanolamine and phosphatidylinositol. The 16S rRNA gene sequence analysis indicated that it was closely related to Pseudonocardia bannensis DSM 45300T (97.9 %) and Pseudonocardia xinjiangensis JCM 11839T (97.9 %). Strain K10HN5T exhibited low average nucleotide identity and digital DNA–DNA hybridization values with P. bannensis DSM 45300T (82.6, 28.7 %) and P. xinjiangensis JCM11839T (76.3, 22.2 %). The DNA G+C content of strain K10HN5T was 72.4 mol%. Based on polyphasic data, strain K10HN5T represents a novel species of the genus Pseudonocardia , for which the name Pseudonocardia acidicola sp. nov. is proposed. The type strain is K10HN5T (=TBRC 10048T=NBRC 113897T).

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Production of Hydrogen Sulfide by Streptomycetes and Methods for its Detection

Cited by 66 publications

References 16 publications

Hydrogen sulfide exposure induces NLRP3 inflammasome‐dependent IL‐1β and IL‐18 secretion in human mononuclear leukocytes in vitro

Hydrogen sulfide exposure induces NLRP3 inflammasome‐dependent IL‐1β and IL‐18 secretion in human mononuclear leukocytes in vitro

Nocardiopsis alkaliphila sp. nov., a novel alkaliphilic actinomycete isolated from desert soil in Egypt

Pseudonocardia acidicola sp. nov., a novel actinomycete isolated from peat swamp forest soil

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