Production of Hydrogen Peroxide by Serum and its Involvement in Cell Proliferation in ROS 17/2.8 Osteoblasts

Abstract

The effects of hydrogen peroxide, which fetal bovine serum (FBS) releases, on proliferation have been studied in ROS 17/2.8 osteoblasts. Cell proliferation, when activated by FBS, was inhibited by catalase in ROS 17/2.8 osteoblasts, but did not in primary osteoblast-like cells. Serum-induced extracellular signal-regulated kinase(ERK) activity was reduced by the pretreated catalase in ROS 17/2.8 osteoblasts. In addition, the present studies demonstrate that addition of FBS led to an increase of fluorescence of dihydrorhodamine 123, indicating formation of free radicals including hydrogen peroxide in ROS 17/2.8 osteoblasts, but not in primary osteoblast-like cells. These phenomena may account for the generation of reactive oxygen species during cellular proliferation in ROS 17/2.8 osteoblasts.

Biological effects of a complex of vanadium(V) with salicylaldehyde semicarbazone in osteoblasts in culture: Mechanism of action

“…Intracellular H 2 O 2 was measured spectrofotometrically, using the leuco probe DHR 123 which preferentially reacts with H 2 O 2 , and is oxidized to the fluorescent molecule rhodamine 123 [30,31]. Briefly, the osteoblast cell lines were incubated with V(V)-Salsem for 24 h followed by an incubation with DHR for 30 min at 37°C.…”

Linking In Vitro Models of Endothelial Dysfunction with Cell Senescence

“…The effects of H 2 O 2 on HUVECs could be explained, partially, by the increase in expression of P21 because of its association with cell cycle arrest and induction of cell senescence [12]. Differences in results in the current literature despite using identical concentrations of H 2 O 2 , and similar results but at different concentrations of H 2 O 2 may be due to differences in cell passage, medium used, the presence of confounding antioxidants, such as phenol red, or the concentration of FBS [32,33].…”

Knockouts of Se‐glutathione peroxidase‐1 and Cu,Zn superoxide dismutase exert different impacts on femoral mechanical performance of growing mice

“…While H 2 O 2 was generated during osteoblast proliferation [43] and stimulated osteoclastic resorption in vitro [9], osteoclast formation was abrogated in RAW cells overexpressing GPX1 [15]. Thus, elevated intracellular H 2 O 2 tone in GPX1( -/ -) mice augmented both bone formation and resorption, leading to increased bone turnover, but no net change in the overall bone mechanical properties.…”