Production of Hyaluronidase and Beta-Glucuronidase by Viridans Streptococci Isolated from Gingival Crevices

Schultz-Haudt, Stig D.; Scherp, Henry W.

doi:10.1177/00220345550340061901

Cited by 59 publications

(12 citation statements)

References 7 publications

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“…In the present study the appearance of /]-glucuronidase activity in the keratinized surface layers of the hyperplastic and inflamed gingiva was thought to originate from micro-organisms. This suggestion is supported by the previous investigations, in which it has been demonstrated that the number of Strepto-coccus viridans, known to be /i-glucuronidase producers increases during gingivitis (Schultz-Haudt & Scherp 1955) and that the penetration of this enzyme into the keratinized layer of the epithelium might be possible, although no penetration through the entire thickness of stratum corneum into the underlying layers of the epithelium occurs (McDougall 1971), The suggestion of the penetration of jjglucuronidase into the keratinized layer reported in the present study was thought to be due to the hypothesis that even the keratinized surface layer of stratum corneum in the hydantoin induced hyperplastic as well as inflamed gingiva are injured and thus certain enzymes and toxins might diffundate through it.…”

Section: Discussionsupporting

confidence: 83%

A histochemical study of glycosidases in hydantoin induced hyperplastic, healthy and inflamed human gingiva

Larmas

1974

J of Periodontal Research

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Fresh samples of hydantoin induced hyperplastic, healthy and inflamed human gingiva were studied histochemically using various azo dyes for β‐glucuronidase (EC 3.2.1.31), for β‐ and β‐glucosidase (EC 3.2.1.20 and 3.2.1.21) as well as β‐galactosidase (EC 3.2.1.23) and for N‐acetylglucosaminidase (EC 3.2.1.30), in order to add support to the hypothesis that hydantoin induced hyperplasia is always connected with inflammation. Moderate β‐glucuronidase activity was observed in healthy, inflamed and hydantoinhyperplastic gingiva. The distribution of the enzyme activity was similar in all types of the tissue except the stratum corneum. The healthy gingiva did not reveal this activity whereas the inflamed and hydantoinhyperplastic gingiva did. The stratum basale and spinosum of the epithelium, the fibroblasts and the inflammatory cells, especially the macrophages, revealed enzyme activity in all types of tissues. In the healthy tissue only a few inflammatory cells were seen and thus the β‐glucuronidase activity was low when compared to inflamed or hyperplastic gingiva. Weak β‐galactosidase, N‐asetylglucosaminidase and β‐glucosidase activity was seen in all types of gingival samples. Enzyme activity was observed in the same structures as β‐glucuronidase with the exception of the stratum corneum, which revealed no activity. The relatively strong β‐glucuronidase activity in the keratinized cell layer of the epithelium of inflamed and hydantoinhyperplastic tissue may be due to the microbial enzyme diffusion into the keratinized cell layer of the injured tissue.

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Section: Discussionsupporting

confidence: 83%

A histochemical study of glycosidases in hydantoin induced hyperplastic, healthy and inflamed human gingiva

Larmas

1974

J of Periodontal Research

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“…In addition G U D activity has been detected in selected strains of several Gram-positive bacteria including Staphylococcus (Barber et al 1948;Sing and Ng 1986), Streptococcus (Robinson et al 1952;Jacox 1953;Williams 1954;Schultz-Haudt and Scherp 1955;Rod ef al. 1974), anaerobic corynebacteria (DahlCtn and Linde 1973) and Clostridium (Sakaguchi and Murata 1983).…”

Section: Introductionmentioning

confidence: 99%

Methods for Escherichia coli identification in food, water and clinical samples based on beta‐glucuronidase detection

Frampton

Restaino

1993

Journal of Applied Bacteriology

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“…Some glucose may also derive from break-down products of high molecular components of the gingival tissues and cells. The liberation and activation of hydrolyzing enzymes (Schultz-Haudt and Scherp 1955, 1956, Dewar 1958, Hampp, Mergenhagen and Omata 1959, Gibbons and MacDonald 1961 during the inflammatory process are likely to cause splitting of the gingival connective tissue intercellular matrix, which would result in the release of hexose and other small molecular substances like hexoronic acid and hexosamine. A similar enzyme activity may cause degradation of the polysaccharides found in the walls of the microorganisms in the area (Salton 1961).…”

Section: Discussionmentioning

confidence: 99%

Carbohydrate components of the gingival exudate

Hara¹,

Löe²

1969

J of Periodontal Research

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This paper describes the presence and amounts of free glucose, hexosamine and hexuronic acid in the gingival exudate. Exudate was sampled on filterstrips from individuals with different degrees of gingival inflammation. The strips were weighed before and after absorption of exudate and the exudate was calculated. The exudate was extracted and the content of glucose, hexosamine and hexuronic acid was determined colorimetrically. In keeping with previous studies the amount of exudate increased with the severity of inflammation. Exudate glucose varied between 1.13 and 7.09 μg/mg exudate. The hexosamine content was approximately 0.6 μg/mg and the hexuronic acid concentration varied between 50 and 60 μg/mg. The possible sources of origin of the carbohydrate components are discussed.

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Production of Hyaluronidase and Beta-Glucuronidase by Viridans Streptococci Isolated from Gingival Crevices

Cited by 59 publications

References 7 publications

A histochemical study of glycosidases in hydantoin induced hyperplastic, healthy and inflamed human gingiva

A histochemical study of glycosidases in hydantoin induced hyperplastic, healthy and inflamed human gingiva

Methods for Escherichia coli identification in food, water and clinical samples based on beta‐glucuronidase detection

Carbohydrate components of the gingival exudate

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