Production of hyaluronic acid from Streptococcus zooepidemicus MTCC 3523 and its wound healing activity

Patil, Kanchankumar P.; Patil, Deepak; Chaudhari, Bhushan L.; Chincholkar, S. B.

doi:10.1016/j.jbiosc.2010.10.012

Cited by 16 publications

(4 citation statements)

References 15 publications

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“…HA, either animal- or microbial-derived, must undergo meticulous purification processes to obtain a highly pure product that meets the specifications for clinical and cosmetic applications. For microbial HA, the downstream process often involves a combination of alcohol precipitation, adsorption on silica gel, and/or activated charcoal and diafiltration steps (Patil et al 2011 ; Rangaswamy and Jain 2008 ). Proteins are the main impurities present in microbial HA (Cavalcanti et al 2020 ), but endotoxins from pathogenic bacteria, such as S. zooepidemicus , also represent potential safety concerns (Liu et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

et al. 2021

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Given its biocompatibility, rheological, and physiological properties, hyaluronic acid (HA) has become a biomaterial of increasing interest with multiple applications in medicine and cosmetics. In recent decades, microbial fermentations have become an important source for the industrial production of HA. However, due to its final applications, microbial HA must undergo critical and long purification processes to ensure clinical and cosmetic grade purity. Aqueous two-phase systems (ATPS) have proven to be an efficient technique for the primary recovery of high-value biomolecules. Nevertheless, their implementation in HA downstream processing has been practically unexplored. In this work, polyethylene glycol (PEG)–citrate ATPS were used for the first time for the primary recovery of HA produced with an engineered strain of Streptococcus equi subsp. zooepidemicus. The effects of PEG molecular weight (MW), tie-line length (TLL), volume ratio (VR), and sample load on HA recovery and purity were studied with a clarified fermentation broth as feed material. HA was recovered in the salt-rich bottom phase, and its recovery increased when a PEG MW of 8000 g mol−1 was used. Lower VR values (0.38) favoured HA recovery, whereas purity was enhanced by a high VR (3.50). Meanwhile, sample load had a negative impact on both recovery and purity. The ATPS with the best performance was PEG 8000 g mol−1, TLL 43% (w/w), and VR 3.50, showing 79.4% HA recovery and 74.5% purity. This study demonstrated for the first time the potential of PEG–citrate ATPS as an effective primary recovery strategy for the downstream process of microbial HA.

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Section: Introductionmentioning

confidence: 99%

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

et al. 2021

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“…Na área clínica pode atuar como protetor, repositor e potenciador de tecidos, além de proteger tecidos da dessecação e de agentes nocivos (BALAZS, 2004). A produção de ácido hialurônico por Streptococcus é bastante conhecida, estando presente nas cápsulas das espécies Streptococcus thermophilus (IZAWA et al, 2009), Streptococcus pyogenes (KANG et al, 2012) e Streptococcus zooepidemicus (PATIL et al, 2011), além de Chlorella e Pasteurella multocida (DeANGELIS, 2012). Entretanto, a inserção de genes has que codificam para a enzima hialurona sintase, responsável por sua síntese, também foi eficiente para Bacillus subtilis (WIDNER et al, 2005) e Lactococcus lactis (CHIEN;LEE, 2007) produzirem esse polímero.…”

Section: Seleção De Bactérias Com Características De Rpcpunclassified

Bactérias associadas às cactáceas da Caatinga: promoção de crescimento de plantas sob estresse hídrico

Kavamura¹

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AGRADECIMENTOS Silva, Cassiano Forner, José Abrahão Haddad Galvão, Leonardo José da Silva (Léo), Lúcio Bertoldo Costa, Michelli de Souza dos Santos, Milena Duarte Lançoni, Rafael Eduardo Silva, Regiane Iost (Figura) A Caatinga, bioma exclusivamente brasileiro, inserido no clima semiárido nordestino, apresenta xerófitas com alta resistência aos períodos de seca. Estas plantas associam-se a micro-organismos que também se encontram bem adaptados, desenvolvendo mecanismos de proteção celular contra o estresse hídrico, assim como proteção vegetal contra os efeitos negativos da dessecação. O presente estudo buscou compreender as bactérias associadas às cactáceas da Caatinga, analisando a estrutura das comunidades bacterianas de solo e da rizosfera de Cereus jamacaru durante a alteração do período chuvoso para o de seca, identificando os grupos dominantes e discutindo algumas funções que possibilitem a manutenção da interação solo-cacto-micro-organismo durante o período de seca. Além disso, buscou selecionar bactérias tolerantes à seca e que fossem capazes de promover crescimento de plantas sob estresse hídrico. Amostras foram coletadas ao longo da Caatinga, em cinco estados: BA, CE, PI, PB e RN totalizando cinco pontos. Com o uso de metodologias independentes de cultivo, foi possível observar que o período de amostragem (chuvoso/seca) foi o principal responsável pela alteração na estrutura das comunidades bacterianas. Os filos Proteobacteria e Bacteroidetes foram abundantes durante o período chuvoso e os filos Actinobacteria e o gênero Bacillus abundantes durante o período de seca. Com o uso de metodologias dependentes de cultivo, foram isoladas com bastante frequência linhagens pertencentes ao gênero Bacillus, capazes de crescer em meio com reduzida atividade de água e com alguns mecanismos de proteção contra a dessecação, como a produção de exopolissacarídeos e biofilme. Além disso, várias linhagens apresentaram mecanismos de promoção de crescimento de plantas diretos e/ou indiretos, como produção de fitohormônio, disponibilização de P por meio de solubilização, fixação de nitrogênio, redução dos efeitos negativos do estresse causados por etileno, produção de celulase e amônia. Uma linhagem de Bacillus sp. foi capaz de promover crescimento de milho sob estresse hídrico, incrementando alguns parâmetros vegetais analisados. São discutidos o uso de consórcio bacteriano entre duas linhagens, além dos mecanismos que os micro-organismos dispõem para tolerar condições ambientais adversas e ainda as funções que estes micro-organismos podem ter na proteção vegetal contra a dessecação. Caatinga, a Brazilian unique biome, inserted in the semi-arid climate of Brazilian northeast, presents xerophytes with high resistance to drought periods. These plants are found in association with well-adapted microorganisms that have evolved some cellular protection mechanisms against water stress, as well as plant protection against the negative effects of desiccation. This work aimed to study the bacteria associated to some Caatinga cact...

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“…ID9102 [13] , S. zooepidemicus ATCC 35426 [14] , S. zooepidemicus WSH-24 [15] , S zooepidemicus H23 [16] , S. zooepidemicus NJUST01 [17] , S. equi. ss equi CCUG 22971 (Stahl, S., US patent 2003-0134393 A1, 2003), S. zooepidemicus #104 (Shiseido, Yokohama) [18] , S. zooepidemicus ATCC 35246 HA+ Lac+ Ems (1), S. equi Ferm BP-879 and S. zooepidemicus BP-878 (Morita, H., and Fuji, M., US patent 5071751, 1991), S. equi FM 100 (Hashimoto, M., Saegusa, H., Chiba, S., Kitagawa, H., Miyoshi, T., US patent 4946780, 1990) and S. zooepidemicus MTCC 3523 [19] have been subjected to HA production studies, exploring novel strains with intrinsic ability of high HA production remains highly valuable to meet the growing HA demand. In the present study, we exerted a combinatorial strategy to achieve the high quality and quantity HA.…”

Section: Introductionmentioning

confidence: 99%

Enhanced hyluronic acid production in Streptococcus zooepidemicus by over expressing HasA and molecular weight control with Niscin and glucose

Zakeri

Rasaee

Pourzardosht

2017

Biotechnology Reports

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HighlightsPerforming these improvement on a wild type Streptococcus zooepidemicus which is already capable of high yield HA production would circumvent the common obstacles lies ahead of industrial level fermentative HA production. Having an improved strain and optimized culture media would pave the way for industrial grade HA production with high quality and quantity.HasA gene was amplified and introduced into the wild type Streptococcus zooepidemicus, under the control of Nisin promoter. The HasA over-expression increased the HA production. In order to compensate for molecular weight loss, the glucose concentration was increased to an optimum amount of 90 g/L.

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Production of hyaluronic acid from Streptococcus zooepidemicus MTCC 3523 and its wound healing activity

Cited by 16 publications

References 15 publications

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

Primary recovery of hyaluronic acid produced in Streptococcus equi subsp. zooepidemicus using PEG–citrate aqueous two-phase systems

Bactérias associadas às cactáceas da Caatinga: promoção de crescimento de plantas sob estresse hídrico

Enhanced hyluronic acid production in Streptococcus zooepidemicus by over expressing HasA and molecular weight control with Niscin and glucose

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