Production of human recombinant phenylalanine hydroxylase in Lactobacillus plantarum for gastrointestinal delivery

Ramirez, Aura M; Rodríguez-López, Alexander; Ardila, Andrea; Beltran, Laura; Patarroyo, Camilo; Melendez, Adelina del Pilar; Sánchez, Oscar F.; Alméciga-Díaz, Carlos J.

doi:10.1016/j.ejps.2017.07.033

Cited by 16 publications

(7 citation statements)

References 49 publications

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“…paracasei expressing angiotensin-(1-7). Oral administration of the strain reduced the loss of retinal vascular capillaries, ganglion cells, and decreased the expression of inflammatory cytokines [157].…”

Section: Recombinant Probiotics For the Treatment Of Metabolic Disordersmentioning

confidence: 95%

Recombinant Probiotic Preparations: Current State, Development and Application Prospects

Khablenko

Danylenko

Yalovenko

et al. 2023

Innov Biosyst Bioeng

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The article is devoted to the latest achievements in the field of research, development, and implementation of various types of medicinal products based on recombinant probiotics. The benefits of probiotics, their modern use in medicine along with the most frequently used genera and species of probiotic microorganisms were highlighted. The medicinal and therapeutic activities of the studied probiotics were indicated. The review suggests various methods of creating recombinant probiotic microorganisms, including standard genetic engineering methods, as well as systems biology approaches and new methods of using the CRISPR-Cas system. The range of potential therapeutic applications of drugs based on recombinant probiotics was proposed. Special attention was paid to modern research on the creation of new, more effective recombinant probiotics that can be used for various therapeutic purposes. Considering the vast diversity of therapeutic applications of recombinant probiotics and ambiguous functions, their use for the potential treatment of various common human diseases (non-infectious and infectious diseases of the gastrointestinal tract, metabolic disorders, and allergic conditions) was investigated. The prospects for creating different types of vaccines based on recombinant probiotics together with the prospects for their implementation into medicine were considered. The possibilities of using recombinant probiotics in veterinary medicine, particularly for the prevention of domestic animal diseases, were reviewed. The prospects for the implementation of recombinant probiotics as vaccines and diagnostic tools for testing certain diseases as well as modeling the work of the human digestive system were highlighted. The risks of creation, application, including the issues related to the regulatory sphere regarding the use of new recombinant microorganisms, which can potentially enter the environment and cause unforeseen circumstances, were outlined.

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Section: Recombinant Probiotics For the Treatment Of Metabolic Disordersmentioning

confidence: 95%

Recombinant Probiotic Preparations: Current State, Development and Application Prospects

Khablenko

Danylenko

Yalovenko

et al. 2023

Innov Biosyst Bioeng

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show abstract

“…The probiotic properties of traditional probiotics, such as EcN, Bifidobacterium species, − and Lactobacillus species, ,,− have been extensively described. Traditional probiotics can protect the intestinal mucosa, − modulate gut microbiota, and produce beneficial metabolites .…”

Section: Chassismentioning

confidence: 99%

Intestinal Engineered Probiotics as Living Therapeutics: Chassis Selection, Colonization Enhancement, Gene Circuit Design, and Biocontainment

Huang

Lin

et al. 2022

ACS Synth. Biol.

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Intestinal probiotics are often used for the in situ treatment of diseases, such as metabolic disorders, tumors, and chronic inflammatory infections. Recently, there has been an increased emphasis on intelligent, customized treatments with a focus on long-term efficacy; however, traditional probiotic therapy has not kept up with this trend. The use of synthetic biology to construct gut-engineered probiotics as live therapeutics is a promising avenue in the treatment of specific diseases, such as phenylketonuria and inflammatory bowel disease. These studies generally involve a series of fundamental design issues: choosing an engineered chassis, improving the colonization ability of engineered probiotics, designing functional gene circuits, and ensuring the safety of engineered probiotics. In this review, we summarize the relevant past research, the progress of current research, and discuss the key issues that restrict the widespread application of intestinal engineered probiotic living therapeutics.

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“…Examining microbiome dynamics during colonization (whether is it permanent or not) or whether the microbes were prone to adapt (mutate) within the gut-environment is important to determine safety of a given probiotic ( 47 ). In PKU, recent scientific advances give rise to the use of genetically modified probiotics to lower the absorption of Phe from the gut by relying on microbial metabolism ( 47 – 50 ). However, although they have shown to be stable as probiotics, little is known about how these supplements affect the ecological and evolutionary dynamics of commensal microbiota.…”

Section: Eco-evolutionary Aspects Associated With Probiotic Supplemenmentioning

confidence: 99%

A Microbial Community Ecology Perspective on the Gut-Microbiome-Brain Axis

et al. 2020

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Production of human recombinant phenylalanine hydroxylase in Lactobacillus plantarum for gastrointestinal delivery

Cited by 16 publications

References 49 publications

Recombinant Probiotic Preparations: Current State, Development and Application Prospects

Recombinant Probiotic Preparations: Current State, Development and Application Prospects

Intestinal Engineered Probiotics as Living Therapeutics: Chassis Selection, Colonization Enhancement, Gene Circuit Design, and Biocontainment

A Microbial Community Ecology Perspective on the Gut-Microbiome-Brain Axis

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