Glycosylation 2012
DOI: 10.5772/51301
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Production of Highly Sialylated Monoclonal Antibodies

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Cited by 13 publications
(33 citation statements)
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“…2B). 47 The DNA mix composition selected was thus 2% of GT plasmid and 20% of ST6 plasmid ( Fig. 2A-B).…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…2B). 47 The DNA mix composition selected was thus 2% of GT plasmid and 20% of ST6 plasmid ( Fig. 2A-B).…”
Section: Resultsmentioning
confidence: 99%
“…A HC:LC ratio of 2:3 (w:w) was used to ensure optimal expression of the mAb in CHO-3E7 cells as determined in a previous work. 47 The human membrane-associated b-1,4-galactosyltransferase 1 (GT) and b-galactoside a-2,6-sialyltransferase 1 (ST6) were co-expressed with the mAbs to enhance galactosylation or a2,6-sialylation of the Fc. The quantity of GT-encoding plasmid necessary to achieve maximal galactosylation of the Fc was first assessed.…”
Section: Resultsmentioning
confidence: 99%
“…Much work has been devoted to humanizing sialylation with the introduction of the ST6 gene but limited increase in 2,6-linked sialic acid, has been observed in the recombinant protein (El Mai et al 2013). It is thought that increasing the 2,6 sialylation of Mabs has been difficult, due to accessibility of the ST6 to the glycan (Raymond et al 2012). However, this can be overcome by the replacement of a phenylalanine residue with alanine in the Fc region of the Mab (Jassal et al 2001;Raymond et al 2012).…”
Section: Glycosylation Engineering and Modification Of Glycan Structurementioning
confidence: 99%
“…It is thought that increasing the 2,6 sialylation of Mabs has been difficult, due to accessibility of the ST6 to the glycan (Raymond et al 2012). However, this can be overcome by the replacement of a phenylalanine residue with alanine in the Fc region of the Mab (Jassal et al 2001;Raymond et al 2012). In another report a mutation of Y407E (tyrosine to glutamate) residue in the CH3 region of IgG1 results in increased sialylation and galactosylation possibly through allosteric affects on the CH2 region and CH2-CH3 interface (Rose et al 2013).…”
Section: Glycosylation Engineering and Modification Of Glycan Structurementioning
confidence: 99%
“…Glycation, at lysine residues, appeared to be mainly on the surface of the BSA carrier. (Raju et al, 2011) Production of highly sialylated monoclonal antibodies 48 (Raymond et al, 2012) Role of glycosylation in therapeutic antibodies 213 (Spearman et al, 2011) Addition of polysialic acid chains to biantennary glycans to modify half-life Anti-HER2/neu receptor IgG1 Pichia pastoris TOF, glycans Development of a method to purify the product. Endogenous glycans…”
Section: B Synthesis and Analysis Of Carbohydrate-protein Conjugatesmentioning
confidence: 99%