Production of highly concentrated and hyperpolarized metabolites within seconds in high and low magnetic fields

Korchak, Sergey; Emondts, Meike; Mamone, Salvatore; Blümich, Bernhard

doi:10.1039/c9cp05227e

Cited by 34 publications

(103 citation statements)

References 78 publications

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“…Experimentally, this is manifested in an asymmetric broadening of spectral lines (Fig, S10, SI) and RASER bursts, which may confound the interpretation of the spectra or cause inefficient polarization transfer to heteronuclei. The effect of RD on 1 H-PASADENA spectra and on the efficiency of polarization transfer to 13 C was recently and experimentally demonstrated by Korchak et al [35] at room temperature at fields of 1 T and 7 T.…”

Section: Figure 2 Spontaneous 1 H Phip-raser At 600mentioning

confidence: 81%

“…Simulations were performed using the MOIN spin library; the source code is available online. [33,34] The addition of RD into the LvN equation makes the simulation of polarization transfer in the presence of RD [35] straightforward. Using the published SABRE models including chemical exchange, the same approach can be used to simulate SABRE-RASER.…”

Section: Figure 2 Spontaneous 1 H Phip-raser At 600mentioning

confidence: 99%

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Continuous Radio Amplification by Stimulated Emission of Radiation using Parahydrogen Induced Polarization (PHIP‐RASER) at 14 Tesla

2020

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Nuclear Magnetic Resonance (NMR) is an intriguing quantum‐mechanical effect that is used for routine medical diagnostics and chemical analysis alike. Numerous advancements have contributed to the success of the technique, including hyperpolarized contrast agents that enable real‐time imaging of metabolism in vivo. Herein, we report the finding of an NMR radio amplification by stimulated emission of radiation (RASER), which continuously emits 1H NMR signal for more than 10 min. Using parahydrogen induced hyperpolarization (PHIP) with 50 % para‐hydrogen, we demonstrated the effect at 600 MHz but expect that it is functional across a wide range of frequencies, e.g. 101–103 MHz. PHIP‐RASER occurs spontaneously or can be triggered with a standard NMR excitation. Full chemical shift resolution was maintained, and a linewidth of 0.6 ppb was achieved. The effect was reproduced by simulations using a weakly coupled, two spin‐1/2 system. All devices used were standard issue, such that the effect can be reproduced by any NMR lab worldwide with access to liquid nitrogen for producing parahydrogen.

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Section: Figure 2 Spontaneous 1 H Phip-raser At 600mentioning

confidence: 81%

Section: Figure 2 Spontaneous 1 H Phip-raser At 600mentioning

confidence: 99%

Continuous Radio Amplification by Stimulated Emission of Radiation using Parahydrogen Induced Polarization (PHIP‐RASER) at 14 Tesla

2020

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“…Research is on‐going to increase the sensitivity 10000‐fold or more through hyperpolarisation. This would accomplish a 13 C spectrum in one scan, 70–74 or enable metabolite analysis in sec 75 . SABRE (signal amplification by reversible exchange) is such a promising technique but it is not universal for all analytes, requires additional chemicals and logistically it is rather complicated 70–72 .…”

Section: Discussionmentioning

confidence: 99%

Low‐field benchtop NMR spectroscopy: status and prospects in natural product analysis^†

Beek

2020

Phytochemical Analysis

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Introduction Since a couple of years, low‐field (LF) nuclear magnetic resonance (NMR) spectrometers (40–100 MHz) have re‐entered the market. They are used for various purposes including analyses of natural products. Similar to high‐field instruments (300–1200 MHz), modern LF instruments can measure multiple nuclei and record two‐dimensional (2D) NMR spectra. Objective To review the commercial availability as well as applications, advantages, limitations, and prospects of LF‐NMR spectrometers for the purpose of natural products analysis. Method Commercial LF instruments were compared. A literature search was performed for articles using and discussing modern LF‐NMR. Next, the articles relevant to natural products were read and summarised. Results Seventy articles were reviewed. Most appeared in 2018 and 2019. Low costs and ease of operation are most often mentioned as reasons for using LF‐NMR. Conclusion As the spectral resolution of LF instruments is limited, they are not used for structure elucidation of new natural products but rather applied for quality control (QC), forensics, food and health research, process control and teaching. Chemometric data handling is valuable. LF‐NMR is a rapidly developing niche and new instruments keep being introduced.

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“…In a second step, the hyperpolarization can be transferred to other nuclei, e.g. 13 C or 15 N [25][26][27][28][29][30][31][32] . PHIP is renowned for being inexpensive, simple to use, and allows for the production of hyperpolarized substrates with a high repetition cycle.…”

Section: Introductionmentioning

confidence: 99%

Singlet-Contrast Magnetic Resonance Imaging

Eills

Cavallari²,

Kircher³

et al. 2020

Preprint

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Hyperpolarization-enhanced magnetic resonance imaging can be used to study biomolecular processes in the body, but typically requires nuclei such as 13C, 15N, or 129Xe due to their long spin‑polarization lifetimes and the absence of a proton‑background signal from water and fat in the images. Here we present a novel type of 1H imaging, in which hyperpolarized spin order is locked in a nonmagnetic long-lived correlated (singlet) state, and is only liberated for imaging by a specific biochemical reaction. In this work we produce hyperpolarized fumarate via chemical reaction of a precursor molecule with para-enriched hydrogen gas, and the proton singlet order in fumarate is released as antiphase NMR signals by enzymatic conversion to malate in D2O. Using this model system we show two pulse sequences to rephase the NMR signals for imaging and suppress the background signals from water. The hyperpolarization-enhanced 1H‑imaging modality presented here can allow for hyperpolarized imaging without the need for low‑abundance, low‑sensitivity heteronuclei.

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Production of highly concentrated and hyperpolarized metabolites within seconds in high and low magnetic fields

Abstract: We introduce two experiments that allow for the rapid production of hyperpolarized metabolites. More than 50% 13C polarization in 50 mM concentrations is achieved. This can be translated to portable low field NMR devices.

Cited by 34 publications

References 78 publications

Continuous Radio Amplification by Stimulated Emission of Radiation using Parahydrogen Induced Polarization (PHIP‐RASER) at 14 Tesla

Continuous Radio Amplification by Stimulated Emission of Radiation using Parahydrogen Induced Polarization (PHIP‐RASER) at 14 Tesla

Low‐field benchtop NMR spectroscopy: status and prospects in natural product analysis^†

Singlet-Contrast Magnetic Resonance Imaging

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Production of highly concentrated and hyperpolarized metabolites within seconds in high and low magnetic fields

Abstract: We introduce two experiments that allow for the rapid production of hyperpolarized metabolites. More than 50% 13C polarization in 50 mM concentrations is achieved. This can be translated to portable low field NMR devices.

Cited by 34 publications

References 78 publications

Continuous Radio Amplification by Stimulated Emission of Radiation using Parahydrogen Induced Polarization (PHIP‐RASER) at 14 Tesla

Continuous Radio Amplification by Stimulated Emission of Radiation using Parahydrogen Induced Polarization (PHIP‐RASER) at 14 Tesla

Low‐field benchtop NMR spectroscopy: status and prospects in natural product analysis†

Singlet-Contrast Magnetic Resonance Imaging

Contact Info

Product

Resources

About

Low‐field benchtop NMR spectroscopy: status and prospects in natural product analysis^†