Production of high-fructose syrup from date by-products in a packed bed bioreactor using a novel thermostable invertase from<i>Aspergillus awamori</i>

Smaali, Issam; Soussi, Asma; Bouallagui, Hassib; Chaira, Nizar; Hamdi, Moktar; Marzouki, M. Néjib

doi:10.3109/10242422.2011.615924

Cited by 17 publications

(8 citation statements)

References 28 publications

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“… 2 Normally, IFOSs are produced via enzymatic activity of β-fructofuranosidase (E.C. 3.2.1.26) derived from various fungal species such as Aspergillus niger , 3,4 Aspergillus japonicas , 5–7 Aspergillus aculeatus , 8,9 Aspergillus awamori , 10 Aspergillus kawachii , 11 and Aspergillus oryzae . 12 Nonetheless, the use of fungal enzymes had been shown to have some limitations because they prefer hydrolysis to transfructosylation, and most of the fungal enzymes specifically synthesize only a short chain of IFOS with a degree of polymerization (DP) in the range of 2–4.…”

Section: Introductionmentioning

confidence: 99%

“…Aspergillus aculeatus, 8,9 Aspergillus awamori, 10 Aspergillus kawachii, 11 and Aspergillus oryzae. 12 Nonetheless, the use of fungal enzymes had been shown to have some limitations because they prefer hydrolysis to transfructosylation, and most of the fungal enzymes specically synthesize only a short chain of IFOS with a degree of polymerization (DP) in the range of 2-4.…”

Section: -7mentioning

confidence: 99%

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Highly porous core–shell chitosan beads with superb immobilization efficiency forLactobacillus reuteri121 inulosucrase and production of inulin-type fructooligosaccharides

et al. 2018

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Section: Introductionmentioning

confidence: 99%

Section: -7mentioning

confidence: 99%

Highly porous core–shell chitosan beads with superb immobilization efficiency forLactobacillus reuteri121 inulosucrase and production of inulin-type fructooligosaccharides

et al. 2018

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“…The fungal strain A. awamori is of high industrial importance for bioprocesses; hence, it has been widely evaluated, particularly, in solid-state fermentation for the production of hydrolytic enzymes [11,21,22,23]. Smaali et al reported on the production of extracellular thermostable invertase during submerged cultures, triggered by the addition of sucrose (1%) [23]. Bertolin et al studied the production of glucoamylase via SSF of A .…”

Section: Resultsmentioning

confidence: 99%

Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams

Tsakona

Papadaki

Kopsahelis

et al. 2019

Foods

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Diversified mixed confectionery waste streams were utilized in a two-stage bioprocess to formulate a nutrient-rich fermentation media for microbial oil production. Solid-state fermentation was conducted for the production of crude enzyme consortia to be subsequently applied in hydrolytic reactions to break down starch, disaccharides, and proteins into monosaccharides, amino acids, and peptides. Crude hydrolysates were evaluated in bioconversion processes using the red yeast Rhodosporidium toruloides DSM 4444 both in batch and fed-batch mode. Under nitrogen-limiting conditions, during fed-batch cultures, the concentration of microbial lipids reached 16.6–17 g·L−1 with the intracellular content being more than 40% (w/w) in both hydrolysates applied. R. toruloides was able to metabolize mixed carbon sources without catabolite repression. The fatty acid profile of the produced lipids was altered based on the substrate employed in the bioconversion process. Microbial lipids were rich in polyunsaturated fatty acids, with oleic acid being the major fatty acid (61.7%, w/w). This study showed that mixed food side-streams could be valorized for the production of microbial oil with high unsaturation degree, pointing towards the potential to produce tailor-made lipids for specific food applications. Likewise, the proposed process conforms unequivocally to the principles of the circular economy, as the entire quantity of confectionery by-products are implemented to generate added-value compounds that will find applications in the same original industry, thus closing the loop.

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“…An effective immobilisation method of fructosyltransferases is highly desired as it would allow a continuous FOS production process, the reuse of the biocatalyst and the reduction of the total costs for industrial settings [25][26][27]. In addition, immobilisation often protects enzymes from inactivation at extreme pH, high temperatures or organic solvents [28;29].…”

mentioning

confidence: 99%

Dried alginate-entrapped enzymes (DALGEEs) and their application to the production of fructooligosaccharides

Fernández‐Arrojo

Rodríguez-Colinas

Gutiérrez-Alonso

et al. 2013

Process Biochemistry

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Production of high-fructose syrup from date by-products in a packed bed bioreactor using a novel thermostable invertase fromAspergillus awamori

Cited by 17 publications

References 28 publications

Highly porous core–shell chitosan beads with superb immobilization efficiency forLactobacillus reuteri121 inulosucrase and production of inulin-type fructooligosaccharides

Highly porous core–shell chitosan beads with superb immobilization efficiency forLactobacillus reuteri121 inulosucrase and production of inulin-type fructooligosaccharides

Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams

Dried alginate-entrapped enzymes (DALGEEs) and their application to the production of fructooligosaccharides

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