Production of High Docosahexaenoic Acid by &lt;i&gt;Schizochytrium&lt;/i&gt; sp. Using Low-cost Raw Materials from Food Industry

Song, Xiaojin; Zang, Xiaonan; Zhang, Xuecheng

doi:10.5650/jos.ess14164

Cited by 43 publications

(15 citation statements)

References 26 publications

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“…Algae also fix CO 2 into biomass during photosynthesis, thus addressing concerns about the generation of carbon emissions. Additionally, a wide range of by-products useful for biotechnological applications are produced in algae, notably replacing an increasing amount of the docosahexaenoic acid (C22:6 v3) market as the supply of fish oil dwindles (Morita et al, 2006;Song et al, 2015).…”

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confidence: 99%

Identification and Metabolite Profiling of Chemical Activators of Lipid Accumulation in Green Algae

Wase

Allen

et al. 2017

Plant Physiol.

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Microalgae are proposed as feedstock organisms useful for producing biofuels and coproducts. However, several limitations must be overcome before algae-based production is economically feasible. Among these is the ability to induce lipid accumulation and storage without affecting biomass yield. To overcome this barrier, a chemical genetics approach was employed in which 43,783 compounds were screened against Chlamydomonas reinhardtii, and 243 compounds were identified that increase triacylglyceride (TAG) accumulation without terminating growth. Identified compounds were classified by structural similarity, and 15 were selected for secondary analyses addressing impacts on growth fitness, photosynthetic pigments, and total cellular protein and starch concentrations. TAG accumulation was verified using gas chromatographymass spectrometry quantification of total fatty acids, and targeted TAG and galactolipid measurements were performed using liquid chromatography-multiple reaction monitoring/mass spectrometry. These results demonstrated that TAG accumulation does not necessarily proceed at the expense of galactolipid. Untargeted metabolite profiling provided important insights into pathway shifts due to five different compound treatments and verified the anabolic state of the cells with regard to the oxidative pentose phosphate pathway, Calvin cycle, tricarboxylic acid cycle, and amino acid biosynthetic pathways. Metabolite patterns were distinct from nitrogen starvation and other abiotic stresses commonly used to induce oil accumulation in algae. The efficacy of these compounds also was demonstrated in three other algal species. These lipid-inducing compounds offer a valuable set of tools for delving into the biochemical mechanisms of lipid accumulation in algae and a direct means to improve algal oil content independent of the severe growth limitations associated with nutrient deprivation.

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confidence: 99%

Identification and Metabolite Profiling of Chemical Activators of Lipid Accumulation in Green Algae

Wase

Allen

et al. 2017

Plant Physiol.

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“…[2] Theb iosynthetic genes for DHA, EPA, and ARA have been identified in marine bacteria: Moritella marina, Photobacterium profundum,a nd Aureispira marina,r espectively ( Figure 1B). [4] TheP UFAs ynthases identified in these organisms are composed of three or four subunits and possess similar domain structures including acyltransferase (AT), acyl carrier protein (ACP), malonyl CoA transacylase (MAT), b-ketoacyls ynthase (KS), ketoacyl reductase (KR), dehydratase (DH), and enoyl reductase (ER) domains in am anner similar to fatty-acid synthases (FASs;F igure 2). [4] TheP UFAs ynthases identified in these organisms are composed of three or four subunits and possess similar domain structures including acyltransferase (AT), acyl carrier protein (ACP), malonyl CoA transacylase (MAT), b-ketoacyls ynthase (KS), ketoacyl reductase (KR), dehydratase (DH), and enoyl reductase (ER) domains in am anner similar to fatty-acid synthases (FASs;F igure 2).…”

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confidence: 99%

Control Mechanism for Carbon‐Chain Length in Polyunsaturated Fatty‐Acid Synthases

et al. 2019

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“…The Algevir system was initially tested with the marine microalgae Schizochytrium sp., which is grown heterotrophycally and currently produced at an industrial scale; thus the adoption and scale-up of this technology will be greatly facilitated (Qu et al, 2013; Song et al, 2015). In conclusion, the Algevir system is a robust approach for producing recombinant proteins in microalgae that will open a new path in algal biotechnology.…”

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confidence: 99%

Algevir: An Expression System for Microalgae Based on Viral Vectors

et al. 2017

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The use of recombinant algae for the production of valuable compounds is opening promising biotechnological applications. However, the development of efficient expression approaches is still needed to expand the exploitation of microalgae in biotechnology. Herein, the concept of using viral expression vectors in microalgae was explored for the first time. An inducible geminiviral vector leading to Rep-mediated replication of the expression cassette allowed the production of antigenic proteins at high levels. This system, called Algevir, allows the production of complex viral proteins (GP1 from Zaire ebolavirus) and bacterial toxin subunits (B subunit of the heat-labile Escherichia coli enterotoxin), which retained their antigenic activity. The highest achieved yield was 1.25 mg/g fresh biomass (6 mg/L of culture), which was attained 3 days after transformation. The Algevir system allows for a fast and efficient production of recombinant proteins, overcoming the difficulties imposed by the low yields and unstable expression patterns frequently observed in stably transformed microalgae at the nuclear level; as well as the toxicity of some target proteins.

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Production of High Docosahexaenoic Acid by <i>Schizochytrium</i> sp. Using Low-cost Raw Materials from Food Industry

Cited by 43 publications

References 26 publications

Identification and Metabolite Profiling of Chemical Activators of Lipid Accumulation in Green Algae

Identification and Metabolite Profiling of Chemical Activators of Lipid Accumulation in Green Algae

Control Mechanism for Carbon‐Chain Length in Polyunsaturated Fatty‐Acid Synthases

Algevir: An Expression System for Microalgae Based on Viral Vectors

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