Production of Hemolysin and Bacteriolysin in a Synthetic Medium by Streptococcus faecalis var. zymogenes

Abstract

A synthetic medium has been developed in which Streptococcus faecalis var. zymogenes X-14 elaborates a lysin. The medium consists of 18 amino acids, lactose, six vitamins, adenine, guanine, uracil, and six salts. Glucose, and K,HPO, in excess of 0.5% (wt/vol), were inhibitory to lysin production; increasing concentrations of L-arginine-hydrochloride gave increasing yields of lytic activity. Brock et al. (4), in a survey of the bacteriocins of the enterococci, described a bacteriocin from ' Authorized for publication by The Experiment Station, Pennsylvania State University, as paper no. 4560.

Genetic loci of hemolysin production in Streptococcus faecalis subsp. zymogenes

“…After 4 h of incubation of the mixed culture of L2C3 Cmp" Eror, the number of Cmpr pr organisms had dropped from an initial concentration of 107 to JH2Eroe + L2 0 X-14-5 + L2 0 103 organisms/ml. We examined 19 of these Cmpr pr organisms and found that 1 was Hem+ and 18 were Hem-. All of the Hemorganisms were cross-streaked with strain A18 and also found to be lysin negative.…”