Production of Guanosine by Psicofuranine and Decoyinine Resistant Mutants of<i>Bacillus subtilis</i>

Matsui, Hiroshi; Sato, Katsuaki; Enei, Hitoshi; Hirose, Yoshio

doi:10.1080/00021369.1979.10863695

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…To test this, we repeated the long-term survival assays in DMEM but included decoyinine, an inhibitor of the GMP synthase GuaA. This inhibitor has been shown to artificially lower GTP levels and promote derepression of CodY in other organisms ( 43 to 45 ), which we hypothesize will negate the need for the (p)ppGpp 0 strain to mutate and form small colonies. When the wild-type JE2 was grown with decoyinine, no effect on survival was observed.…”

Section: Resultsmentioning

confidence: 99%

Stringent Response-Mediated Control of GTP Homeostasis Is Required for Long-Term Viability of Staphylococcus aureus

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Stringent Response-Mediated Control of GTP Homeostasis Is Required for Long-Term Viability of Staphylococcus aureus

et al. 2023

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“…Anti-neoplastic and antibiotic agent. Anti-bacterial activity [ 30 ] 16 Herbimycin Benzoquinone ansamycin antibiotic Potent herbicidal activity against most mono- and di-cotyledonous plants [ 31 ] 17 Leuseramycin Polyether antibiotic Anti-fungal activity [ 32 ] 18 Demalonylcopiamycin Macrolide antibiotic Anti-bacterial activity [ 33 ] 19 Pentalenolactone I Sesquiterpene Immunosuppressant [ 34 ] 20 Hygromycin Aminoglycoside Anti-bacterial, Immunosuppressant [ 35 ] 21 Hydantocidin Spironucleosides. Herbicidal activity [ 36 ] 22 Trialaphos Organophosphate’s pesticides Antibiotic [ 37 ] 23 Gopalamycin Polyether antibiotic Anti-fungal activity against Brown rust [ 38 ] 24 Validamycin Aminocyclitol antibiotics Antifungal and antibacterial against Brown rust activity [ 39 ] 25 …”

Section: Introductionmentioning

confidence: 99%

Molecular and therapeutic insights of rapamycin: a multi-faceted drug from Streptomyces hygroscopicus

Ganesh

Devi

2023

Mol Biol Rep

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The advancement in pharmaceutical research has led to the discovery and development of new combinatorial life-saving drugs. Rapamycin is a macrolide compound produced from Streptomyces hygroscopicus. Rapamycin and its derivatives are one of the promising sources of drug with broad spectrum applications in the medical field. In recent times, rapamycin has gained significant attention as of its activity against cytokine storm in COVID-19 patients. Rapamycin and its derivatives have more potency when compared to other prevailing drugs. Initially, it has been used exclusively as an anti-fungal drug. Currently rapamycin has been widely used as an immunosuppressant. Rapamycin is a multifaceted drug; it has anti-cancer, anti-viral and anti-aging potentials. Rapamycin has its specific action on mTOR signaling pathway. mTOR has been identified as a key regulator of different pathways. There will be an increased demand for rapamycin, because it has lesser adverse effects when compared to steroids. Currently researchers are focused on the production of effective rapamycin derivatives to combat the growing demand of this wonder drug. The main focus of the current review is to explore the origin, development, molecular mechanistic action, and the current therapeutic aspects of rapamycin. Also, this review article revealed the potential of rapamycin and the progress of rapamycin research. This helps in understanding the exact potency of the drug and could facilitate further studies that could fill in the existing knowledge gaps. The study also gathers significant data pertaining to the gene clusters and biosynthetic pathways involved in the synthesis and production of this multi-faceted drug. In addition, an insight into the mechanism of action of the drug and important derivatives of rapamycin has been expounded. The fillings of the current review, aids in understanding the underlying molecular mechanism, strain improvement, optimization and production of rapamycin derivatives. Graphical abstract

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“…However, MSG is extremely important for the umami taste 1 in foods, worldwide, and the amount of production is about 100 times greater than 5 0 -nucleotides. Because purine nucleosides, such as guanosine 2 and inosine, 3 can be efficiently produced by fermentation, the progress of large-scale 5 0 -nucleotide production mainly depends on improving the nucleoside-phosphorylation process.…”

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Structural-Based Engineering for Transferases to Improve the Industrial Production of 5′-Nucleotides

Suzuki¹,

Ishikawa²,

Mihara³

et al. 2007

Bulletin of the Chemical Society of Japan

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The existence of a flexible region for substrate recognition by an enzyme was first discovered through a structure comparison study among ribonuclease T1-related molecules, including intact molecules, intact molecules complexed with 2 0 -guanylic acid (GMP), and carboxymethylated molecules complexed with 2 0 -GMP. Next, it was demonstrated that transglutaminase activity could be enhanced by identifying the flexible residues that affect the active site using nuclear magnetic resonance. Finally, such an approach was used to increase the production of 5 0 -inosinic acid (5 0 -IMP) and 5 0 -GMP. The enzyme is a nonspecific acid phosphatase with pyrophosphate-nucleoside phosphotransferase activity that is C-5 0 position-selective. The crystal structure of G74D/I153T with a reduced K M is virtually identical to that of the wild type, and neither of the side chains that were introduced Asp74 and Thr153, directly interact with the nucleoside, although both residues are situated near a potential nucleoside binding site. In addition, the two regions around residues 70 and 140 have the most flexible conformational rearrangement upon phosphate analogue binding. On the basis of three-dimensional (3D) structural information, some practical, high-performance enzymes for 5 0 -nucleotides production were discovered, and new varieties of microorganisms were developed.

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Production of Guanosine by Psicofuranine and Decoyinine Resistant Mutants ofBacillus subtilis

Cited by 3 publications

References 5 publications

Stringent Response-Mediated Control of GTP Homeostasis Is Required for Long-Term Viability of Staphylococcus aureus

Stringent Response-Mediated Control of GTP Homeostasis Is Required for Long-Term Viability of Staphylococcus aureus

Molecular and therapeutic insights of rapamycin: a multi-faceted drug from Streptomyces hygroscopicus

Structural-Based Engineering for Transferases to Improve the Industrial Production of 5′-Nucleotides

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