Production of glutamic acid by Corynebacterium glutamicum using dates syrup as carbon source

Ahmed, Shahzad; M, Y; Khan, M. R.; J, A; Abulnaja,; K, A; Malki, None Al; A.A., Loginova

doi:10.5897/ajmr12.2223

Cited by 9 publications

(9 citation statements)

References 9 publications

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“…Penicillin was used to permeabilize C . glutamicum during glutamic acid fermentation [ 31 ] and toluene was an efficient permeabilizer to G . suboxydans for the production of l -sorbose [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

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D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii

et al. 2016

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A d-allulose 3-epimerase from Flavonifractor plautii was cloned and expressed in Escherichia coli and Corynebacterium glutamicum. The maximum activity of the enzyme purified from recombinant E. coli cells was observed at pH 7.0, 65°C, and 1 mM Co2+ with a half-life of 40 min at 65°C, Km of 162 mM, and kcat of 25280 1/s. For increased d-allulose production, recombinant C. glutamicum cells were permeabilized via combined treatments with 20 mg/L penicillin and 10% (v/v) toluene. Under optimized conditions, 10 g/L permeabilized cells produced 235 g/L d-allulose from 750 g/L d-fructose after 40 min, with a conversion rate of 31% (w/w) and volumetric productivity of 353 g/L/h, which were 1.4- and 2.1-fold higher than those obtained for nonpermeabilized cells, respectively.

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Section: Resultsmentioning

confidence: 99%

“…Although penicillin has been used in glutamic acid fermentation to permeabilize C . glutamicum [ 30 , 31 ], cell permeabilization with antibiotics has not yet been applied to whole-cell bioprocesses.…”

Section: Introductionmentioning

confidence: 99%

D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii

et al. 2016

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“…Wild type glutamic acid-producing bacteria used in this study produced a low yield of the amino acid when compared with the L-glutamic yield by Corynebacterium glutamicum ATCC 13032 (Lawal et al, 2011) and Corynebacterium glutamicum AJ1510 (Ahmed et al, 2013). This could be due to the fact that the natural overproduction of amino acids is rare because the metabolic pathway for the production is tightly controlled and operates economically.…”

Section: Discussionmentioning

confidence: 89%

A Study of Naturally-occurring L-glutamic Acid Producing Bacteria From Tropical Soil and Aquatic Environments.

Adeogun

Omotayo

Oderinde

et al. 2017

Egyptian Academic Journal of Biological Sciences, G. Microbiolo

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Amino acids have been commercially harnessed as feed supplement, infusion compounds, therapeutic agents and precursors for the synthesis of peptides or agrochemicals (Shyamkumar et al., 2014). Interest in L-glutamic acid as the first amino acid to be produced by fermentation on a large scale was stimulated by the increasing demand for monosodium glutamate as a flavour enhancing agent. It is presently consumed worldwide in the form of monosodium salt as a flavour enhancer in foods (Ikeda, 2003). Microbial means of Lglutamic acid production have the advantage of yielding exclusively optically active and biologically required L-form of glutamic acid directly (Amin and Al-Talhi, 2007). Corynebacterium sp., Brevibacterium sp., Microbacterium sp. are among the patent glutamic acid-producing strains by direct fermentation and are collectively referred to as glutamic acid bacteria (Okamoto and Ikeda, 2000).

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“…Glucose concentration: Many published articles have suggested the use of different sources of glucose can stimulate C. glutamicum growth and induce metabolic processes [37,38,39,40] and Naiyf S. Alharbi rt al. (2020) Showed that pure glucose has the highest efficiency of glutamic acid production by C. glutamicum13032 [34].…”

Section: Discussionmentioning

confidence: 99%

Application of RSM for optimization of glutamic acid production by Corynebacterium glutamicum in bath culture

Fahimitabar

Razavian

Rezaei

2021

Heliyon

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Glutamate plays an important role in different cellular processes. Its new applications in various industries have led to an increase in the production of it while fermentation is a very important economically method. In this study, the production of glutamate by the wild type of Corynebacterium glutamicum PTCC(Persian Type Culture Collection) 1532 was optimized using RSM.Central Composite Design (CCD) was developed by Design-Expert software version 12.0.3.0 (dx-12, State-Ease Inc.) to evaluate the effect of four important variables in five levels on glutamate production. TLC was employed to evaluate glutamate in medium qualitatively and then quantitative estimation was done by HPLC.Normal probability analysis demonstrated that data has a normal distribution. The results of ANOVA analysis showed that the urea concentration both alone and with temperature is the most effective variable in the fermentation process. Based on the quadratic model obtained in CCD, temperature 30 C; glucose 9 g.dL À1 ; biotin 9 μg.L À1 and urea concentration of 0.3 g.dL À1 were found optimum conditions with a predicted glutamate production of 19.84 mg.mL À1 with desirable level 1.Therefore RSM can be an effective method to optimize glutamate production and the findings of this study are a guideline for the other amino acids fermentation by C. glutamicum.

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Production of glutamic acid by Corynebacterium glutamicum using dates syrup as carbon source

Cited by 9 publications

References 9 publications

D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii

D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii

A Study of Naturally-occurring L-glutamic Acid Producing Bacteria From Tropical Soil and Aquatic Environments.

Application of RSM for optimization of glutamic acid production by Corynebacterium glutamicum in bath culture

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