Production of glucose-rich enzymatic hydrolysates from cellulosic pulps

Buzała, Kamila Przybysz; Przybysz, Piotr; Rosicka‐Kaczmarek, Justyna; Kalinowska, Halina

doi:10.1007/s10570-014-0522-x

Cited by 31 publications

(27 citation statements)

References 18 publications

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“…The latter preparation was selected in a former study (Buzała et al 2015a) as an efficient biocatalyst, enabling the nearly complete hydrolysis of various cellulosic pulps and fines from papermaking as well as partial hydrolysis of diverse woodchips and chopped wheat straw. This preparation displays the activities of cellulases (80.6 U/mL for CMC and 112.12 FPU/mL at 50°C and pH 5.0) and xylanases (192.5 U/mL at 50°C and pH 5.0) (Buzała et al 2015b).…”

Section: Enzymatic Hydrolysis Of Cellulosic Pulpsmentioning

confidence: 99%

Conversion of various types of lignocellulosic biomass to fermentable sugars using kraft pulping and enzymatic hydrolysis

et al. 2017

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The aim of this work was to assess the utility of seven different kraft pulps produced from softwood (pine), hardwood (poplar, birch and beech), wheat straw and hemp (bast and harl) as potential sources of sugar feedstocks for fermentation processes. The pulps contained low amounts of hemicelluloses (1.9-8.2% d.w.) and lignin (1.7-15% d.w.). The crystallinity index values ranged from 55% (wheat straw pulp) to 79% (hemp bast pulp), while the average DP varied from around 230 (hemp bast pulp) to 1482 (poplar and birch pulps). The results of enzymatic hydrolysis showed that not only the residual lignin content but also the cellulose crystallinity index decided on the sugar yields while the average polymerization degree had a weak impact. More reducing sugars were obtained from the hardwood pulps and wheat straw pulp (100% d.w.) than from the pine pulp (around 89% d.w.) and two hemp pulps (40.5% d.w. and 44.7% d.w. from the bast and harl pulps, respectively). Glucose was the dominating (69-79% w/w) soluble sugar in enzymatic hydrolysates of the pulps. The sugar profiles of these hydrolysates make them suitable sugar feedstocks for fermentation processes.

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Section: Enzymatic Hydrolysis Of Cellulosic Pulpsmentioning

confidence: 99%

Conversion of various types of lignocellulosic biomass to fermentable sugars using kraft pulping and enzymatic hydrolysis

et al. 2017

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“…The preparation NS-22086, which was used in this study for hydrolysis of pine cellulosic pulps with Kappa numbers ranging from 17.2 to 86.2, pine woodchips (0.43-0.8 mm) and fines from paper mill, was selected as described by Buzała et al (2015). It displayed the activities of cellulases and xylanases (Table 2).…”

Section: Enzyme Preparationmentioning

confidence: 99%

Evaluation of pine kraft cellulosic pulps and fines from papermaking as potential feedstocks for biofuel production

et al. 2015

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Results of enzymatic hydrolysis of pine kraft cellulosic pulps (Kappa numbers ranging from 17.2 to 86.2) and waste fines from paper production line suggest that they are potential feedstocks for biofuel production. Glucose (61-68 % d.w.) and reducing sugars (around 90 % d.w.) yields from the pine cellulosic pulps with Kappa numbers ranging between 17.2 and 47.7 were higher than from the two pulps with Kappa numbers of 60.9 and 86.2. Glucose and reducing sugars yields from the latter pulps were around 54 and 45 % d.w. and 78 and 67 % d.w., respectively. The highest yields of glucose and total reducing sugars on a pine wood dry weight basis (29.3 and 43.1 %, respectively) were derived from the pulp with the Kappa number of 47.7. Thus the complete lignin removal from pine wood was not necessary to obtain maximum yields of glucose and other reducing sugars. Enzymatic hydrolysis of the fines from pine wood processing in a paper mill yielded around 75 % glucose and 78 % total reducing sugars on a dry weight basis that corresponded to around 34 and 36 % on a pine wood dry weight basis.

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“…Poplar and pine cellulosic pulps were prepared by the sulfate method as described elsewhere (Buzała et al 2015) from woodchips (25 mm 9 15 mm 9 8 mm), containing 7-8 % humidity. The chips were obtained from wood after the inner and outer barks and knots were mechanically removed.…”

Section: Kraft Wood Pulpsmentioning

confidence: 99%

“…Their content in enzymatic hydrolysates depends on conditions of biomass pretreatment. For instance, enzymatic hydrolysates of hardwood and softwood cellulosic pulps derived by kraft pulping are rich in glucose, constituting above 73 % soluble sugars (Buzała et al 2015).…”

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“…In this study, the hydrolysis of wood pulps and chips was mediated by a multienzyme preparation NS-22086, which contains enzymes enabling conversion of cellulose and hemicelluloses to simple sugars (Buzała et al 2015). Our previous study showed that this preparation efficiently degraded kraft cellulosic pulps from wood (poplar, pine, beech and birch) and wheat straw as well as waste fines from paper mill.…”

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Comparison of digestibility of wood pulps produced by the sulfate and TMP methods and woodchips of various botanical origins and sizes

et al. 2015

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Poplar and pine cellulosic pulps derived by the sulfate process (Kappa numbers of 15.4 and 31.4, respectively) and a poplar thermomechanical pulp (TMP, Kappa number of 124.7) as well as 0.43-0.8 mm woodchips of various botanical origins (poplar, birch, lime, oak, beech, pine, and spruce) and poplar chips of five different average particle sizes, ranging from 1.6-2.0 to\0.43 mm, were digested by a commercial preparation of cellulases and xylanases (NS-22086 from Novozymes). Yields of reducing sugars derived by enzymatic hydrolysis of the poplar and pine pulps that were obtained by the sulfate method achieved 100 and 89 % on a dry weight basis (52.3 and 35.9 % d.w. wood, respectively) while glucose yields reached 79 and 70.7 % d.w. pulp (41.3 and 28.6 % d.w. wood), respectively. Enzymatic hydrolysis of the pine TMP resulted in glucose and reducing sugars yields of only 14.0 and 36.7 % d.w. pulp (around 13 and 34 % d.w. wood, respectively). Yields of glucose and soluble reducing sugars released from the poplar chips were inversely proportional to their size and varied between around 5.3-7.8, and 15.7-22.4 % d.w., respectively. Reducing sugars yields from beech, birch and lime chips were slightly higher than from the poplar chips of the same size while pine, spruce and oak chips were less susceptible to enzymatic digestion. The dominating sugars in the hydrolysates of woodchips were glucose and cellobiose (70-90 % w/w of the sum of 5 detected sugars) while mannose, xylose and arabinose concentrations were relatively low.

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Production of glucose-rich enzymatic hydrolysates from cellulosic pulps

Cited by 31 publications

References 18 publications

Conversion of various types of lignocellulosic biomass to fermentable sugars using kraft pulping and enzymatic hydrolysis

Conversion of various types of lignocellulosic biomass to fermentable sugars using kraft pulping and enzymatic hydrolysis

Evaluation of pine kraft cellulosic pulps and fines from papermaking as potential feedstocks for biofuel production

Comparison of digestibility of wood pulps produced by the sulfate and TMP methods and woodchips of various botanical origins and sizes

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