Production of gellan gum, an exopolysaccharide, from biodiesel-derived waste glycerol by Sphingomonas spp.

Abstract

In the present study, biodiesel-derived waste glycerol (WG) was used for the isolation and production of gellan, an exopolysaccharide, on media containing WG as the main carbon source. Two bacterial isolates showed gellan producing potential which were identified as (Accession No. GI:724472387) and (GI:724472388) by 16S rRNA gene sequencing. To maximize gellan production by and, media optimization was performed at different pHs and glycerol concentrations. Morphological observations through microscopic images showed the production of gellan from these isolates. Simple linear regression showed better utilization of WG by than at pH 6 and pH 7. Though, both the strains showed reverse trend at pH 8. Both the strains were able to produce high amounts of gellan gum (51.6 and 52.6 g/l, respectively) using WG (80 g/l) as the sole carbon source, in a minimal medium. This is the first report on the efficient degradation of WG and low-cost production of gellan. Owing to these characteristics, and demonstrate great potential for use in the commercial production of gellan and in the bioremediation of WG.

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“…The quality of life on Earth is inevitably related to the overall quality of the environment. As human activity has increased around the globe, the Earth has been contaminated with a large number of toxic pollutants from multiple sources (Raghunandan et al, 2014 , 2018 ). The shrinking of natural resources, an increase in pollution and carbon emissions and other problems related to human health are the consequence of industrialization and have proven disastrous for every global region (Ahuti, 2015 ).…”

“…To safeguard both humans and environment from the adverse consequences of environmental pollution novel approaches must be designed, and bioremediation is one such approach. Interest in the microbial-based bioremediation of contaminants has increased in recent years, as people endeavor to find sustainable ways of remediating polluted environments (Raghunandan et al, 2014 , 2018 ; Kumar et al, 2016 ). The biotransformation and bioremediation-based methods strive to harness the naturally occurring microbial catabolic diversity to degrade, transform or accumulate vast amounts of problematic compounds, including radionuclides, metals, pharmaceutical substances, polyaromatic hydrocarbons (PAHs), and polychlorinated biphenyls (PCBs).…”

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“…Further, their associated negative impacts of fossil fuels on global climate are also becoming environmental concerns in many countries. Biofuels in lieu of fossil fuels has drawn a tremendous attention as a source of renewable, and clean energy (Kumar et al, 2010 ; Kumar and Sharma, 2011 ; Singh et al, 2014a ; Raghunandan et al, 2018 ). Therefore, the search of alternative renewable energy source for biofuel production has expanded (Rezania et al, 2015 ).…”

Fungal Population Dynamics Associated with Active-phase of Hydrocarbon Degradation in Oil-polluted Soil

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